Cell-permeable (icp)-socs3 recombinant protein and uses thereof

ABSTRACT

Provided are an improved cell-permeable (iCP)-SOCS3 recombinant protein and uses thereof. Suppressor of cytokine signaling-3 (SOCS3) is an endogenous protein inhibitor of JAK/STAT pathway, and an aberrant expression of SOCS3 protein was observed in human solid tumors including gastric, colorectal and breast cancer, and glioblastoma. Thus, the iCP-SOCS3 recombinant protein may be used as protein-based anti-solid tumor agent by utilizing the platform technology for macromolecule intracellular transduction.

CROSS REFERENCE TO RELATED APPLICATIONS

This is Divisional Application of U.S. application Ser. No. 15/361,701filed Nov. 28, 2016, which is a Continuation-in-Part of application Ser.No. 14/838,304 filed Aug. 27, 2015, which claims the benefit of thefiling date of U.S. Provisional Application No. 62/042,493, filed onAug. 27, 2014, in the United States Patent and Trademark Office, thedisclosure of which is incorporated herein in its entirety by reference.

TECHNICAL FIELD

The present invention relates to providing improved cell-permeable(iCP)-SOCS3 recombinant protein and uses thereof. Preferably, theiCP-SOCS3 recombinant protein may be used as protein-based anti-solidtumor agent by utilizing the platform technology for macromoleculeintracellular transduction.

BACKGROUND ART

The Janus kinase signal transducers and activators of transcriptionsignaling (JAK/STAT) plays important roles in immune responses,including oncogenesis. So many investigations demonstrated that STAT-3,an important member of STAT proteins, was considered as a proto-oncogenein various types of disorder. STAT-3 is phosphorylated and dimerizes bythe Janus kinase (JAK), and its overexpression and constitutiveactivation can significantly induce cell proliferation, tumorangiogenesis, invasion. Meanwhile, inhibition of JAK-STAT signaling ledto suppress the cancer cell growth and induce apoptosis. Suppressor ofcytokine signaling-3 (SOCS3), a kind of endogenous protein inhibitor ofJAK/STAT pathway, was identified to be inversely associated with theSTAT3 expression and phosphorylation in vivo and in vitro and aberrantexpression of SOCS3 protein was observed in human solid tumors includinggastric, colorectal and breast cancer, and glioblastoma.

Gastric cancer remains the second leading cause of cancer-related deathin the world. Advances in early detection and decreased chronicHelicobacter pylori infection rates have led to a substantial reductionin gastric cancer rates worldwide. However, effective treatment regimensfor gastric cancers, especially advanced gastric cancer, are stilllacking; therefore, the prognosis of patients with this disease remainspoor. SOCS3 mRNA levels are higher in adjacent normal mucosal tissues,however, gastric cancer patients with high simultaneous expression ofSOCS3 have a better overall survival than those with low simultaneousexpression. Based on this, SOCS3 may represent new therapeutic target totreat gastric cancer.

Colorectal cancer is one of the most fatal neoplastic diseases worldwideand a serious global health problem, with over one million new cases andhalf million mortalities worldwide each year. It has been reported asbeing relevant to some inflammatory bowel diseases, such as Crohn'sdisease and ulcerative colitis. The pathogenesis of colorectal carcinomais complex, with the involvement of multiple cellular transductionpathways including IL-6/STAT3 signaling. Reduced or silenced SOCS3 hasbeen found in many human types of cancer including colorectal cancer,and restoring SOCS3 expression in the cancer cells inhibitsIL-6-mediated STAT3 activation, induces tumor cell apoptosis anddecreases cell proliferation. Therefore, suppression of the IL-6/STAT3pathway via modulation of SOCS3 has been a promising strategy foranti-colon/colorectal cancer therapy.

Glioblastoma, the most common neoplasm among diffuse infiltratingastrocytomas, is notorious for its ability to evade immune-surveillanceas well as for its invasive and angiogenic properties. Gliomas are themost common type of primary brain tumors are highly malignant and areassociated with a very poor prognosis. Glioblastoma is a very aggressivesubtype of glioma with very short life expectancy and limited treatmentoptions. A hallmark of this lethal disorder is the presence of activatedSTAT3. Because SOCS3 is a negative regulator of STAT-3 activation, ithypothesized that SOCS3 may function as a tumor suppressor inglioblastoma tissues.

Breast cancer is a disease that arises from the accumulation ofalterations in the genome of cells that make up the mammary gland.Breast cancer is the most common type of cancer among women, with anestimated 1.38 million new cases of cancer diagnosed in 2008 (23% of allcancers), and the second most common type of cancer overall (10.9% ofall cancers). Expression of SOCS3 protein is significantlydown-regulated in breast cancer specimens and replacing of SOCS3 proteinmay directly influence the treatment of breast cancer.

Cytokine signaling is strictly regulated by the SOCS family proteinsinduced by different classes of agonists, including cytokines, hormonesand infectious agents. Among them, SOCS1 and SOCS3 are relativelyspecific to STAT1 and STAT3, respectively. SOCS1 inhibits JAK activationthrough its N-terminal kinase inhibitory region (KIR) by the directbinding to the activation loop of JAKs, while SOCS3 binds to januskinases (JAKs)-proximal sites on the receptor through its SH2 domain andinhibits JAK activity that blocks recruitment of STAT3. Both promoteanti-inflammatory effects due to the suppression ofinflammation-inducing cytokine signaling. Furthermore, the SOCS box,another domain in SOCS proteins, interacts with E3 ubiquitin ligasesand/or couples the SH2 domain-binding proteins to theubiquitin-proteasome pathway. Therefore, SOCSs inhibit cytokinesignaling by suppressing JAK kinase activity and degrading the activatedcytokine receptor complex.

In connection with SOCSs and various solid tumors including gastric,colorectal and breast cancer, and glioblastoma, the SOCS1 gene has beenimplicated as an anti-oncogene in the tumor development. Previousstudies have reported that aberrant methylation in the CpG island ofSOCS1 induces its transcriptional silencing in cancer cell lines, andSOCS1 heterozygous mice are hypersensitive to various cancers. Inaddition, abnormalities of SOCS3 are also associated with the solidtumors. Hypermethylation of CpG islands in the SOCS3 promoter iscorrelated with its transcriptional silencing in tumors cell lines.SOCS3 overexpression down-regulates active STAT3, induces apoptosis, andsuppresses growth in cancer cells. The importance of STAT3 toinflammation-associated carcinogenesis is underlined by the previousstudy that cancer-specific deletion of SOCS3 in a mouse carcinoma modelresults in larger and more numerous tumors. This means that SOCS3 playsa major role in the negative regulation of the JAK/STAT pathway incarcinogenesis and contributes to the suppression of tumor developmentby protecting the tissue cells.

REFERENCES

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DISCLOSURE Technical Problem

To negatively control JAK/STAT signaling, recombinant SOCS3 proteinsthat contain a cell-penetrating peptide (CPP)-membrane-translocatingmotif (MTM) from fibroblast growth factor (FGF)-4 has been reported.These recombinant SOCS3 proteins inhibited STAT phosphorylation,inflammatory cytokines production and MHC-II expression in cultured andprimary macrophages. In addition, SOCS3 fused to MTM protected micechallenged with a lethal dose of the SEB super-antigen, by suppressingapoptosis and hemorrhagic necrosis in multiple organs. However, theSOCS3 proteins fused to FGF4-derived MTM displayed extremely lowsolubility, poor yields and relatively low cell- andtissue-permeability. Therefore, the MTM-fused SOCS3 proteins were notsuitable for further clinical development as therapeutic agents.

Technical Solution

For MITT, six critical factors (length, bending potential, instabilityindex, aliphatic index, GRAVY, amino acid composition) have beendetermined through analysis of baseline hydrophobic CPPs. Advancedmacromolecule transduction domain (aMTD), newly designed based on thesesix critical factors, could optimize cell-/tissue-permeability of SOCS3proteins that have a therapeutic effects and develop them asprotein-based drugs. Further, in order to increase solubility and yieldof recombinant protein, solubilization domains (SDs) additionally fusedto the aMTD-SOCS3 recombinant protein, thereby notably increased thesolubility and manufacturing yield of the recombinant protein.

In this application, aMTD/SD-fused iCP-SOCS3 recombinant proteins(iCP-SOCS3), much improved physicochemical characteristics (solubilityand yield) and functional activity (cell-/tissue-permeability) comparedwith the protein fused only to FGF-4-derived MTM. In addition, the newlydeveloped iCP-SOCS3 proteins have now been demonstrated to havetherapeutic application in treating solid tumor, exploiting the abilityof SOCS3 to suppress JAK/STAT signaling. The present applicationrepresents that macromolecule intracellular transduction technology(MITT) enabled by the new hydrophobic CPPs that are aMTD may providenovel protein therapy through SOCS3-intracellular protein replacementagainst the solid tumor. These findings suggest that restoration ofSOCS3 by replenishing the intracellular SOCS3 with iCP-SOCS3 proteincreates a new paradigm for anti-cancer therapy, and the intracellularprotein replacement therapy with the SOCS3 recombinant protein fused tothe combination of aMTD and SD pair may be useful to treat the solidtumor.

One aspect disclosed in the present application provides an improvedCell-Permeable (iCP)-SOCS3 recombinant protein, which comprises a SOCS3protein; and an advanced macromolecule transduction domain (aMTD) beingcomposed of 9-13 amino acid sequences and having improved cell and/ortissue permeability, wherein the aMTD is fused to one end or both endsof the SOCS3 protein and has the following features of:

(a) being composed of 3 or more amino acid sequences selected from thegroup consisting of Ala, Val, Ile, Leu, and Pro;

(b) having proline as amino acid sequences corresponding to any one ormore of positions 5 to 8, and 12 of its amino acid sequence; and

(c) having an instability index of 40-60; an aliphatic index of 180-220;and a grand average of hydropathy (GRAVY) of 2.1-2.6, as measured byProtparam.

According to one embodiment, one or more solubilization domain (SD)(s)are further fused to the end(s) of one or more of the SOCS3 protein andthe aMTD.

According to another embodiment, the aMTD may form α-Helix structure.According to still another embodiment, the aMTD may be composed of 12amino acid sequences and represented by the following general formula:

wherein X(s) independently refer to Alanine (A), Valine (V), Leucine (L)or Isoleucine (I); and Proline (P) can be positioned in one of U(s)(either 5′, 6′, 7′ or 8′). The remaining U(s) are independently composedof A, V, L or I, P at the 12′ is Proline.

Another aspect disclosed in the present application provides aniCP-SOCS3 recombinant protein which is represented by any one of thefollowing structural formulae:

A-B-C,A-C-B,B-A-C,B-C-A,C-A-B,C-B-A and A-C-B-C

wherein A is an advanced macromolecule transduction domain (aMTD) havingimproved cell and/or tissue permeability, B is a SOCS3 protein, and C isa solubilization domain (SD); and

the aMTD is composed of 9-13 amino acid sequences and has the followingfeatures of:

(a) being composed of 3 or more amino acids selected from the groupconsisting of Ala, Val, Ile, Leu, and Pro;

(b) having proline as amino acid sequences corresponding to any one ormore of positions 5 to 8, and 12 of its amino acid sequence;

(c) having an instability index of 40-60; an aliphatic index of 180-220;and a grand average of hydropathy (GRAVY) of 2.1-2.6, as measured byProtparam; and

(d) forming α-Helix structure.

According to one embodiment disclosed in the present application, theSOCS3 protein may have an amino acid sequence of SEQ ID NO: 814.

According to another embodiment disclosed in the present application,the SOCS3 protein may be encoded by a polynucleotide sequence of SEQ IDNO: 815.

According to still another embodiment disclosed in the presentapplication, the SOCS3 protein may further include a ligand selectivelybinding to a receptor of a cell, a tissue, or an organ.

According to still another embodiment disclosed in the presentapplication, the aMTD may have an amino acid sequence selected from thegroup consisting of SEQ ID NOs: 1-240 and 822.

According to still another embodiment disclosed in the presentapplication, the aMTD may be encoded by a polynucleotide sequenceselected from the group consisting of SEQ ID NOs: 241-480 and 823.

According to still another embodiment disclosed in the presentapplication, the SD(s) may have an amino acid sequence independentlyselected from the group consisting of SEQ ID NOs: 798, 799, 800, 801,802, 803, and 804.

According to still another embodiment disclosed in the presentapplication, the SD(s) may be encoded by a polynucleotide sequenceindependently selected from the group consisting of SEQ ID NOs: 805,806, 807, 808, 809, 810, and 811.

According to still another embodiment disclosed in the presentapplication, the iCP-SOCS3 recombinant protein may have a histidine-tagaffinity domain additionally fused to one end thereof.

According to still another embodiment disclosed in the presentapplication, the histidine-tag affinity domain may have an amino acidsequence of SEQ ID NO: 812.

According to still another embodiment disclosed in the presentapplication, the histidine-tag affinity domain may be encoded by apolynucleotide sequence of SEQ ID NO: 813.

According to still another embodiment disclosed in the presentapplication, the fusion may be formed via a peptide bond or a chemicalbond.

According to still another embodiment disclosed in the presentapplication, the iCP-SOCS3 recombinant protein may be used for thetreating, preventing, or delaying the onset of, solid tumor.

Still another aspect disclosed in the present application provides apolynucleotide sequence encoding the iCP-SOCS3 recombinant protein.

According to one embodiment disclosed in the present application, thepolynucleotide sequence may be a polynucleotide sequence represented bySEQ ID NO: 825.

Still another aspect disclosed in the present application provides arecombinant expression vector including the polynucleotide sequence.

Still another aspect disclosed in the present application provides atransformant transformed with the recombinant expression vector.

Still another aspect disclosed in the present application provides apreparing method of the iCP-SOCS3 recombinant protein includingpreparing the recombinant expression vector; preparing the transformantusing the recombinant expression vector; culturing the transformant; andrecovering the recombinant protein expressed by the culturing.

Still another aspect disclosed in the present application provides acomposition including the iCP-SOCS3 recombinant protein as an activeingredient.

Still another aspect disclosed in the present application provides apharmaceutical composition for the treating, preventing, or delaying theonset of, solid tumor including the iCP-SOCS3 recombinant protein as anactive ingredient; and a pharmaceutically acceptable carrier.

Still another aspect disclosed in the present application provides useof the iCP-SOCS3 recombinant protein as a medicament for the treating,preventing, or delaying the onset of, solid tumor.

Still another aspect disclosed in the present application provides amedicament including the iCP-SOCS3 recombinant protein.

Still another aspect disclosed in the present application provides useof the iCP-SOCS3 recombinant protein in the preparation of a medicamentfor the treating, preventing, or delaying the onset of, solid tumor.

Still another aspect disclosed in the present application provides amethod of treating, preventing, or delaying the onset of, solid tumor ina subject, the method including identifying a subject in need of thetreating, preventing, or delaying the onset of, solid tumor; andadministering to the subject a therapeutically effective amount of theiCP-SOCS3 recombinant protein.

According to one embodiment disclosed in the present application, thesubject may be a mammal.

Unless defined otherwise, all terms used herein have the same meaning ascommonly understood by one of ordinary skill in the art to which thepresent invention belongs. Although a certain method and a material isdescribed herein, it should not be construed as being limited thereto,any similar or equivalent method and material to those may also be usedin the practice or testing of the present invention. All publicationsmentioned herein are incorporated herein by reference in their entiretyto disclose and describe the methods and/or materials in connection withwhich the publications are cited. It must be noted that as used hereinand in the appended claims, the singular forms “a,” “an,” and “the”include plural referents unless the context clearly dictates otherwise.

A “peptide,” as used herein, refers to a chain-type polymer formed byamino acid residues which are linked to each other via peptide bonds,and used interchangeably with “polypeptide.” Further, a “polypeptide”includes a peptide and a protein.

Further, the term “peptide” includes amino acid sequences that areconservative variations of those peptides specifically exemplifiedherein. The term “conservative variation,” as used herein, denotes thereplacement of an amino acid residue by another, biologically similarresidue. Examples of conservative variations include substitution of onehydrophobic residue, such as isoleucine, valine, leucine, alanine,cysteine, glycine, phenylalanine, proline, tryptophan, tyrosine,norleucine, or methionine for another, or substitution of one polarresidue for another, for example, substitution of arginine for lysine,glutamic acid for aspartic acid, or glutamine for asparagine, and thelike. Neutral hydrophilic amino acids which may be substituted for oneanother include asparagine, glutamine, serine, and threonine.

The term “conservative variation” also includes use of a substitutedamino acid in place of an unsubstituted parent amino acid, provided thatantibodies raised to the substituted polypeptide also immunoreact withthe unsubstituted polypeptide. Such conservative substitutions arewithin the definition of the classes of the peptides disclosed in thepresent application.

A person having ordinary skill in the art may make similar substitutionsto obtain peptides having higher cell permeability and a broader hostrange. For example, one aspect disclosed in the present applicationprovides peptides corresponding to amino acid sequences (e.g. SEQ IDNOs: 1 to 240 and 822) provided herein, as well as analogues, homologs,isomers, derivatives, amidated variations, and conservative variationsthereof, as long as the cell permeability of the peptide remains.

Minor modifications to primary amino acid sequence disclosed in thepresent application may result in peptides which have substantiallyequivalent or enhanced cell permeability, as compared to the specificpeptides described herein. Such modifications may be deliberate, as bysite-directed mutagenesis, or may be spontaneous.

All peptides may be synthesized using L-amino acids, but D forms of allof the peptides may be synthetically produced. In addition, C-terminalderivatives, such as C-terminal methyl esters and C-terminal amidates,may be produced in order to increase the cell permeability of thepeptide according to one embodiment disclosed in the presentapplication.

All of the peptides produced by these modifications are included herein,as long as in the case of amidated versions of the peptide, the cellpermeability of the original peptide is altered or enhanced such thatthe amidated peptide is therapeutically useful. It is envisioned thatsuch modifications are useful for altering or enhancing cellpermeability of a particular peptide.

Furthermore, deletion of one or more amino acids may also result in amodification to the structure of the resultant molecule without anysignificant change in its cell permeability. This may lead to thedevelopment of a smaller active molecule which may also have utility.For example, amino- or carboxyl-terminal amino acids which may not berequired for the cell permeability of a particular peptide may beremoved.

The term “gene” refers to an arbitrary nucleic acid sequence or a partthereof having a functional role in protein coding or transcription, orregulation of other gene expression. The gene may be composed of allnucleic acids encoding a functional protein or a part of the nucleicacid encoding or expressing the protein. The nucleic acid sequence mayinclude a gene mutation in exon, intron, initiation or terminationregion, promoter sequence, other regulatory sequence, or a uniquesequence adjacent to the gene.

The term “primer” refers to an oligonucleotide sequence that hybridizesto a complementary RNA or DNA target polynucleotide and serves as thestarting points for the stepwise synthesis of a polynucleotide frommononucleotides by the action of a nucleotidyltransferase as occurs, forexample, in a polymerase chain reaction.

The term “coding region” or “coding sequence” refers to a nucleic acidsequence, a complement thereof, or a part thereof which encodes aparticular gene product or a fragment thereof for which expression isdesired, according to the normal base pairing and codon usagerelationships. Coding sequences include exons in genomic DNA or immatureprimary RNA transcripts, which are joined together by the cellularbiochemical machinery to provide a mature mRNA. The anti-sense strand isthe complement of the nucleic acid, and the coding sequence may bededuced therefrom.

One aspect disclosed in the present application provides an iCP-SOCS3recombinant protein, which comprises a SOCS3 protein and an advancedmacromolecule transduction domain (aMTD) being composed of 9-13 aminoacid sequences, preferably 10-12 amino acid sequences, and havingimproved cell and/or tissue permeability, wherein the aMTD is fused toone end or both ends of the SOCS3 protein and has the following featuresof:

(a) being preferably composed of 3 or more amino acid sequences selectedfrom the group consisting of Ala, Val, Ile, Leu, and Pro;

(b) having proline as amino acid sequences corresponding to any one ormore of positions 5 to 8, and 12 of its amino acid sequence, andpreferably one or more of positions 5 to 8 and position 12 of its aminoacid sequence; and

(c) having an instability index of preferably 40-60 and more preferably41-58; an aliphatic index of preferably 180-220 and more preferably185-225; and a grand average of hydropathy (GRAVY) of preferably 2.1-2.6and more preferably 2.2-2.6 as measured by Protparam (seehttp://web.expasy.org/protparam/).

These critical factors that facilitate the cell permeable ability ofaMTD sequences were analyzed, identified, and determined according toone embodiment disclosed in the present application. These aMTDsequences are artificially assembled based on the critical factors (CFs)determined from in-depth analysis of previously published hydrophobicCPPs.

The aMTD sequences according to one aspect disclosed in the presentapplication are the first artificially developed cell permeablepolypeptides capable of mediating the transduction of biologicallyactive macromolecules—including peptides, polypeptides, protein domains,or full-length proteins—through the plasma membrane of cells.

According to one embodiment, one or more solubilization domain (SD)(s)are further fused to one or more of the SOCS3 protein and the aMTD,preferably one end or both ends of the SOCS3 protein, and morepreferably the C-terminus of the SOCS3 protein.

According to another embodiment, the aMTD may form α-Helix structure.

According to still another embodiment, the aMTD may be preferablycomposed of 12 amino acid sequences and represented by the followinggeneral formula:

Here, X(s) independently refer to Alanine (A), Valine (V), Leucine (L)or Isoleucine (I); and Proline (P) can be positioned in one of U(s)(either 5′, 6′, 7′ or 8′). The remaining U(s) are independently composedof A, V, L or I, P at the 12′ is Proline.

Still another aspect disclosed in the present application provides aniCP-SOCS3 recombinant protein which is represented by any one ofstructural formulae A-B-C, A-C-B, B-A-C, B-C-A, C-A-B, C-B-A andA-C-B-C, and preferably by A-B-C or C-B-A:

wherein A is an advanced macromolecule transduction domain (aMTD) havingimproved cell and/or tissue permeability, B is a SOCS3 protein, and C isa solubilization domain (SD); and

the aMTD is composed of 9-13, preferably 10-12 amino acid sequences andhas the following features of:

(a) being composed of 3 or more amino acid sequences selected from thegroup consisting of Ala, Val, Ile, Leu, and Pro;

(b) having proline as amino acid sequences corresponding to any one ormore of positions 5 to 8, and 12 of its amino acid sequence, andpreferably, one or more of positions 5 to 8 and position 12 of its aminoacid sequence;

(c) having an instability index of 40-60, preferably 41-58 and morepreferably 50-58; an aliphatic index of 180-220. preferably 185-225 andmore preferably 195-205; and a grand average of hydropathy (GRAVY) of2.1-2.6 and preferably 2.2-2.6, as measured by Protparam (seehttp://web.expasy.org/protparam/); and

(d) preferably forming α-Helix structure.

In one embodiment disclosed in the present application, the SOCS3protein may have an amino acid sequence of SEQ ID NO: 814.

In another embodiment disclosed in the present application, the SOCS3protein may be encoded by a polynucleotide sequence of SEQ ID NO: 815.

When the iCP-SOCS3 recombinant protein is intended to be delivered to aparticular cell, tissue, or organ, the SOCS3 protein may form a fusionproduct, together with an extracellular domain of a ligand capable ofselectively binding to a receptor which is specifically expressed on theparticular cell, tissue, or organ, or monoclonal antibody (mAb) capableof specifically binding to the receptor or the ligand and a modifiedform thereof.

The binding of the peptide and a biologically active substance may beformed either by indirect linkage by a cloning technique using anexpression vector at a nucleotide level or by direct linkage viachemical or physical covalent or non-covalent bond of the peptide andthe biologically active substance.

In still another embodiment disclosed in the present application, theSOCS3 protein may preferably further include a ligand selectivelybinding to a receptor of a cell, a tissue, or an organ.

In one embodiment disclosed in the present application, the aMTD mayhave an amino acid sequence selected from the group consisting of SEQ IDNOs: 1-240 and 822, preferably SEQ ID NOs: 2, 16, 22, 32, 40, 43, 63,65, 77, 84, 85, 86, 110, 131, 142, 143, 177, 228, 229, 233, 237, 239 and822, more preferably SEQ ID NO: 43.

In still another embodiment disclosed in the present application, theaMTD may be encoded by a polynucleotide sequence selected from the groupconsisting of SEQ ID NOs: 241-480 and 823, preferably SEQ ID NOs: 242,256, 262, 272, 280, 283, 303, 305, 317, 324, 325, 326, 350, 371, 382,383, 417, 468, 469 473, 477, 479 and 823, more preferably SEQ ID NO:283.

In still another embodiment disclosed in the present application, theSD(s) may have an amino acid sequence independently selected from thegroup consisting of SEQ ID NOs: 798, 799, 800, 801, 802, 803, and 804.The SD may be preferably SDA of SEQ ID NO: 798, SDB of SEQ ID NO: 799,or SDB′ of SEQ ID NO: 804, and more preferably, SDB of SEQ ID NO: 799which has superior structural stability, or SDB′ of SEQ ID NO: 804 whichhas a modified amino acid sequence of SDB to avoid immune responses uponin vivo application. The modification of the amino acid sequence in SDBmay be replacement of an amino acid residue, Valine, corresponding toposition 28 of the amino acid sequence of SDB (SEQ ID NO: 799) byLeucine.

In still another embodiment disclosed in the present application, theSDs may be encoded by a polynucleotide sequence independently selectedfrom the group consisting of SEQ ID NOs: 805, 806, 807, 808, 809, 810,and 811. The SD may be preferably SDA encoded by a polynucleotidesequence of SEQ ID NO: 805, SDB encoded by a polynucleotide sequence ofSEQ ID NO: 806, or SDB′ for deimmunization (or humanization) encoded bya polynucleotide sequence of SEQ ID NO: 811, and more preferably, SDBhaving superior structural stability, which is encoded by apolynucleotide sequence of SEQ ID NO: 806, or SDB′ having a modifiedpolynucleotide sequence of SDB to avoid immune responses upon in vivoapplication, which is encoded by a polynucleotide sequence of SEQ ID NO:811.

In still another embodiment disclosed in the present application, theiCP-SOCS3 recombinant protein may be preferably selected from the groupconsisting of:

1) a recombinant protein, in which SOCS3 having an amino acid sequenceof SEQ ID NO: 814 is fused to the N-terminus or the C-terminus of aMTDhaving any one amino acid sequence selected from the group consisting ofSEQ ID NOs: 1 to 240 and 822, 2, 16, 22, 32, 40, 43, 63, 65, 77, 84, 85,86, 110, 131, 142, 143, 177, 228, 229, 233, 237, 239 and 822, morepreferably SEQ ID NO: 43;

2) a recombinant protein, in which SD having any one amino acid sequenceselected from the group consisting of SEQ ID NOs: 798 to 804 is furtherfused to one or more of the N-terminus or the C-terminus of the SOCS3and aMTD in the recombinant protein of 1); and

3) a recombinant protein, in which a Histidine tag having an amino acidsequence of 812 is further fused to the N-terminus of the recombinantprotein of 1) or 2).

According to one embodiment, the iCP-SOCS3 recombinant protein may becomposed of an amino acid sequence selected from the group consisting ofSEQ ID NO: 825.

The SOCS3 protein may exhibit a physiological phenomenon-relatedactivity or a therapeutic purpose-related activity by intracellular orin-vivo delivery. The recombinant expression vector may include a tagsequence which makes it easy to purify the recombinant protein, forexample, consecutive histidine codon, maltose binding protein codon, Myccodon, etc., and further include a fusion partner to enhance solubilityof the recombinant protein, etc. Further, for the overall structural andfunctional stability of the recombinant protein or flexibility of theproteins encoded by respective genes, the recombinant expression vectormay further include one or more glycine, proline, and spacer amino acidor polynucleotide sequences including AAY amino acids. Furthermore, therecombinant expression vector may include a sequence specificallydigested by an enzyme in order to remove an unnecessary region of therecombinant protein, an expression regulatory sequence, and a marker orreporter gene sequence to verify intracellular delivery, but is notlimited thereto.

In still another embodiment disclosed in the present application, theiCP-SOCS3 recombinant protein may preferably have a histidine-tagaffinity domain additionally fused to one end thereof.

In still another embodiment disclosed in the present application, thehistidine-tag affinity domain may have an amino acid sequence of SEQ IDNO: 812.

In still another embodiment disclosed in the present application, thehistidine-tag affinity domain may be encoded by a polynucleotidesequence of SEQ ID NO: 813.

In still another embodiment disclosed in the present application, thefusion may be formed via a peptide bond or a chemical bond.

The chemical bond may be preferably selected from the group consistingof disulfide bonds, diamine bonds, sulfide-amine bonds, carboxyl-aminebonds, ester bonds, and covalent bonds.

In still another embodiment disclosed in the present application, theiCP-SOCS3 recombinant protein may be used for the treating, preventing,or delaying the onset of, solid tumor.

Still another aspect disclosed in the present application provides apolynucleotide sequence encoding the iCP-SOCS3.

According to still another embodiment disclosed in the presentapplication, the polynucleotide sequence may be fused with ahistidine-tag affinity domain.

Still another aspect disclosed in the present application provides arecombinant expression vector including the polynucleotide sequence.

Preferably, the vector may be inserted in a host cell and recombinedwith the host cell genome, or refers to any nucleic acid including anucleotide sequence competent to replicate spontaneously as an episome.Such a vector may include a linear nucleic acid, a plasmid, a phagemid,a cosmid, an RNA vector, a viral vector, etc.

Preferably, the vector may be genetically engineered to incorporate thenucleic acid sequence encoding the recombinant protein in an orientationeither N-terminal and/or C-terminal to a nucleic acid sequence encodinga peptide, a polypeptide, a protein domain, or a full-length protein ofinterest, and in the correct reading frame so that the recombinantprotein consisting of aMTD, SOCS3 protein, and preferably SD may beexpressed. Expression vectors may be selected from those readilyavailable for use in prokaryotic or eukaryotic expression systems.

Standard recombinant nucleic acid methods may be used to express agenetically engineered recombinant protein. The nucleic acid sequenceencoding the recombinant protein according to one embodiment disclosedin the present application may be cloned into a nucleic acid expressionvector, e.g., with appropriate signal and processing sequences andregulatory sequences for transcription and translation, and the proteinmay be synthesized using automated organic synthetic methods. Syntheticmethods of producing proteins are described in, for example, theliterature [Methods in Enzymology, Volume 289: Solid-Phase PeptideSynthesis by Gregg B. Fields (Editor), Sidney P. Colowick, Melvin I.Simon (Editor), Academic Press (1997)].

In order to obtain high level expression of a cloned gene or nucleicacid, for example, a cDNA encoding the recombinant protein according toone embodiment disclosed in the present application, the recombinantprotein sequence may be typically subcloned into an expression vectorthat includes a strong promoter for directing transcription, atranscription/translation terminator, and in the case of a nucleic acidencoding a protein, a ribosome binding site for translationalinitiation. Suitable bacterial promoters are well known in the art andare described, e.g., in the literatures [Sambrook & Russell, MolecularCloning: A Laboratory Manual, 3d Edition, Cold Spring Harbor Laboratory,N.Y. (2001); and Ausubel, et al., Current Protocols in MolecularBiology, Greene Publishing Associates and Wiley Interscience, N. Y.(1989)]. Bacterial expression systems for expression of the recombinantprotein are available in, e.g., E. coli, Bacillus sp., and Salmonella(Palva et al., Gene 22: 229-235 (1983); Mosbach et al., Nature 302:543-545 (1983)). Kits for such expression systems are commerciallyavailable. Eukaryotic expression systems for mammalian cells, yeast, andinsect cells are well known in the art and are also commerciallyavailable. The eukaryotic expression vector may be preferably anadenoviral vector, an adeno-associated vector, or a retroviral vector.

Generally, the expression vector for expressing the cell permeablerecombinant protein according to one embodiment disclosed in the presentapplication in which the cargo protein, i.e. ASOCS3 protein, is attachedto the N-terminus, C-terminus, or both termini of aMTD may includeregulatory sequences including, for example, a promoter, operablyattached to a sequence encoding the advanced macromolecule transductiondomain. Non-limiting examples of inducible promoters that may be usedinclude steroid-hormone responsive promoters (e.g., ecdysone-responsive,estrogen-responsive, and glutacorticoid-responsive promoters),tetracycline “Tet-On” and “Tet-Off” systems, and metal-responsivepromoters.

The polynucleotide sequence according to one embodiment disclosed in thepresent application may be present in a vector in which thepolynucleotide sequence is operably linked to regulatory sequencescapable of providing for the expression of the polynucleotide sequenceby a suitable host cell.

According to one embodiment disclosed in the present application, thepolynucleotide sequence may be selected from the following groups:

1) a polynucleotide sequence, in which any one polynucleotide sequenceselected from the group consisting of SEQ ID NOs: 241-480 and 823,preferably SEQ ID NOs: 242, 252, 274, 279, 322, 331, 338, 345, 347, 361,365, 370, 371, 383, 387, 417, 462, 468, 469, 473, 477, 479 and 823, morepreferably SEQ ID NO: 283, is operably linked with a polynucleotidesequence of SEQ ID NO: 815; and

2) a polynucleotide sequence, in which any one polynucleotide sequenceselected from the group consisting of SEQ ID NOs: 805 to 811 is furtheroperably linked to the polynucleotide sequence of 1), or furtheroperably linked to between: any one polynucleotide sequence selectedfrom the group consisting of SEQ ID NOs: 241-480 and 823, preferably SEQID NOs: 242, 256, 262, 272, 280, 283, 303, 305, 317, 324, 325, 326, 350,371, 382, 383, 417, 468, 469, 473, 477, 479 and 823, more preferably SEQID NO: 283; and a polynucleotide sequence of SEQ ID NO: 815.

Within an expression vector, the term “operably linked” is intended tomean that the polynucleotide sequence of interest is linked to theregulatory sequence(s) in a manner which allows for expression of thepolynucleotide sequence. The term “regulatory sequence” is intended toinclude promoters, enhancers, and other expression control elements.Such operable linkage with the expression vector can be achieved byconventional gene recombination techniques known in the art, whilesite-directed DNA cleavage and linkage are carried out by usingconventional enzymes known in the art.

The expression vectors may contain a signal sequence or a leadersequence for membrane targeting or secretion, as well as regulatorysequences such as a promoter, an operator, an initiation codon, atermination codon, a polyadenylation signal, an enhancer and the like.The promoter may be a constitutive or an inducible promoter. Further,the expression vector may include one or more selectable marker genesfor selecting the host cell containing the expression vector, and mayfurther include a polynucleotide sequence that enables the vector toreplicate in the host cell in question.

The expression vector constructed according to one embodiment disclosedin the present application may be the vector where the polynucleotideencoding the iCP-SOCS3 recombinant protein (where an aMTD is fused tothe N-terminus or C-terminus of a SOCS3 protein) is inserted within themultiple cloning sites (MCS), preferably within the Nde1/Sal1 site orBamH1/Sal1 site of a pET-28a(+)(Novagen, Darmstadt, Germany) orpET-26b(+) vector(Novagen, Darmstadt, Germany).

In still another embodiment disclosed in the present application, thepolynucleotide encoding the SD being additionally fused to theN-terminus or C-terminus of a SOCS3 protein or an aMTD may be insertedinto a cleavage site of restriction enzyme (Nde1, BamH1 and Sal1, etc.)within the multiple cloning sites (MCS) of a pET-28a(+)(Novagen,Darmstadt, Germany) or pET-26b(+) vector(Novagen, Darmstadt, Germany).

In still another embodiment disclosed in the present application, thepolynucleotide encoding the iCP-SOCS3 recombinant protein may be clonedinto a pET-28a(+) vector bearing a His-tag sequence so as to fuse sixhistidine residues to the N-terminus of the iCP-SOCS3 recombinantprotein to allow easy purification.

According to one embodiment disclosed in the present application, thepolynucleotide sequence may be a polynucleotide sequence selected fromthe group consisting of SEQ ID NOS: 824, 826, 828 and 830.

The recombinant protein may be introduced into an appropriate host cell,e.g., a bacterial cell, a yeast cell, an insect cell, or a tissueculture cell. The recombinant protein may also be introduced intoembryonic stem cells in order to generate a transgenic organism. Largenumbers of suitable vectors and promoters are known to those skilled inthe art and are commercially available for generating the recombinantprotein.

Known methods may be used to construct vectors including thepolynucleotide sequence according to one embodiment disclosed in thepresent application and appropriate transcriptional/translationalcontrol signals. These methods include in vitro recombinant DNAtechniques, synthetic techniques, and in vivo recombination/geneticrecombination. For example, these techniques are described in theliteratures [Sambrook & Russell, Molecular Cloning: A Laboratory Manual,3d Edition, Cold Spring Harbor Laboratory, N. Y. (2001); and Ausubel etal., Current Protocols in Molecular Biology Greene Publishing Associatesand Wiley Interscience, N.Y. (1989)].

Still another aspect disclosed in the present application provides atransformant transformed with the recombinant expression vector.

The transformation includes transfection, and refers to a processwhereby a foreign (extracellular) DNA, with or without an accompanyingmaterial, enters into a host cell. The “transfected cell” refers to acell into which the foreign DNA is introduced into the cell, and thusthe cell harbors the foreign DNA. The DNA may be introduced into thecell so that a nucleic acid thereof may be integrated into thechromosome or replicable as an extrachromosomal element. The cellintroduced with the foreign DNA, etc. is called a transformant.

As used herein, ‘introducing’ of a protein, a peptide, an organiccompound into a cell may be used interchangeably with the expression of‘carrying,’ ‘penetrating,’ ‘transporting,’ ‘delivering,’ ‘permeating’ or‘passing.’

It is understood that the host cell refers to a eukaryotic orprokaryotic cell into which one or more DNAs or vectors are introduced,and refers not only to the particular subject cell but also to theprogeny or potential progeny thereof. Because certain modifications mayoccur in succeeding generations due to either mutation or environmentalinfluences, such progeny may not, in fact, be identical to the parentcell, but are still included within the scope of the term as usedherein.

The host cells may be preferably bacterial cells, and as the bacterialcells, there are, in principle, no limitations. They may be eubacteria(gram-positive or gram-negative) or archaebacteria, as long as theyallow genetic manipulation for insertion of a gene of interest,preferably for site-specific integration, and they may be cultured on amanufacturing scale. Preferably, the host cells may have the property toallow cultivation to high cell densities.

Examples of bacterial host cells that may be used in the preparation ofthe recombinant protein are E. coli (Lee, 1996; Hannig and Makrides,1998), Bacillus subtilis, Pseudomonas fluorescens (Squires et al., 2004;Retallack et al., 2006) as well as various Corynebacterium (US2006/0003404 A1) and Lactococcus lactis (Mierau et al., 2005) strains.Preferably, the host cells are Escherichia coli cells.

More preferably, the host cell may include an RNA polymerase capable ofbinding to a promoter regulating the gene of interest. The RNApolymerase may be endogenous or exogenous to the host cell.

Preferably, host cells with a foreign strong RNA polymerase may be used.For example, Escherichia coli strains engineered to carry a foreign RNApolymerase (e.g. like in the case of using a T7 promoter a T7-like RNApolymerase in the so-called “T7 strains”) integrated in their genome maybe used. Examples of T7 strains, e.g. BL21(DE3), HMS174(DE3), and theirderivatives or relatives (see Novagen, pET System manual, 11^(th)edition), may be widely used and commercially available. Preferably,BL21-CodonPlus (DE3)-RIL or BL21-CodonPlus (DE3)-RIPL (AgilentTechnologies) may be used. These strains are DE3 lysogens containing theT7 RNA polymerase gene under control of the lacUV5 promoter. Inductionwith IPTG allows production of T7 RNA polymerase which then directs theexpression of the gene of interest under the control of the T7 promoter.

The host cell strains, E. coli BL21(DE3) or HMS174(DE3), which havereceived their genome-based T7 RNA polymerase via the phage DE3, arelysogenic. It is preferred that the T7 RNA polymerase contained in thehost cell has been integrated by a method which avoids, or preferablyexcludes, the insertion of residual phage sequences in the host cellgenome since lysogenic strains have the disadvantage to potentiallyexhibit lytic properties, leading to undesirable phage release and celllysis.

Still another aspect disclosed in the present application provides apreparing method of the iCP-SOCS3 recombinant protein includingpreparing the recombinant expression vector; preparing the transformantusing the recombinant expression vector; culturing the transformant; andrecovering the recombinant protein expressed by culturing.

Culturing may be preferably in a mode that employs the addition of afeed medium, this mode being selected from the fed-batch mode,semi-continuous mode, or continuous mode, and the bacterial expressionhost cells may include a DNA construct, integrated in their genome,carrying the DNA sequence encoding the protein of interest under thecontrol of a promoter that enables expression of said protein.

There are no limitations in the type of the culture medium. The culturemedium may be semi-defined, i.e. containing complex media compounds(e.g. yeast extract, soy peptone, casamino acids), or it may bechemically defined, without any complex compounds. Preferably, a definedmedium may be used. The defined media (also called minimal or syntheticmedia) are exclusively composed of chemically defined substances, i.e.carbon sources such as glucose or glycerol, salts, vitamins, and, inview of a possible strain auxotrophy, specific amino acids or othersubstances such as thiamine. Most preferably, glucose may be used as acarbon source. Usually, the carbon source of the feed medium serves asthe growth-limiting component which controls the specific growth rate.

Host cells may be disrupted by any convenient method, includingfreeze-thaw cycling, sonication, mechanical disruption, or the use ofcell lysing agents. The literature [Scopes, Protein Purification:Principles and Practice, New York: Springer-Verlag (1994)] describes anumber of general methods for purifying recombinant (andnon-recombinant) proteins. The methods may include, e.g., ion-exchangechromatography, size-exclusion chromatography, affinity chromatography,selective precipitation, dialysis, and hydrophobic interactionchromatography. These methods may be adapted to devise a purificationstrategy for the cell permeable recombinant protein. If the cellpermeable recombinant protein includes a purification handle, such as anepitope tag or a metal chelating sequence, affinity chromatography maybe used to easily purify the protein.

The amount of the protein produced may be evaluated by detecting theadvanced macromolecule transduction domain directly (e.g., using Westernanalysis) or indirectly (e.g., by assaying materials derived from thecells for specific DNA binding activity, such as by electrophoreticmobility shift assay). Proteins may be detected prior to purification,during any stage of purification, or after purification. In someimplementations, purification or complete purification may not benecessary.

The iCP-SOCS3 recombinant proteins according to one embodiment disclosedin the present application are cell permeable proteins, and may be usedas protein-based vaccines, particularly in the case where killed orattenuated whole organism vaccines are impractical.

The iCP-SOCS3 recombinant proteins according to one embodiment disclosedin the present application may be preferably used for the treating,preventing, or delaying the onset of, solid tumor. The cell permeablerecombinant proteins may be delivered to the interior of the cell,eliminating the need to transfect or transform the cell with arecombinant vector. The cell permeable recombinant proteins may be usedin vitro to investigate protein function or may be used to maintaincells in a desired state.

Still another aspect disclosed in the present application provides acomposition including the iCP-SOCS3 Recombinant Protein as an activeingredient.

Still another aspect disclosed in the present application provides apharmaceutical composition for treating, preventing, or delaying theonset of, solid tumor including the iCP-SOCS3 Recombinant Protein as anactive ingredient; and a pharmaceutically acceptable carrier.

According to one embodiment disclosed in the present application, theiCP-SOCS3 Recombinant Protein may be used in a single agent, or incombination with one or more other anti-cancer agents.

Solid tumor described herein include, but are not limited to, describedherein include, but are not limited to, prostate cancer, renal cancer,hepatic cancer, colon cancer, rectal cancer, colorectal cancer, braincancer, renal cancer, colorectal cancer, pancreatic cancer, gastriccancer, breast cancer, lung cancer, cancers of the head and neck,thyroid cancer, glioblastoma, Kaposi's sarcoma, melanoma, oesophagealcancer, gastro-oesophageal cancer, cervical cancer, hepatocellularcarcinoma, endometrial cancer, urothelial cancer, or ovarian cancer.

According to one embodiment disclosed in the present invention, thetumor may be early stage tumor, metastatic tumor, non-metastatic tumor,primary tumor, resected tumor, advanced tumor, locally advanced tumor,unresectable tumor, tumor in remission, or recurrent tumor.

Preferably, the composition may be for injectable (e.g. intraperitoneal,intravenous, and intra-arterial, etc.) and may include the activeingredient in an amount of 0.001 mg/kg to 1000 mg/kg, preferably 0.01mg/kg to 100 mg/kg, more preferably 0.1 mg/kg to 50 mg/kg for human.

For examples, dosages per day normally fall within the range of about0.001 to about 1000 mg/kg of body weight. In the treatment of adulthumans, the range of about 0.1 to about 50 mg/kg/day, in single ordivided dose, is especially preferred. However, it will be understoodthat the concentration of the iCP-SOCS3 recombinant protein actuallyadministered will be determined by a physician, in the light of therelevant circumstances, including the condition to be treated, thechosen route of administration, the age, weight, and response of theindividual patient, and the severity of the patient's symptoms, andtherefore the above dosage ranges are not intended to limit the scope ofthe invention in any way. In some instances dosage levels below thelower limit of the aforesaid range may be more than adequate, while inother cases still larger doses may be employed without causing anyharmful side effect, provided that such larger doses are first dividedinto several smaller doses for administration throughout the day.

Still another aspect disclosed in the present application provides useof the iCP-SOCS3 recombinant protein as a medicament for treating,preventing, or delaying the onset of, solid tumor.

Still another aspect disclosed in the present application provides amedicament including the iCP-SOCS3 recombinant protein.

Still another aspect disclosed in the present application provides useof the iCP-SOCS3 recombinant protein for the preparation of a medicamentfor treating, preventing, or delaying the onset of, solid tumor.

Still another aspect disclosed in the present application provides amethod of treating, preventing, or delaying the onset of, solid tumor ina subject including identifying a subject in need of treating,preventing, or delaying the onset of, solid tumor; and administering tothe subject a therapeutically effective amount of the iCP-SOCS3recombinant protein.

In one embodiment disclosed in the present application, the subject maybe preferably a mammal.

Preferably, the subject in need of treating, preventing, or delaying theonset of, solid tumor may be identified by any conventional diagnosticmethods known in the art including ultrasound, CT scan, MRI,alpha-fetoprotein testing, and biopsy, etc.

The pharmaceutical composition according to one embodiment disloced inthe present application may be prepared by using pharmaceuticallysuitable and physiologically acceptable additives, in addition to theactive ingredient, and the additives may include excipients,disintegrants, sweeteners, binders, coating agents, blowing agents,lubricants, glidants, flavoring agents, etc.

For administration, the pharmaceutical composition may be preferablyformulated by further including one or more pharmaceutically acceptablecarriers in addition to the above-described active ingredient.

Dosage forms of the pharmaceutical composition may include granules,powders, tablets, coated tablets, capsules, suppositories, liquidformulations, syrups, juice, suspensions, emulsions, drops, injectableliquid formulations, etc. For formulation of the composition into atablet or capsule, for example, the active ingredient may be combinedwith any oral, non-toxic pharmaceutically acceptable inert carrier, suchas ethanol, glycerol, water, etc. If desired or necessary, suitablebinders, lubricants, disintegrants, and colorants may be additionallyincluded as a mixture.

Examples of the suitable binder may include, but are not limited to,starch, gelatin, natural sugars such as glucose or beta-lactose, cornsweeteners, natural and synthetic gums such as acacia, tragacanth, orsodium oleate, sodium stearate, magnesium stearate, sodium benzoate,sodium acetate, sodium chloride, etc. Examples of the disintegrant mayinclude, but are not limited to, starch, methyl cellulose, agar,bentonite, xanthan gum, etc. For formulation of the composition into aliquid preparation, a pharmaceutically acceptable carrier which issterile and biocompatible may be used, such as saline, sterile water, aRinger's solution, buffered saline, an albumin infusion solution, adextrose solution, a maltodextrin solution, glycerol, and ethanol, andthese materials may be used alone or in any combination thereof. Ifnecessary, other common additives, such as antioxidants, buffers,bacteriostatic agents, etc., may be added. Further, diluents,dispersants, surfactants, binders, and lubricants may be additionallyadded to prepare injectable formulations such as aqueous solutions,suspensions, and emulsions, or pills, capsules, granules, or tablets.Furthermore, the composition may be preferably formulated, dependingupon diseases and ingredients, using any appropriate method known in theart, as disclosed in Remington's Pharmaceutical Science, Mack PublishingCompany, Easton Pa.

Preferably, the treatment or treating mean improving or stabilizing thesubject's condition or disease; or preventing or relieving thedevelopment or worsening of symptoms associated with the subject'scondition or disease. The prevention, prophylaxis and preventivetreatment are used herein as synonyms.

Preferably, the treating, preventing, or delaying the onset of, solidtumor may be any one or more of the following: alleviating one or moresymptoms of tumor, delaying progressing of tumor, shrinking tumor sizein patient, inhibiting tumor growth, prolonging overall survival,prolonging disease-free survival, prolonging time to diseaseprogression, preventing or delaying tumor metastasis, reducing oreradiating preexisting tumor metastasis, reducing incidence or burden ofpreexisting tumor metastasis, preventing recurrence of tumor.

The subject and patient are used herein interchangeably. They refer to ahuman or another mammal (e.g., mouse, rat, rabbit, dog, cat, cattle,swine, sheep, horse or primate) that can be afflicted with or issusceptible to a disease or disorder but may or may not have the diseaseor disorder. In certain embodiments, the subject is a human being.

Preferably, the amount effective or effective amount is the amount of anactive ingredient or a pharmaceutical composition disclosed herein thatwhen administered to a subject for treating a disease, is sufficient toeffect such treatment of the disease. Any improvement in the patient isconsidered sufficient to achieve treatment. An effective amount of anactive ingredient or a pharmaceutical composition disclosed herein, usedfor the preventing, or delaying the onset of, solid tumor may varydepending upon the manner of administration, the age, body weight, andgeneral health of the patient. Ultimately, the prescribers orresearchers will decide the appropriate amount and dosage regimen.

In the treatment or prevention method according to one embodimentdisclosed in the present application, the composition including theiCP-SOCS3 recombinant protein as an active ingredient may beadministered in a common manner via oral, buccal, rectal, intravenous,intra-arterial, intraperitoneal, intramuscular, intrasternal,percutaneous, topical, intraocular or subcutaneous route, morepreferably via intraperitoneal, intravenous, or intra-arterial injectionroute.

Advantageous Effects

According to one aspect disclosed in the present application,development and establishment of improved cell-permeable SOCS3recombinant protein, as therapeutics of solid tumor are provided.Because iCP-SOCS3 was designed based on endogenous proteins, it would bea safety anti-solid tumor drug without side-effect.

However, the effects of the disclosures in the present application arenot limited to the above-mentioned effects, and another effects notmentioned will be clearly understood by those skilled in the art fromthe following description.

DESCRIPTION OF DRAWINGS

FIG. 1 shows Structure of aMTD- or rPeptide-Fused Recombinant Proteins.A schematic diagram of the His-tagged CRA recombinant proteins isillustrated and constructed according to the present invention. Thehis-tag for affinity purification (white), aMTD or rPeptide (gray) andcargo A (CRA, black) are shown.

FIG. 2a shows Construction of Expression Vectors for aMTDs- orrPeptide-Fused Recombinant Proteins. FIGS. 2b and 2c show the agarosegel electrophoresis analysis showing plasmid DNA fragments at 645 bpinsert encoding aMTDs or rPeptide-fused CRA cloned into the pET28a(+)vector according to the present invention.

FIGS. 3a to 3d show Inducible Expression of aMTD- or rPeptide-FusedRecombinant Proteins. Expressed recombinant aMTD- or randompeptide-fused CRA recombinant proteins were transformed in E. coli BL21(DE3) strain. Expression of recombinant proteins in E. coli before (−)and after (+) induction with IPTG was monitored by SDS-PAGE, and stainedwith Coomassie blue.

FIGS. 4a and 4b show Purification of aMTD- or rPeptide-Fused RecombinantProteins. Expressed recombinant proteins were purified by Ni²+affinitychromatography under the natural condition. Purification of recombinantproteins displayed through SDS-PAGE analysis.

FIGS. 5a to 5u show Determination of aMTD-Mediated Cell-Permeability.Cell-permeability of a negative control (A: rP38) and referencehydrophobic CPPs (MTM12 and MTD85) are shown. The cell-permeability ofeach aMTD and/or rPeptide is visually compared to that of the cargoprotein lacking peptide sequence (HCA). Gray shaded area representsuntreated RAW 264.7 cells (vehicle); thin light gray line represents thecells treated with equal molar concentration of FITC (FITC only); darkthick line indicates the cells treated with FITC-his-tagged CRA protein(HCA); and the cells treated with the FITC-proteins (HMCA) fused tonegative control (rP38), reference CPP (MTM12 or MTD85) or newhydrophobic CPP (aMTD) are shown with light thick line and indicated byarrows.

FIGS. 6a to 6c show Determination of rPeptide-MediatedCell-Permeability. The cell-permeability of each aMTD and/or rPeptidewas visually compared to that of the cargo protein lacking peptidesequence (HCA). Gray shaded area represents untreated RAW 264.7 cells(vehicle); thin light gray line represents the cells treated with equalmolar concentration of FITC (FITC only); dark thick line indicates thecells treated with FITC-his-tagged CRA protein (HCA); and the cellstreated with the FITC-proteins fused to rPeptides are shown with lightthick line and indicated by arrows.

FIGS. 7a to 7k shows Visualized Cell-Permeability of aMTD-FusedRecombinant Proteins. NIH3T3 clls were treated with FITC-labeled protein(10 μM) fused to aMTD for 1 hour at 37. Cell-permeability of theproteins was visualized by laser scanning confocal microscopy (LSM700version).

FIG. 8 shows Visualized Cell-Permeability of rPeptide-Fused RecombinantProteins. Cell-permeability of rPeptide-fused recombinant proteins wasvisualized by laser scanning confocal microscopy (LSM700 version).

FIGS. 9a to 9c show Relative Cell-Permeability of aMTD-Fused RecombinantProteins Compared to Negative Control (rP38). The FIG shows graphscomparing the cell-permeability of the recombinant proteins fused toaMTDs and a negative control (A: rP38).

FIGS. 10a to 10c show Relative Cell-Permeability of aMTD-FusedRecombinant Proteins Compared to Reference CPP (MTM12). The FIG showsgraphs comparing the cell-permeability of the recombinant proteins fusedto aMTDs and a reference CPP (MTM12).

FIGS. 11a to 11c show Relative Cell-Permeability of aMTD-FusedRecombinant Proteins Compared to Reference CPP (MTD85). The FIG showsgraphs comparing the cell-permeability of the recombinant proteins fusedto aMTDs and a reference CPP (MTD85).

FIG. 12 shows Relative Cell-Permeability of rPeptide-MediatedRecombinant Proteins Compared to Average that of aMTDs. The FIG showsgraphs comparing the cell-permeability of the recombinant proteins fusedto rPeptides and that (average value: aMTD AVE) of aMTDs.

FIGS. 13a and 13b show Association of Cell-Permeability with Amino AcidComposition in aMTD Sequences. These graphs display delivery potential(Geometric Mean) of aMTDs influenced with amino acid composition (A, I,V and L).

FIGS. 14a and 14b show Association of Cell-Permeability with CriticalFactors in aMTDs. These graphs show the association of cell-permeabilitywith critical factors [bending potential: proline position (PP),rigidity/flexibility: instability index (II), structural feature:aliphatic index (AI) and hydropathy: grand average of hydropathy(GRAVY)].

FIGS. 15a and 15b show Relative Relevance of aMTD-MediatedCell-Permeability with Critical Factors. Cell-permeability of 10 highand 10 low ranked aMTDs in their delivery potential were examined fortheir association with the critical factors [bending potential: prolineposition (PP), rigidity/flexibility: instability index (II), structuralfeature: aliphatic index (AI) and hydropathy: grand average ofhydropathy (GRAVY)].

FIG. 16 shows Relative Relevance of rPeptide-Mediated Cell-Permeabilitywith Hydropathy Range (GRAVY). This graph and a chart illustraterelative relevance of rPeptide-mediated cell-permeability with itshydropathy range (GRAVY).

FIG. 17 shows a structure of iCP-SOCS3 recombinant protein designedaccording to example 6-1.

FIG. 18 shows the agarose gel electrophoresis analysis showing plasmidDNA fragments insert encoding His-SOCS3-SDB (HS3B),His-aMTD₁₆₅-SOCS3-SDB (HM₁₆₅S3B), His-aMTD₁₆₅-SOC53-SDC (HM₁₆₅S3C),His-aMTD₁₆₅-SOC53-SDD (HM₁₆₅S3D), His-aMTD₁₆₅-SOC53-SDE (HM₁₆₅S3E)cloned into the pET28a (+) vector according to example 6-1.

FIG. 19 shows inducible expression and purification of iCP-SOCS3recombinant protein in E. coli according to example 6-2 and improvementof solubility/yield of iCP-SOCS3 recombinant protein by fusing aMTD/SDaccording to example 6-3.

FIG. 20 shows aMTD-Mediated cell-permeability of SOCS3 recombinantproteins in RAW 264.7 cells according to example 7-1.

FIG. 21 shows aMTD-Mediated intracellular delivery and localization ofSOCS3 Recombinant Proteins in NIH3T3 cells cells according to example7-1.

FIG. 22 shows systemic delivery of aMTD/SD-fused SOCS3 recombinantproteins in vivo according to example 7-2.

FIG. 23 shows inhibition of IFN-γ-induced STAT phosphorylation byiCP-SOCS3 recombinant protein according to example 8-1.

FIG. 24 shows inhibition of LPS-induced cytokines secretion by iCP-SOCS3recombinant protein according to example 8-2.

FIG. 25 shows the structures of SOCS3 recombinant protein lacking aMTDprepared as a negative control according to example 9.

FIG. 26 shows expression, purification, and solubility/yield of HS3(lacking aMTD and SD) and HS3B (lacking aMTD) determined according toexample 6-3.

FIG. 27 shows the agarose gel electrophoresis analysis showing plasmidDNA fragments insert encoding His-aMTD#-SOCS3-SDB (HM_(#)S3B) andHis-rP#-SOCS3-SDB cloned into the pET28a (+) vector according to example10.

FIGS. 28a and 28b show expression, purification, and solubility/yield ofHis-aMTD#-SOCS3-SDB (HM_(#)S3B) determined according to example 10.

FIG. 29 shows expression, purification, and solubility/yield ofHis-rP#-SOCS3-SDB (HrP_(#)S3B) determined according to example 10.

FIG. 30 shows solubility/yield of His-aMTD#-SOCS3-SDB (HM_(#)SDB)determined according to example 10.

FIGS. 31 show aMTD-mediated cell-permeability. The cell-permeability ofeach SOCS3 recombinant protein fused with SD and various aMTD isvisually compared to that of the cargo protein lacking CPP (HS3B) orlacking CPP and SD (HS3). Gray shaded area represents untreated E. colicells (diluent); green line represents the cells treated with equalmolar concentration of FITC (FITC only); black line indicates the cellstreated with FITC-his-SOCS protein (FITC-HS3); blue line indicates thecells treated with FITC-his-SOCS-SDB protein (FITC-HS3B) purple lineindicates the cells treated with FITC-his-aMTD_(#)-SOCS-SDB protein(FITC-HM_(#)S3B).

FIG. 32 shows relative cell-permeability of His-aMTD_(#)-SOCS3-SDB-Fusedrecombinant proteins Compared to control (Vehicle, FITC only, HS3 andHS3B).

FIG. 33 shows random Peptide-Mediated cell-permeability. Thecell-permeability of each SOCS3 recombinant protein fused with SDB andaMTD₁₆₅ or various rP is visually compared to that of the cargo proteinlacking CPP (HS3B) or lacking CPP and SD (HS3). Gray shaded arearepresents untreated E. coli cells (diluent); green line represents thecells treated with equal molar concentration of FITC (FITC only); blackline indicates the cells treated with FITC-his-SOCS protein (FITC-HS3);blue line indicates the cells treated with FITC-his-SOCS-SDB protein(FITC-HS3B) and purple line indicates the cells treated withFITC-his-rPeptide_(#)-SOCS-SDB protein (FITC-HrP_(#)S3B).

FIG. 34 shows relative cell-permeability of His-rP_(#)-SOCS3-SDB-Fusedrecombinant proteins Compared to control (Vehicle, FITC only, HS3 andHS3B).

FIG. 35 shows apoptotic cells analysis according to example 11-1.

FIG. 36 shows induction of apoptosis by iCP-SOCS3 recombinant proteinsaccording to example 11-2.

FIG. 37 shows cell migration inhibition by iCP-SOCS3 recombinant proteinaccording to example 11-3.

FIG. 38 shows solubility/yield, permeability and biological activity ofHis-aMTD#-SOCS3-SDB (HM_(#)S3B) determined according to example 10 to11-3.

FIG. 39 shows expression, purification, and solubility/yield of M₁₆₅S3SB(lacking his-tag) determined according to example 12-1.

FIG. 40 shows cell-permeability of SOCS3 recombinant proteins (lackinghis-tag) in RAW 264.7 cells according to example 12-2.

FIG. 41 shows Annexin V analysis according to example 12-3.

FIG. 42 shows cell migration inhibition (bottom) by iCP-SOCS3recombinant protein according to example 12-3.

FIG. 43 shows a structure of iCP-SOCS3 recombinant protein(His-aMTD₁₆₅-SOCS3-SDB′) constructed according to example 12-4.

FIG. 44 shows expression, purification, and solubility/yield ofHM₁₆₅S3SB and HM₁₆₅S3SB′ determined according to example 12-4.

FIG. 45 shows aMTD-Mediated cell-permeability of iCP-SOCS3 recombinantproteins (HM₁₆₅S3B and HM₁₆₅S3B′(V28L)) in RAW 264.7 cells according toexample 12-5.

FIG. 46 shows antiproliferative activity of iCP-SOCS3 recombinantproteins (HM₁₆₅S3B and HM₁₆₅S3B′(V28L)) according to example 12-6.

FIG. 47 shows induction of apoptosis by iCP-SOCS3 recombinant proteins(HM₁₆₅S3B and HM₁₆₅S3B′(V28L)) according to example 12-6.

FIG. 48 shows cell migration inhibition by SOCS3 recombinant proteins(HM₁₆₅S3B and HM₁₆₅S3B′(V28L)) according to example 12-6.

FIG. 49 a shows a structure of iCP-SOCS3 recombinant proteins (top) andagarose gel electrophoresis analysis (bottom) according to example 12-7and FIG. 49 b shows inducible expressions and purifications of iCP-SOCS3recombinant protein in E. coli (bottom) according to example 12-7.

FIG. 50 shows inhibition of IFN-γ-induced STAT phosphorylation byiCP-SOCS3 recombinant protein according to example 13.

FIG. 51 shows effect of treating EDTA (FIG. 51A) and proteinase K (FIG.51B) on aMTD-mediated SOCS3 protein uptake into cells according toexample 14.

FIG. 52 shows effect of treating taxol (FIG. 52 A) and antimycin (FIG.52 B) on aMTD-mediated SOCS3 protein uptake into cells according toexample 14.

FIG. 53 shows effect of temperature on aMTD-mediated SOCS3 proteinuptake into cells according to example 14.

FIG. 54 shows aMTD-mediated cell-to-cell delivery assessed according toexample 14.

FIG. 55 shows bioavailability of iCP-SOCS3 recombinant protein in PBMC,splenocytes and hepatocytes analyzed by fluorescence microscopyaccording to example 15.

FIG. 56 shows biodistribution of iCP-SOCS3 recombinant protein inpancreas tissues analyzed by confocal microscope according to example15.

FIG. 57 shows aMTD-Mediated cell-permeability of SOCS3 recombinantproteins in various cancer cells according to example 16.

FIG. 58 shows systemic delivery of aMTD/SD-fused SOCS3 recombinantproteins into Various Tissues according to example 16.

FIG. 59 shows expression level of endogenous SOCS3 mRNA in gastric cellline analyzed by the agarose gel electrophoresis according to example17-1-2.

FIG. 60 shows expression level of SOCS3 gene, and phosphorylation ofJAK1 and JAK2 in gastric cancer cell line analyzed by western blotanalysis according to example 17-1-3.

FIG. 61 shows expression level of endogenous SOCS3 mRNA in colon cancercell line analyzed by the agarose gel electrophoresis according toexample 17-2-2.

FIG. 62 shows expression level of SOCS3 gene, and phosphorylation ofJAK1 and JAK2 in colon cancer cell line analyzed by western blotanalysis according to example 17-2-3.

FIG. 63 shows methylation and unmethylation level of endogenous SOCS3 inglioblastoma cell line analyzed by the agarose gel electrophoresisaccording to example 17-3-1.

FIG. 64 shows expression level of SOCS3 gene, and phosphorylation ofJAK1 and JAK2 in glioblastoma cancer cell line analyzed by western blotanalysis according to example 17-3-2.

FIG. 65 shows expression level of endogenous SOCS3 mRNA in breast cancercell line analyzed by the agarose gel electrophoresis according toexample 17-4-1.

FIG. 66 shows methylation and unmethylation level of endogenous SOCS3 inbreast cancer cell line analyzed by the agarose gel electrophoresisaccording to example 17-4-2.

FIG. 67 shows expression level of SOCS3 gene, and phosphorylation ofJAK1 and JAK2 in breast cancer cell line analyzed by western blotanalysis according to example 17-4-3.

FIG. 68 shows antiproliferative activity of iCP-SOCS3 recombinantprotein in gastric cancer cell line according to example 18-1.

FIG. 69 shows antiproliferative activity of iCP-SOCS3 recombinantprotein in colorectal cancer cell line according to example 18-1.

FIG. 70 shows antiproliferative activity of iCP-SOCS3 recombinantprotein in glioblastoma cell line according to example 18-1.

FIG. 71 shows antiproliferative activity of iCP-SOCS3 recombinantprotein in breast cancer cell line according to example 18-1.

FIG. 72 shows cell migration inhibition activity by iCP-SOCS3recombinant protein in gastric cancer cell line (AGS) according toexample 18-2.

FIG. 73 shows cell migration inhibition activity by iCP-SOCS3recombinant protein in colorectal cancer cell line (HCT116) according toexample 18-2.

FIG. 74 shows cell migration inhibition activity by iCP-SOCS3recombinant protein in glioblastoma cell line (U-87 MG) according toexample 18-2.

FIG. 75 shows cell migration inhibition activity by iCP-SOCS3recombinant protein in breast cancer cell line (MDA-MB-231) according toexample 18-2.

FIG. 76 show transwell migration inhibition activity by iCP-SOCS3recombinant protein in gastric cancer cell line (AGS) (FIG. 76, left),and decrease in invasion by iCP-SOCS3 recombinant protein in gastriccancer cell line (AGS) (FIG. 76, right) analyzed according to example18-2.

FIG. 77 show transwell migration inhibition activity by iCP-SOCS3recombinant protein in glioblstoma cell line (U-87 MG) analyzedaccording to example 18-2.

FIGS. 78 to 82 show induction of apoptosis in normal cells (FIG. 78), ingastric cancer cells (FIG. 79), in colorectal cancer cells (HCT116, FIG.80), in glioblastoma cells (U-87 MG, FIG. 81), and in breast cancercells (MDA-MB-231, FIG. 82) by iCP-SOCS3 recombinant proteins assessedby Annexin V staining according to example 18-3.

FIG. 83 show induction of apoptosis in colon cancer cells (HCT116) byiCP-SOCS3 recombinant proteins analyzed by TUNEL assay according toexample 18-4.

FIGS. 84 to 88 show inhibition of cell cycle progression in normal cells(FIG. 84), in gastric cancer cells (AGS, FIG. 85), in colon cancer cells(HCT116, FIG. 86), in glioblastoma cells (U-87 MG, FIG. 87), and inbreast cancer cells (MDA-MB-231, FIG. 88) by iCP-SOCS3 recombinantproteins assessed by flow cytometric analysis according to example 18-5.

FIG. 89 shows down regulation of expression of apoptosis by iCP-SOCS3recombinant proteins in breast cancer cells (MDA-MB-231) determined byimmunoblotting according to example 18-6.

FIG. 90 shows suppression of the tumor growth by iCP-SOCS3 recombinantproteins in gastric cancer cells (NCI-N87) assessed according to example19-1.

FIG. 91 shows suppression of the tumor growth by iCP-SOCS3 recombinantproteins in colorectal cancer cells (HCT116) assessed according toexample 19-2.

FIG. 92 shows suppression of the tumor growth by iCP-SOCS3 recombinantproteins in glioblastoma cells (U-87 MG) assessed according to example19-3.

FIG. 93 shows suppression of the tumor growth by iCP-SOCS3 recombinantproteins in breast cancer cells (MDA-MB-231) assessed according toexample 19-4.

FIG. 94 shows humanized SDB domain according to example 12-4.

FIG. 95 shows methylation and unmethylation level of endogenous SOCS3 ingastric cancer cell line analyzed by the agarose gel electrophoresisaccording to example 17-1-1.

FIG. 96 shows methylation and unmethylation level of endogenous SOCS3 incolon cancer cell line analyzed by the agarose gel electrophoresisaccording to example 17-2-1.

FIG. 97 shows sequences of amino acid and nucleotide of basic CPP, andprimers used in example 6-4.

FIG. 98 shows structure, expression, purification and solubility/yieldof aMTD/SD-fused SOCS3 recombinant protein and basic CPP/SD-fused SOCS3recombinant protein according to example 6-4.

FIG. 99 shows comparison of cell-permeability between aMTD/SD fusedSOCS3 recombinant proteins and basic/SD-fused in RAW 264.7 cellsaccording to example 7-1-2.

FIG. 100 shows comparison of tissue-permeability between aMTD/SD fusedSOCS3 recombinant proteins and basic/SD-fused in various tissues of ICRmice according to example 7-2-2.

FIG. 101 shows effect of treating protein K (FIG. 101 A) and Taxol (FIG.101 B) on aMTD (or basic CPP)-mediated SOCS3 protein uptake into cellsaccording to example 14-2.

FIGS. 102 and 103 show aMTD (or basic CPP)-mediated cell-to-celldelivery (FIG. 102) and cell-to-cell function assessed according toexample 14-2.

MODE FOR INVENTION 1. Analysis of Reference Hydrophobic CPPs to Identify‘Critical Factors’ for Development of Advanced MTDs

Previously reported MTDs were selected from a screen of more than 1,500signal peptide sequences. Although the MTDs that have been developed didnot have a common sequence or sequence motif, they were all derived fromthe hydrophobic (H) regions of signal sequences (HRSSs) that also lackcommon sequences or motifs except their hydrophobicity and the tendencyto adopt alpha-helical conformations. The wide variation in H-regionsequences may reflect prior evolution for proteins with membranetranslocating activity and subsequent adaptation to the SRP/Sec61machinery, which utilizes a methionine-rich signal peptide bindingpocket in SRP to accommodate a wide-variety of signal peptide sequences.

Previously described hydrophobic CPPs (e.g. MTS/MTM and MTD) werederived from the hydrophobic regions present in the signal peptides ofsecreted and cell surface proteins. The prior art consists first, of adhoc use of H-region sequences (MTS/MTM), and second, of H-regionsequences (with and without modification) with highest CPP activityselected from a screen of 1,500 signal sequences (MTM). Second priorart, the modified H-region derived hydrophobic CPP sequences hadadvanced in diversity with multiple number of available sequences apartfrom MTS/MTM derived from fibroblast growth factor (FGF) 4. However, thenumber of MTDs that could be modified from naturally occurring secretedproteins are somewhat limited. Because there is no set of rules indetermining their cell-permeability, no prediction for thecell-permeability of modified MTD sequences can be made before testingthem.

The hydrophobic CPPs, like the signal peptides from which theyoriginated, did not conform to a consensus sequence, and they hadadverse effects on protein solubility when incorporated into proteincargo. We therefore set out to identify optimal sequence and structuraldeterminants, namely critical factors (CFs), to design new hydrophobicCPPs with enhanced ability to deliver macromolecule cargoes includingproteins into the cells and tissues while maintaining proteinsolubility. These newly developed CPPs, advanced macromoleculetransduction domains (aMTDs) allowed almost infinite number of possibledesigns that could be designed and developed based on the criticalfactors. Also, their cell-permeability could be predicted by theircharacter analysis before conducting any in vitro and/or in vivoexperiments. These critical factors below have been developed byanalyzing all published reference hydrophobic CPPs.

1-1. Analysis of Hydrophobic CPPs

Seventeen different hydrophobic CPPs (Table 1) published from 1995 to2014 (Table 2) were selected. After physiological and chemicalproperties of selected hydrophobic CPPs were analyzed, 11 differentcharacteristics that may be associated with cell-permeability have beenchosen for further analysis. These 11 characteristics are as follows:sequence, amino acid length, molecular weight, pI value, bendingpotential, rigidity/flexibility, structural feature, hydropathy, residuestructure, amino acid composition and secondary structure of thesequences (Table 3).

Table 1 shows the summary of published hydrophobic Cell-PenetratingPeptides which were chosen.

TABLE 1 # Peptide Origin Protein Ref. 1 MTM Homo sapiens NP_001998Kaposi fibroblast growth factor (K-FGF) 1 2 MTS Homo sapiens NP_001998Kaposi fibroblast growth factor (K-FGF) 2 3 MTD10 Streptomycescoelicolor NP_625021 Glycosyl hydrolase 8 4 MTD13 Streptomycescoelicolor NP_639877 Putative secreted protein 5 5 MTD47 Streptomycescoelicolor NP_627512 Secreted protein 7 6 MTD56 Homo sapiens P23274Peptidyl-prolyl cis-trans isomerase B precursor 6 7 MTD73 DrosophilaAAA17887 Spatzle (spz) protein 6 melanogaster 8 MTD77 Homo sapiensNP_003231 Kaposi fibroblast growth factor (K-FGF) 3 9 MTD84 Phytophthoracactorum AAK63068 Phytotoxic protein PcF precusor 7 10 MTD85Streptomyces coelicolor NP_629842 Peptide transport system peptidebinding protein 5 11 MTD86 Streptomyces coelicolor NP_629842 Peptidetransport system secreted peptide 7 binding protein 12 MTD103 Homosapiens TMBV19 domain family member B 4 13 MTD132 Streptomycescoelicolor NP_62377 P60-family secreted protein 7 14 MTD151 Streptomycescoelicolor NP_630126 Secreted chitinase 8 15 MTD173 Streptomycescoelicolor NP_624384 Secreted protein 7 16 MTD174 Streptomycescoelicolor NP_733505 Large, multifunctional secreted protein 8 17 MTD181Neisseria meningitidis CAB84257.1 Putative secreted protein 7 Z2491

Table 2 summarizes reference information

TABLE 2 References # Title Journal Year Vol Issue Page 1 Inhibition ofNuclear Translocation of Transcription Factor JOURNAL OF 1995 270 2414255 NF-kB by a Synthetic peptide Containing a Cell Membrane-BIOLOGICAL permeable Motif and Nuclear Localization Sequence CHEMISTRY 2Epigenetic Regulation of Gene Structure and Function with NATURE 2001 1910 929 a Cell-Permeable Cre Recombinase BIOTECHNOLOGY 3 Cell-PermeableNM23 Blocks the Maintenance and CANCER 2011 71 23 7216 Progression ofEstablished Pulmonary Metastasis RESEARCH 4 Antitumor Activity OfCell-Permeable p18INK4c With MOLECULAR 2012 20 8 1540 Enhanced Membraneand Tissue Penetration THERAPY 5 Antitumor Activity of Cell-PermeableRUNX3 Protein in CLINICAL 2012 19 3 680 Gastric Cancer Cells CANCERRESEARCH 6 The Effect of Intracellular Protein Delivery on the Anti-BIOMATERIALS 2013 34 26 6261 Tumor Activity of Recombinant HumanEndostatin 7 Partial Somatic to Stem Cell Transformations Induced BySCIENTIFIC 2014 4 10 4361 Cell-Permeable Reprogramming Factors REPORTS 8Cell-Permeable Parkin Proteins Suppress Parkinson PLOS ONE 2014 9 7 17Disease-Associated Phenotypes in Cultured Cells and Animals

Table 3 shows characteristics of published hydrophobic Cell-PenetratingPeptides (A) which were analyzed.

TABLE 3 Rigidity/ Structural SEQ Flexibility Feature ID MolecularBending (Instablility (Aliphatic Hydropathy Residue A/a CompositionSecondary NOS Peptide Sequence Length Weight pl Potential Index: II)Index: AI) (GRAVY) Structure A V L I P G Structure Cargo Ref. 865 MTMAAVALLPAVLLALLAP 16 1,515.9 5.6 Bending 45.5 220.0 2.4 Aliphatic 6 2 6 02 0 Helix p50 1 Ring 866 MTS AAVLLPVLLAAP 12 1,147.4 5.6 Bending 57.3211.7 2.3 Aliphatic 4 2 4 0 2 0 No-Helix CRE 2 Ring 867 MTD10LGGAVVAAPVAAAVAP 16 1,333.5 5.5 Bending 47.9 140.6 1.8 Aliphatic 7 4 1 02 2 Helix Parkin 8 Ring 868 MTD13 LAAAALAVLPL 11 1,022.3 5.5 Bending26.6 213.6 2.4 Aliphatic 5 1 4 0 1 0 No-Helix RUNX3 5 Ring 869 MTD47AAAVPVLVAA 10 881.0 5.6 Bending 47.5 176.0 2.4 Aliphatic 5 3 1 0 1 0No-Helix CMYC 7 Ring 870 MTD56 VLLAAALIA 9 854.1 5.5 No- 8.9 250.0 3.0Aliphatic 4 1 3 1 0 0 Helix ES 6 Bending Ring 871 MTD73 PVLLLLA 7 737.96.0 No- 36.1 278.6 2.8 Aliphatic 1 1 4 0 1 0 Helix ES 6 Bending Ring 872MTD77 AVALLILAV 9 882.0 5.6 No- 30.3 271.1 3.3 Aliphatic 3 2 3 1 0 0Helix NM23 3 Bending Ring 873 MTD84 AVALVAVVAVA 11 982.2 5.6 No- 9.1212.7 3.1 Aliphatic 5 5 1 0 0 0 Helix OCT4 7 Bending Ring 874 MTD85LLAAAAALLLA 11 1,010.2 5.5 No- 9.1 231.8 2.7 Aliphatic 6 0 5 0 0 0No-Helix RUNX3 5 Bending Ring 875 MTD86 LLAAAAALLLA 11 1,010.2 5.5 No-9.1 231.8 2.7 Aliphatic 6 0 5 0 0 0 No-Helix SOX2 7 Bending Ring 876 MTD103 LALPVLLLA 9 922.2 5.5 Bending 51.7 271.1 2.8 Aliphatic 2 1 5 0 10 Helix p18 4 Ring 877  MTD132 AVVVPAIVLAAP 12 1,119.4 5.6 Bending 50.3195.0 2.4 Aliphatic 4 4 1 1 2 0 No-Helix LIN28 7 Ring 878  MTD151AAAPVAAVP 9 1,031.4 5.5 Bending 73.1 120.0 1.6 Aliphatic 5 2 0 0 2 0No-Helix Parkin 8 Ring 879  MTD173 AVIPILAVP 9 892.1 5.6 Bending 48.5216.7 2.4 Aliphatic 2 2 1 2 2 0 Helix KLF4 7 Ring 880  MTD174LILLLPAVALP 11 1,011.8 5.5 Bending 79.1 257.3 2.6 Aliphatic 2 1 5 1 2 0Helix Parkin 8 Ring 881  MTD181 AVLLLPAAA 9 838.0 5.6 Bending 51.7 206.72.4 Aliphatic 4 1 3 0 1 0 No-Helix SOX2 7 Ring AVE 10.8 ± 2.4 1,011 ±189.6 5.6 ± 0.1 Proline 40.1 ± 21.9 217.9 ± 43.6 2.5 ± 0.4 Presence

Two peptide/protein analysis programs were used (ExPasy: SoSui:http://harrier.nagahama-i-bio.ac.jp/sosui/sosui_submit.html) todetermine various indexes and structural features of the peptidesequences and to design new sequence. Followings are important factorsanalyzed.

1-2. Characteristics of Analyzed Peptides: Length, Molecular Weight andPl Value

Average length, molecular weight and pl value of the peptides analyzedwere 10.8±2.4, 1,011±189.6 and 5.6±0.1, respectively (Table 4)

Table 4 summarizes Critical Factors (CFs) of published hydrophobicCell-Penetrating Peptides (A) which were analyzed.

TABLE 4 Length: 10.8 ± 2.4 Molecular Weight: 1,011 ± 189.6 pl: 5.6 ± 0.1Bending Potential (BP): Proline presences in the middle and/or the endof peptides, or No Proline. Instability Index (II): 40.1 ± 21.9 ResidueStructure & Aliphatic Index (AI): 217.9 ± 43.6 Hydropathy (GRAVY): 2.5 ±0.4 Aliphatic Ring: Non polar hydrophobic & aliphatic amino acid (A, V,L, I). Secondary Structure: α-Helix is favored but not required.

1-3. Characteristics of Analyzed Peptides: Bending Potential—ProlinePosition (PP)

Bending potential (bending or no-bending) was determined based on thefact whether proline (P) exists and/or where the amino acid(s) providingbending potential to the peptide in recombinant protein is/are located.Proline differs from the other common amino acids in that its side chainis bonded to the backbone nitrogen atom as well as the alpha-carbonatom. The resulting cyclic structure markedly influences proteinarchitecture which is often found in the bends of folded peptide/proteinchain.

Eleven out of 17 were determined as ‘Bending’ peptide which means thatproline is present in the middle of sequence for peptide bending and/orlocated at the end of the peptide for protein bending. As indicatedabove, peptide sequences could penetrate the plasma membrane in a “bent”configuration. Therefore, bending or no-bending potential is consideredas one of the critical factors for the improvement of currenthydrophobic CPPs.

1-4. Characteristics of Analyzed Peptides:Rigidity/Flexibility—Instability Index (II)

Since one of the crucial structural features of any peptide is based onthe fact whether the motif is rigid or flexible, which is an intactphysicochemical characteristic of the peptide sequence, instabilityindex (II) of the sequence was determined. The index value representingrigidity/flexibility of the peptide was extremely varied (8.9-79.1), butaverage value was 40.1±21.9 which suggested that the peptide should besomehow flexible, but not too much rigid or flexible (Table 3).

1-5. Characteristics of Analyzed Peptides: StructuralFeatures—Structural Feature (Aliphatic Index: AI) and hydropathy (GrandAverage of Hydropathy: GRAVY)

Alanine (V), valine (V), leucine (L) and isoleucine (I) containaliphatic side chain and are hydrophobic—that is, they have an aversionto water and like to cluster. These amino acids having hydrophobicityand aliphatic residue enable them to pack together to form compactstructure with few holes. Analyzed peptide sequence showed that allcomposing amino acids were hydrophobic (A, V, L and I) except glycine(G) in only one out of 17 (MTD10—Table 3) and aliphatic (A, V, L, I, andP). Their hydropathic index (Grand Average of Hydropathy: GRAVY) andaliphatic index (AI) were 2.5±0.4 and 217.9±43.6, respectively. Theiramino acid composition is also indicated in the Table 3.

1-6. Characteristics of Analyzed Peptides: Secondary Structure(Helicity)

As explained above, the CPP sequences may be supposed to penetrate theplasma membrane directly after inserting into the membranes in a “bent”configuration with hydrophobic sequences having α-helical conformation.In addition, our analysis strongly indicated that bending potential wascrucial for membrane penetration. Therefore, structural analysis of thepeptides was conducted to determine whether the sequences were to formhelix or not. Nine peptides were helix and eight were not (Table 3). Itseems to suggest that helix structure may not be required.

1-7. Determination of Critical Factors (CFs)

In the 11 characteristics analyzed, the following 6 are selected namely“Critical Factors” for the development of new hydrophobic CPPs—advancedMTDs: amino acid length, bending potential (proline presence andlocation), rigidity/flexibility (instability index: II), structuralfeature (aliphatic index: AI), hydropathy (GRAVY) and amino acidcomposition/residue structure (hydrophobic and aliphatic A/a) (Table 3and Table 4).

2. Analysis of Selected Hydrophobic CPPs to Optimize ‘Critical Factors’

Since the analyzed data of the 17 different hydrophobic CPPs (analysisA, Table 3 and 4) previously developed during the past 2 decades showedhigh variation and were hard to make common- or consensus-features,analysis B (Table 5 and 6) and C (Table 7 and 8) were also conducted tooptimize the critical factors for better design of improved CPPs-aMTDs.Therefore, 17 hydrophobic CPPs have been grouped into two groups andanalyzed the groups for their characteristics in relation to the cellpermeable property. The critical factors have been optimized bycomparing and contrasting the analytical data of the groups anddetermining the common homologous features that may be critical for thecell permeable property.

2-1. Selective Analysis (B) of Peptides Used to Biologically ActiveCargo Protein for In Vivo

In analysis B, eight CPPs were used with each biologically active cargoin vivo. Length was 11±3.2, but 3 out of 8 CPPs possessed little bendingpotential. Rigidity/Flexibility (instability index: II) was 41±15, butremoving one [MTD85: rigid, with minimal II (9.1)] of the peptidesincreased the overall instability index to 45.6±9.3. This suggested thathigher flexibility (40 or higher II) is potentially be better. All othercharacteristics of the 8 CPPs were similar to the analysis A, includingstructural feature and hydropathy (Table 5 and 6)

Table 5 shows characteristics of published hydrophobic Cell-PenetratingPeptides (B): selected CPPs that were used to each cargo in vivo.

TABLE 5 Rigidity/ Structural SEQ Flexibility Feature ID MolecularBending (Instablility (Aliphatic NOS Peptide Sequence Length Weight plPotential Index: II) Index: AI) 865 MTM AAVALLPAVLLALLAP 16 1,515.9 5.6Bending 45.5 220.0 866 MTS AAVLLPVLLAAP 12 1,147.4 5.6 Bending 57.3211.7 867 MTD10 LGGAVVAAPVAAAVAP 16 1,333.5 5.5 Bending 47.9 140.6 871MTD73 PVLLLLA 7 737.9 6.0 No- 36.1 278.6 Bending 872 MTD77 AVALLILAV 9882.1 5.6 No- 30.3 271.1 Bending 874 MTD85 LLAAAAALLLA 11 1,010.2 5.5No- 9.1* 231.8 Bending 876  MTD103 LALPVLLLA 9 922.2 5.5 Bending 51.7271.1 877  MTD132 AVVVPAIVLAAP 12 1,119.4 5.6 Bending 50.3 195.0 AVE 11± 3.2 1,083 ± 252 5.6 ± 0.1 Proline 41 ± 15 227 ± 47 Presence SEQ IDHydropathy Residue A/a Composition Secondary NOS (GRAVY) Structure A V LI P G Structure Cargo Ref. 865 2.4 Aliphatic 6 2 6 0 2 0 Helix p50 1Ring 866 2.3 — 4 2 4 0 2 0 No-Helix CRE 2 867 1.8 — 7 4 1 0 2 2 HelixParkin 8 871 2.8 — 1 1 4 0 1 0 Helix ES 6 872 3.3 — 3 2 3 1 0 0 HelixNM23 3 874 2.7 — 6 0 5 0 0 0 No-Helix RUNX3 5 876 2.8 — 2 1 5 0 1 0Helix p18 4 877 2.4 — 4 4 1 1 2 0 No-Helix LIN28 7 2.5 ± 0.4 *Removingthe MTD85 increases II to 45.6 ± 9.3.

Table 6 shows summarized Critical Factors of published hydrophobicCell-Penetrating Peptides (B).

TABLE 6 Length: 11 ± 3.2 Molecular Weight 1,083 ± 252 pl: 5.6 ± 0.1Bending Potential (BP): Proline presences in the middle and/or the endof peptides, or No Proline. Instability Index (II): 41.0 ± 15 (′Removingthe MTD85 increases II to 45.6 ± 9.3) Residue Structure & AliphaticIndex (AI): 227 ± 47 Hydropathy (GRAVY): 2.5 ± 0.4 Aliphatic Ring:Nonpolar hydrophobic & aliphatic amino acid (A, V, L, I). SecondaryStructure: α-Helix is favored but not required.

2-2. Selective Analysis (C) of Peptides that Provided Bending Potentialand Higher Flexibility

To optimize the ‘Common Range and/or Consensus Feature of CriticalFactor’ for the practical design of aMTDs and the random peptides (rPsor rPeptides), which were to prove that the ‘Critical Factors’determined in the analysis A, B and C were correct to improve thecurrent problems of hydrophobic CPPs—protein aggregation, lowsolubility/yield, and poor cell-/tissue-permeability of the recombinantproteins fused to the MTS/MTM or MTD, and non-common sequence andnon-homologous structure of the peptides, empirically selected peptideswere analyzed for their structural features and physicochemical factorindexes.

Hydrophobic CPPs which did not have a bending potential, rigid or toomuch flexible sequences (too much low or too much high InstabilityIndex), or too low or too high hydrophobic CPPs were unselected, butsecondary structure was not considered because helix structure ofsequence was not required.

In analysis C, eight selected CPP sequences that could provide a bendingpotential and higher flexibility were finally analyzed (Table 7 and 8).Common amino acid length is 12 (11.6±3.0). Proline is presence in themiddle of and/or the end of sequence. Rigidity/Flexibility (II) is45.5-57.3 (Avg: 50.1±3.6). AI and GRAVY representing structural featureand hydrophobicity of the peptide are 204.7±37.5 and 2.4±0.3,respectively. All peptides are consisted with hydrophobic and aliphaticamino acids (A, V, L, I, and P). Therefore, analysis C was chosen as astandard for the new design of new hydrophobic CPPs—aMTDs.

Table 7 shows characteristics of published hydrophobic Cell-PenetratingPeptides (C): selected CPPs that provided bending potential and higherflexibility.

TABLE 7 Rigidity/ Structural SEQ Flexibility Feature ID MolecularBending (Instablility (Aliphatic NOS Peptide Sequence Length Weight plPotential Index: II) Index: AI) 865 MTM AAVALLPAVLLALLAP 16 1,515.9 5.6Bending 45.5 220.0 866 MTS AAVLLPVLLAAP 12 1,147.4 5.6 Bending 57.3211.7 867 MTD10  LGGAVVAAPVAAAVAP 16 1,333.5 5.5 Bending 47.9 140.6 869MTD47  AAAVPVLVAA 10 881.0 5.6 Bending 47.5 176.0 876 MTD103 LALPVLLLA 9922.2 5.5 Bending 51.7 271.1 877 MTD132 AVVVPAIVLAAP 12 1,119.4 5.6Bending 50.3 195.0 879 MTD173 AVIPILAVP 9 892.1 5.6 Bending 48.5 216.7881 MTD181 AVLLLPAAA 9 838.0 5.6 Bending 51.7 206.7 AVE 11.6 ± 3.0 1,081± 244.6 5.6 ± 0.1 Proline 50.1 ± 3.6 204.7 ± 37.5 Presence SEQ IDHydropathy Residue A/a Composition Secondary NOS (GRAVY) Structure A V LI P G Structure Cargo Ref. 865 2.4 Aliphatic 6 2 6 0 2 0 Helix p50 1Ring 866 2.3 Aliphatic 4 2 4 0 2 0 No-Helix CRE 2 Ring 867 1.8 Aliphatic7 4 1 0 2 2 Helix PARKIN 8 Ring 869 2.4 Aliphatic 5 3 1 0 1 0 No-HelixCMYC 7 Ring 876 2.8 Aliphatic 2 1 5 0 1 0 Helix p18 4 Ring INK4C 877 2.4Aliphatic 4 4 1 1 2 0 No-Helix LIN28 7 Ring 879 2.4 Aliphatic 2 2 1 2 20 Helix KLF4 7 Ring 881 2.4 Aliphatic 4 1 3 0 1 0 No-Helix SOX2 7 Ring2.4 ± 0.3

Table 8 shows summarized Critical Factors of published hydrophobicCell-Penetrating Peptides (C)

TABLE 8 Length: 11.6 ± 3.0 Molecular Weight: 1,081.2 ± 224.6 pl: 5.6 ±0.1 Bending Potential (BP): Proline presences in the middle and/or theend of peptides. Instability Index (II): 50.1 ± 3.6 Residue Structure &Aliphatic Index (AI): 204.7 ± 37.5 Hydropathy (GRAVY): 2.4 ± 0.3Aliphatic Ring: Non-polar hydrophobic & aliphatic amino acid (A, V, L,I). Secondary Structure: α-Helix is favored but not required.3. New Design of Improved Hydrophobic CPPs—aMTDs Based on the OptimizedCritical Factors

3-1. Determination of Common Sequence and/or Common Homologous Structure

As mentioned above, H-regions of signal sequence (HRSS)-derived CPPs(MTS/MTM and MTD) do not have a common sequence, sequence motif, and/orcommon-structural homologous feature. In this invention, the aim is todevelop improved hydrophobic CPPs formatted in the common sequence- andstructural-motif which satisfy newly determined ‘Critical Factors’ tohave ‘Common Function,’ namely, to facilitate protein translocationacross the membrane with similar mechanism to the analyzed referenceCPPs. Based on the analysis A, B and C, the common homologous featureshave been analyzed to determine the critical factors that influence thecell-permeability. The range value of each critical factor has beendetermined to include the analyzed index of each critical factor fromanalysis A, B and C to design novel aMTDs (Table 9). These features havebeen confirmed experimentally with newly designed aMTDs in theircell-permeability.

Table 9 shows comparison the range/feature of each Critical Factorbetween the value of analyzed CPPs and the value determined for newdesign of novel aMTDs sequences

TABLE 9 Summarized Critical Factors of aMTD Selected CPPs Newly DesignedCPPs Critical Factor Range Range Bending Potential Proline presences inProline presences in the (Proline Position: PP) the middle and/or atmiddle (5′, 6′, 7′ or 8′) the end of peptides and at the end of peptidesRigidity/Flexibility 45.5-57.3 (50.1 ± 3.6) 40-60 (Instability Index:II) Structural Feature 140.6-220.0 180-220 (Aliphatic Index: AI) (204.7± 37.5) Hydropathy  1.8-2.8 (2.4 ± 0.3) 2.1-2.6 (Grand Average ofHydropathy GRAVY) Length 11.6 ± 3.0  9-13 (Number of Amino Acid) Aminoacid Composition A, V, I, L, P A, V, I, L, P

In Table 9, universal common features and sequence/structural motif areprovided. Length is 9-13 amino acids, and bending potential is providedwith the presence of proline in the middle of sequence (at 5′, 6′, 7′ or8′ amino acid) for peptide bending and at the end of peptide forrecombinant protein bending and Rigidity/Flexibility of aMTDs is II>40are described in Table 9.

3-2. Critical Factors for Development of Advanced MTDs

Recombinant cell-permeable proteins fused to the hydrophobic CPPs todeliver therapeutically active cargo molecules including proteins intolive cells had previously been reported, but the fusion proteinsexpressed in bacteria system were hard to be purified as a soluble formdue to their low solubility and yield. To address the crucial weaknessfor further clinical development of the cell-permeable proteins asprotein-based biotherapeutics, greatly improved form of the hydrophobicCPP, named as advanced MTD (aMTD) has newly been developed throughcritical factors-based peptide analysis. The critical factors used forthe current invention of the aMTDs are herein (Table 9).

1. Amino Acid Length: 9-13

2. Bending Potential (Proline Position: PP)

: Proline presences in the middle (from 5′ to 8′ amino acid) and at theend of sequence

3. Rigidity/Flexibility (Instability Index: II): 40-60

4. Structural Feature (Aliphatic Index: AI): 180-220

5. Hydropathy (GRAVY): 2.1-2.6

6. Amino Acid Composition: Hydrophobic and Aliphatic amino acids—A, V,L, I and P

3-3. Design of Potentially Best aMTDs that all Critical Factors areConsidered and Satisfied

After careful consideration of six critical factors derived fromanalysis of unique features of hydrophobic CPPs, advanced macromoleculetransduction domains (aMTDs) have been designed and developed based onthe common 12 amino acid platform which satisfies the critical factorsincluding amino acid length (9-13) determined from the analysis.

Unlike previously published hydrophobic CPPs that require numerousexperiments to determine their cell-permeability, newly developed aMTDsequences could be designed by performing just few steps as followsusing above mentioned platform to follow the determined rangevalue/feature of each critical factor.

First, prepare the 12 amino acid sequence platform for aMTD. Second,place proline (P) in the end (12′) of sequence and determine where toplace proline in one of four U(s) in 5′, 6′, 7′, and 8. Third, alanine(A), valine (V), leucine (L) or isoleucine (I) is placed in either X(s)and/or U(s), where proline is not placed. Lastly, determine whether theamino acid sequences designed based on the platform, satisfy the valueor feature of six critical factors to assure the cell permeable propertyof aMTD sequences. Through these processes, numerous novel aMTDsequences have been constructed. The expression vectors for preparingnon-functional cargo recombinant proteins fused to each aMTD, expressionvectors have been constructed and forcedly expressed in bacterial cells.These aMTD-fused recombinant proteins have been purified in soluble formand determined their cell-permeability quantitatively. aMTD sequenceshave been newly designed, numbered from 1 to 240, as shown in Table10-15. In Table 10-15, sequence ID Number is a sequence listings forreference, and aMTD numbers refer to amino acid listing numbers thatactually have been used at the experiments. For further experiments,aMTD numbers have been used. In addition, polynucleotide sequences shownin the sequence lists have been numbered from SEQ ID NO: 241 to SEQ IDNO: 480.

Tables 10 to 15 shows 240 new hydrophobic aMTD sequences that weredeveloped to satisfy all critical factors.

TABLE 10 Sequence Rigidity/ Structural Hydro- ID Flexibility Featurepathy Residue Number aMTD Sequences Length (II) (AI) (GRAVY) Structure 1  1 AAALAPVVLALP 12 57.3 187.5 2.1 Aliphatic  2  2 AAAVPLLAVVVP 1241.3 195.0 2.4 Aliphatic  3  3 AALLVPAAVLAP 12 57.3 187.5 2.1 Aliphatic 4  4 ALALLPVAALAP 12 57.3 195.8 2.1 Aliphatic  5  5 AAALLPVALVAP 1257.3 187.5 2.1 Aliphatic  6 11 VVALAPALAALP 12 57.3 187.5 2.1 Aliphatic 7 12 LLAAVPAVLLAP 12 57.3 211.7 2.3 Aliphatic  8 13 AAALVPVVALLP 1257.3 203.3 2.3 Aliphatic  9 21 AVALLPALLAVP 12 57.3 211.7 2.3 Aliphatic10 22 AVVLVPVLAAAP 12 57.3 195.0 2.4 Aliphatic 11 23 VVLVLPAAAAVP 1257.3 195.0 2.4 Aliphatic 12 24 IALAAPALIVAP 12 50.2 195.8 2.2 Aliphatic13 25 IVAVAPALVALP 12 50.2 203.3 2.4 Aliphatic 14 42 VAALPVVAVVAP 1257.3 186.7 2.4 Aliphatic 15 43 LLAAPLVVAAVP 12 41.3 187.5 2.1 Aliphatic16 44 ALAVPVALLVAP 12 57.3 203.3 2.3 Aliphatic 17 61 VAALPVLLAALP 1257.3 211.7 2.3 Aliphatic 18 62 VALLAPVALAVP 12 57.3 203.3 2.3 Aliphatic19 63 AALLVPALVAVP 12 57.3 203.3 2.3 Aliphatic

TABLE 11 Sequence Rigidity/ Structural Hydro- ID Flexibility Featurepathy Residue Number aMTD Sequences Length (II) (AI) (GRAVY) Structure20 64 AIVALPVAVLAP 12 50.2 203.3 2.4 Aliphatic 21 65 IAIVAPVVALAP 1250.2 203.3 2.4 Aliphatic 22 81 AALLPALAALLP 12 57.3 204.2 2.1 Aliphatic23 82 AVVLAPVAAVLP 12 57.3 195.0 2.4 Aliphatic 24 83 LAVAAPLALALP 1241.3 195.8 2.1 Aliphatic 25 84 AAVAAPLLLALP 12 41.3 195.8 2.1 Aliphatic26 85 LLVLPAAALAAP 12 57.3 195.8 2.1 Aliphatic 27 101 LVALAPVAAVLP 1257.3 203.3 2.3 Aliphatic 28 102 LALAPAALALLP 12 57.3 204.2 2.1 Aliphatic29 103 ALIAAPILALAP 12 57.3 204.2 2.2 Aliphatic 30 104 AVVAAPLVLALP 1241.3 203.3 2.3 Aliphatic 31 105 LLALAPAALLAP 12 57.3 204.1 2.1 Aliphatic32 121 AIVALPALALAP 12 50.2 195.8 2.2 Aliphatic 33 123 AAIIVPAALLAP 1250.2 195.8 2.2 Aliphatic 34 124 IAVALPALIAAP 12 50.3 195.8 2.2 Aliphatic35 141 AVIVLPALAVAP 12 50.2 203.3 2.4 Aliphatic 36 143 AVLAVPAVLVAP 1257.3 195.0 2.4 Aliphatic 37 144 VLAIVPAVALAP 12 50.2 203.3 2.4 Aliphatic38 145 LLAVVPAVALAP 12 57.3 203.3 2.3 Aliphatic 39 161 AVIALPALIAAP 1257.3 195.8 2.2 Aliphatic 40 162 AVVALPAALIVP 12 50.2 203.3 2.4 Aliphatic41 163 LALVLPAALAAP 12 57.3 195.8 2.1 Aliphatic 42 164 LAAVLPALLAAP 1257.3 195.8 2.1 Aliphatic 43 165 ALAVPVALAIVP 12 50.2 203.3 2.4 Aliphatic44 182 ALIAPVVALVAP 12 57.3 203.3 2.4 Aliphatic 45 183 LLAAPVVIALAP 1257.3 211.6 2.4 Aliphatic 46 184 LAAIVPAIIAVP 12 50.2 211.6 2.4 Aliphatic47 185 AALVLPLIIAAP 12 41.3 220.0 2.4 Aliphatic 48 201 LALAVPALAALP 1257.3 195.8 2.1 Aliphatic 49 204 LIAALPAVAALP 12 57.3 195.8 2.2 Aliphatic50 205 ALALVPAIAALP 12 57.3 195.8 2.2 Aliphatic 51 221 AAILAPIVALAP 1250.2 195.8 2.2 Aliphatic 52 222 ALLIAPAAVIAP 12 57.3 195.8 2.2 Aliphatic53 223 AILAVPIAVVAP 12 57.3 203.3 2.4 Aliphatic 54 224 ILAAVPIALAAP 1257.3 195.8 2.2 Aliphatic 55 225 VAALLPAAAVLP 12 57.3 187.5 2.1 Aliphatic56 241 AAAVVPVLLVAP 12 57.3 195.0 2.4 Aliphatic 57 242 AALLVPALVAAP 1257.3 187.5 2.1 Aliphatic 58 243 AAVLLPVALAAP 12 57.3 187.5 2.1 Aliphatic59 245 AAALAPVLALVP 12 57.3 187.5 2.1 Aliphatic 60 261 LVLVPLLAAAAP 1241.3 211.6 2.3 Aliphatic 61 262 ALIAVPAIIVAP 12 50.2 211.6 2.4 Aliphatic62 263 ALAVIPAAAILP 12 54.9 195.8 2.2 Aliphatic 63 264 LAAAPVVIVIAP 1250.2 203.3 2.4 Aliphatic 64 265 VLAIAPLLAAVP 12 41.3 211.6 2.3 Aliphatic65 281 ALIVLPAAVAVP 12 50.2 203.3 2.4 Aliphatic 66 282 VLAVAPALIVAP 1250.2 203.3 2.4 Aliphatic 67 283 AALLAPALIVAP 12 50.2 195.8 2.2 Aliphatic68 284 ALIAPAVALIVP 12 50.2 211.7 2.4 Aliphatic 69 285 AIVLLPAAVVAP 1250.2 203.3 2.4 Aliphatic

TABLE 12 Sequence Rigidity/ Structural Hydro- ID Flexibility Featurepathy Residue Number aMTD Sequences Length (II) (AI) (GRAVY) Structure 70 301 VIAAPVLAVLAP 12 57.3 203.3 2.4 Aliphatic  71 302 LALAPALALLAP 1257.3 204.2 2.1 Aliphatic  72 304 AIILAPIAAIAP 12 57.3 204.2 2.3Aliphatic  73 305 IALAAPILLAAP 12 57.3 204.2 2.2 Aliphatic  74 321IVAVALPALAVP 12 50.2 203.3 2.3 Aliphatic  75 322 VVAIVLPALAAP 12 50.2203.3 2.3 Aliphatic  76 323 IVAVALPVALAP 12 50.2 203.3 2.3 Aliphatic  77324 IVAVALPAALVP 12 50.2 203.3 2.3 Aliphatic  78 325 IVAVALPAVALP 1250.2 203.3 2.3 Aliphatic  79 341 IVAVALPAVLAP 12 50.2 203.3 2.3Aliphatic  80 342 VIVALAPAVLAP 12 50.2 203.3 2.3 Aliphatic  81 343IVAVALPALVAP 12 50.2 203.3 2.3 Aliphatic  82 345 ALLIVAPVAVAP 12 50.2203.3 2.3 Aliphatic  83 361 AVVIVAPAVIAP 12 50.2 195.0 2.4 Aliphatic  84363 AVLAVAPALIVP 12 50.2 203.3 2.3 Aliphatic  85 364 LVAAVAPALIVP 1250.2 203.3 2.3 Aliphatic  86 365 AVIVVAPALLAP 12 50.2 203.3 2.3Aliphatic  87 381 VVAIVLPAVAAP 12 50.2 195.0 2.4 Aliphatic  88 382AAALVIPAILAP 12 54.9 195.8 2.2 Aliphatic  89 383 VIVALAPALLAP 12 50.2211.6 2.3 Aliphatic  90 384 VIVAIAPALLAP 12 50.2 211.6 2.4 Aliphatic  91385 IVAIAVPALVAP 12 50.2 203.3 2.4 Aliphatic  92 401 AALAVIPAAILP 1254.9 195.8 2.2 Aliphatic  93 402 ALAAVIPAAILP 12 54.9 195.8 2.2Aliphatic  94 403 AAALVIPAAILP 12 54.9 195.8 2.2 Aliphatic  95 404LAAAVIPAAILP 12 54.9 195.8 2.2 Aliphatic  96 405 LAAAVIPVAILP 12 54.9211.7 2.4 Aliphatic  97 421 AAILAAPLIAVP 12 57.3 195.8 2.2 Aliphatic  98422 VVAILAPLLAAP 12 57.3 211.7 2.4 Aliphatic  99 424 AVVVAAPVLALP 1257.3 195.0 2.4 Aliphatic 100 425 AVVAIAPVLALP 12 57.3 203.3 2.4Aliphatic 101 442 ALAALVPAVLVP 12 57.3 203.3 2.3 Aliphatic 102 443ALAALVPVALVP 12 57.3 203.3 2.3 Aliphatic 103 444 LAAALVPVALVP 12 57.3203.3 2.3 Aliphatic 104 445 ALAALVPALVVP 12 57.3 203.3 2.3 Aliphatic 105461 IAAVIVPAVALP 12 50.2 203.3 2.4 Aliphatic 106 462 IAAVLVPAVALP 1257.3 203.3 2.4 Aliphatic 107 463 AVAILVPLLAAP 12 57.3 211.7 2.4Aliphatic 108 464 AVVILVPLAAAP 12 57.3 203.3 2.4 Aliphatic 109 465IAAVIVPVAALP 12 50.2 203.3 2.4 Aliphatic 110 481 AIAIAIVPVALP 12 50.2211.6 2.4 Aliphatic 111 482 ILAVAAIPVAVP 12 54.9 203.3 2.4 Aliphatic 112483 ILAAAIIPAALP 12 54.9 204.1 2.2 Aliphatic 113 484 LAVVLAAPAIVP 1250.2 203.3 2.4 Aliphatic 114 485 AILAAIVPLAVP 12 50.2 211.6 2.4Aliphatic 115 501 VIVALAVPALAP 12 50.2 203.3 2.4 Aliphatic 116 502AIVALAVPVLAP 12 50.2 203.3 2.4 Aliphatic 117 503 AAIIIVLPAALP 12 50.2220.0 2.4 Aliphatic 118 504 LIVALAVPALAP 12 50.2 211.7 2.4 Aliphatic 119505 AIIIVIAPAAAP 12 50.2 195.8 2.3 Aliphatic

TABLE 13 Sequence Rigidity/ Structural Hydro- ID Flexibility Featurepathy Residue Number aMTD Sequences Length (II) (AI) (GRAVY) Structure120 521 LAALIVVPAVAP 12 50.2 203.3 2.4 Aliphatic 121 522 ALLVIAVPAVAP 1257.3 203.3 2.4 Aliphatic 122 524 AVALIVVPALAP 12 50.2 203.3 2.4Aliphatic 123 525 ALAIVVAPVAVP 12 50.2 195.0 2.4 Aliphatic 124 541LLALIIAPAAAP 12 57.3 204.1 2.1 Aliphatic 125 542 ALALIIVPAVAP 12 50.2211.6 2.4 Aliphatic 126 543 LLAAL1APAALP 12 57.3 204.1 2.1 Aliphatic 127544 IVALIVAPAAVP 12 43.1 203.3 2.4 Aliphatic 128 545 VVLVLAAPAAVP 1257.3 195.0 2.3 Aliphatic 129 561 AAVAIVLPAVVP 12 50.2 195.0 2.4Aliphatic 130 562 ALIAAIVPALVP 12 50.2 211.7 2.4 Aliphatic 131 563ALAVIVVPALAP 12 50.2 203.3 2.4 Aliphatic 132 564 VAIALIVPALAP 12 50.2211.7 2.4 Aliphatic 133 565 VAIVLVAPAVAP 12 50.2 195.0 2.4 Aliphatic 134582 VAVALIVPALAP 12 50.2 203.3 2.4 Aliphatic 135 583 AVILALAPIVAP 1250.2 211.6 2.4 Aliphatic 136 585 ALIVAIAPALVP 12 50.2 211.6 2.4Aliphatic 137 601 AAILIAVPIAAP 12 57.3 195.8 2.3 Aliphatic 138 602VIVALAAPVLAP 12 50.2 203.3 2.4 Aliphatic 139 603 VLVALAAPVIAP 12 57.3203.3 2.4 Aliphatic 140 604 VALIAVAPAVVP 12 57.3 195.0 2.4 AllPhatie 141605 VIAAVLAPVAVP 12 57.3 195.0 2.4 Aliphatic 142 622 ALIVLAAPVAVP 1250.2 203.3 2.4 Aliphatic 143 623 VAAAIALPAIVP 12 50.2 187.5 2.3Aliphatic 144 625 ILAAAAAPLIVP 12 50.2 195.8 2.2 Aliphatic 145 643LALVLAAPAIVP 12 50.2 211.6 2.4 Aliphatic 146 645 ALAVVALPAIVP 12 50.2203.3 2.4 Aliphatic 147 661 AAILAPIVAALP 12 50.2 195.8 2.2 Aliphatic 148664 ILIAIAIPAAAP 12 54.9 204.1 2.3 Aliphatic 149 665 LAIVLAAPVAVP 1250.2 203.3 2.3 Aliphatic 150 666 AAIAIIAPAIVP 12 50.2 195.8 2.3Aliphatic 151 667 LAVAIVAPALVP 12 50.2 203.3 2.3 Aliphatic 152 683LAIVLAAPAVLP 12 50.2 211.7 2.4 Aliphatic 153 684 AAIVLALPAVLP 12 50.2211.7 2.4 Aliphatic 154 685 ALLYAVLPAALP 12 57.3 211.7 2.3 Aliphatic 155686 AALVAVLPVALP 12 57.3 203.3 2.3 Aliphatic 156 687 AILAVALPLLAP 1257.3 220.0 2.3 Aliphatic 157 703 IVAVALVPALAP 12 50.2 203.3 2.4Aliphatic 158 705 IVAVALLPALAP 12 50.2 211.7 2.4 Aliphatic 159 706IVAVALLPAVAP 12 50.2 203.3 2.4 Aliphatic 160 707 IVALAVLPAVAP 12 50.2203.3 2.4 Aliphatic 161 724 VAVLAVLPALAP 12 57.3 203.3 2.3 Aliphatic 162725 IAVLAVAPAVLP 12 57.3 203.3 2.3 Aliphatic 163 726 LAVAIIAPAVAP 1257.3 187.5 2.2 Aliphatic 164 727 VALAIALPAVLP 12 57.3 211.6 2.3Aliphatic 165 743 AIAIALVPVALP 12 57.3 211.6 2.4 Aliphatic 166 744AAVVIVAPVALP 12 50.2 195.0 2.4 Aliphatic 167 746 VAIIVVAPALAP 12 50.2203.3 2.4 Aliphatic 168 747 VALLAIAPALAP 12 57.3 195.8 2.2 Aliphatic 169763 VAVLIAVPALAP 12 57.3 203.3 2.3 Aliphatic

TABLE 14 Sequence Rigidity/ Structural Hydro- ID Flexibility Featurepathy Residue Number aMTD Sequences Length (II) (AI) (GRAVY) Structure170 764 AVALAVLPAVVP 12 57.3 195.0 2.3 Aliphatic 171 765 AVALAVVPAVLP 1257.3 195.0 2.3 Aliphatic 172 766 IVVIAVAPAVAP 12 50.2 195.0 2.4Aliphatic 173 767 IVVAAVVPALAP 12 50.2 195.0 2.4 Aliphatic 174 783IVALVPAVAIAP 12 50.2 203.3 2.5 Aliphatic 175 784 VAALPAVALVVP 12 57.3195.0 2.4 Aliphatic 176 786 LVAIAPLAVLAP 12 41.3 211.7 2.4 Aliphatic 177787 AVALVPVIVAAP 12 50.2 195.0 2.4 Aliphatic 178 788 AIAVAIAPVALP 1257.3 187.5 2.3 Aliphatic 179 803 AIALAVPVLALP 12 57.3 211.7 2.4Aliphatic 180 805 LVLIAAAPIALP 12 41.3 220.0 2.4 Aliphatic 181 806LVALAVPAAVLP 12 57.3 203.3 2.3 Aliphatic 182 807 AVALAVPALVLP 12 57.3203.3 2.3 Aliphatic 183 808 LVVLAAAPLAVP 12 41.3 203.3 2.3 Aliphatic 184809 LIVLAAPALAAP 12 50.2 195.8 2.2 Aliphatic 185 810 VIVLAAPALAAP 1250.2 187.5 2.2 Aliphatic 186 811 AVVLAVPALAVP 12 57.3 195.0 2.3Aliphatic 187 824 LIIVAAAPAVAP 12 50.2 187.5 2.3 Aliphatic 188 825IVAVIVAPAVAP 12 43.2 195.0 2.5 Aliphatic 189 826 LVALAAPIIAVP 12 41.3211,7 2.4 Aliphatic 190 827 IAAVLAAPALVP 12 57.3 187.5 2.2 Aliphatic 191828 IALLAAPIIAVP 12 41.3 220.0 2.4 Aliphatic 192 829 AALALVAPVIVP 1250.2 203.3 2.4 Aliphatic 193 830 IALVAAPVALVP 12 57.3 203.3 2.4Aliphatic 194 831 IIVAVAPAAIVP 12 43.2 203.3 2.5 Aliphatic 195 832AVAAIVPVIVAP 12 43.2 195.0 2.5 Aliphatic 196 843 AVLVLVAPAAAP 12 41.3219.2 2.5 Aliphatic 197 844 VVALLAPLIAAP 12 41.3 211.8 2.4 Aliphatic 198845 AAVVIAPLLAVP 12 41.3 203.3 2.4 Aliphatic 199 846 IAVAVAAPLLVP 1241.3 200.3 2.4 Aliphatic 200 847 LVAIVVLPAVAP 12 50.2 219.2 2.6Aliphatic 201 848 AVAIVVLPAVAP 12 50.2 195.0 2.4 Aliphatic 202 849AVILLAPLIAAP 12 57.3 220.0 2.4 Aliphatic 203 850 LVIALAAPVALP 12 57.3211.7 2.4 Aliphatic 204 851 VLAVVLPAVALP 12 57.3 219.2 2.5 Aliphatic 205852 VLAVAAPAVLLP 12 57.3 203.3 2.3 Aliphatic 206 863 AAVVLLPIIAAP 1241.3 211.7 2.4 Aliphatic 207 864 ALLVIAPAIAVP 12 57.3 211.7 2.4Aliphatic 208 865 AVLVIAVPAIAP 12 57.3 203.3 2.5 Aliphatic 209 867ALLVVIAPLAAP 12 41.3 211.7 2.4 Aliphatic 210 868 VLVAAILPAAIP 12 54.9211.7 2.4 Aliphatic 211 870 VLVAAVLPIAAP 12 41.3 203.3 2.4 Aliphatic 212872 VLAAAVLPLVVP 12 41.3 219.2 2.5 Aliphatic 213 875 AIAIVVPAVAVP 1250.2 195.0 2.4 Aliphatic 214 877 VAIIAVPAVVAP 12 57.3 195.0 2.4Aliphatic 215 878 IVALVAPAAVVP 12 50.2 195.0 2.4 Aliphatic 216 879AAIVLLPAVVVP 12 50.2 219.1 2.5 Aliphatic 217 881 AALIVVPAVAVP 12 50.2195.0 2.4 Aliphatic 218 882 AIALVVPAVAVP 12 57.3 195.0 2.4 Aliphatic 219883 LAIVPAAIAALP 12 50.2 195.8 2.2 Aliphatic

TABLE 15 Sequence Rigidity/ Structural Hydro- ID Flexibility Featurepathy Residue Number aMTD Sequences Length (II) (AI) (GRAVY) Structure220 885 LVAIAPAVAVLP 12 57.3 203.3 2.4 Aliphatic 221 887 VLAVAPAVAVLP 1257.3 195.0 2.4 Aliphatic 222 888 ILAVVAIPAAAP 12 54.9 187.5 2.3Aliphatic 223 889 ILVAAAPIAALP 12 57.3 195.8 2.2 Aliphatic 224 891ILAVAAIPAALP 12 54.9 195.8 2.2 Aliphatic 225 893 VIAIPAILAAAP 12 54.9195.8 2.3 Aliphatic 226 895 AIIIVVPAIAAP 12 50.2 211.7 2.5 Aliphatic 227896 AILIVVAPIAAP 12 50.2 211.7 2.5 Aliphatic 228 897 AVIVPVAIIAAP 1250.2 203.3 2.5 Aliphatic 229 899 AVVIALPAVVAP 12 57.3 195.0 2.4Aliphatic 230 900 ALVAVIAPVVAP 12 57.3 195.0 2.4 Aliphatic 231 901ALVAVLPAVAVP 12 57.3 195.0 2.4 Aliphatic 232 902 ALVAPLLAVAVP 12 41.3203.3 2.3 Aliphatic 233 904 AVLAVVAPVVAP 12 57.3 186.7 2.4 Aliphatic 234905 AVIAVAPLVVAP 12 41.3 195.0 2.4 Aliphatic 235 906 AVIALAPVVVAP 1257.3 195.0 2.4 Aliphatic 236 907 VAIALAPVVVAP 12 57.3 195.0 2.4Aliphatic 237 908 VALALAPVVVAP 12 57.3 195.0 2.3 Aliphatic 238 910VAALLPAVVVAP 12 57.3 195,0 2.3 Aliphatic 239 911 VALALPAVVVAP 12 57.3195.0 2.3 Aliphatic 240 912 VALLAPAVVVAP 12 57.3 195.0 2.3 Aliphatic52.6 ± 05.1 201.7 ± 7.8 2.3 ± 0.1

3-4. Design of the Peptides that Did not Satisfy at Least One CriticalFactor

To demonstrate that this invention of new hydrophobic CPPs-aMTDs, whichsatisfy all critical factors described above, are correct and rationallydesigned, the peptides which do not satisfy at least one critical factorhave also been designed. Total of 31 rPeptides (rPs) are designed,developed and categorized as follows: no bending peptides, either noproline in the middle as well at the end and/or no central proline;rigid peptides (II<40); too much flexible peptides; aromatic peptides(aromatic ring presences); hydrophobic, with non-aromatic peptides buthave amino acids other than A, V, L, I, P or additional prolineresidues; hydrophilic, but non-aliphatic peptides.

3-4-1. Peptides that do not Satisfy the Bending Potential

Table 16 shows the peptides that do not have any proline in the middle(at 5′, 6′, 7′ or 8′) and at the end of the sequences. In addition,Table 16 describes the peptides that do not have proline in the middleof the sequences. All these peptides are supposed to have no-bendingpotential.

TABLE 16 rPeptide Proline Rigidity/ Structural Hydro- ID (SEQ PositionFlexibility Feature pathy Group ID NO) Sequences Length (PP) (II) (AI)(GRAVY) No-Bending 931 (882) AVLIAPAILAAA 12  6 57.3 204.2  2.5 Peptides936 (883) ALLILAAAVAAP 12 12 41.3 204.2  2.4 [No Proline 152 (884)LAAAVAAVAALL 12 None  9.2 204.2  2.7 at 5, 6, 7  27 (885) LAIVAAAAALVA12 None  2.1 204.2  2.8 or 8 and/or 12] 935 (886) ALLILPAAAVAA 12  657.3 204.2  2.4 670 (887) ALLILAAAVAAL 12 None 25.2 236.6  2.8 934 (888)LILAPAAVVAAA 12 5 57.3 195.8  2.5  37 (889) TTCSQQQYCTNG 12 None 53.1  0.0 -1.1  16 (890) NNSCTTYTNGSQ 12 None 47.4   0.0 -1.4 113 (891)PVAVALLIAVPP 12 1, 11, 12 57.3 195.0  2.1

3-4-2. Peptides that do not Satisfy the Rigidity/Flexibility

To prove that rigidity/flexibility of the sequence is a crucial criticalfactor, rigid (Avg. II: 21.8±6.6) and too high flexible sequences (Avg.II: 82.3±21.0) were also designed. Rigid peptides that instability indexis much lower than that of new aMTDs (II: 41.3-57.3, Avg. II: 53.3±5.7)are shown in Table 17. Bending, but too high flexible peptides that IIis much higher than that of new aMTDs are also provided in Table 18.

TABLE 17 rPeptide Proline Rigidity/ Structural Hydro- ID (SEQ PositionFlexibility Feature pathy Group ID NO) Sequences Length (PP) (II) (AI)(GRAVY) Rigid 226 (892) ALVAAIPALAIP 12 6 20.4 195.8  2.2 Peptides  6 (893) VIAMIPAAFWVA 12 6 15.7 146.7  2.2 [II < 50] 750 (894)LAIAAIAPLAIP 12 8, 12 22.8 204.2  2.2  26 (895) AAIALAAPLAIV 12 8 18.1204.2  2.5 527 (896) LVLAAVAPIAIP 12 8, 12 22.8 211.7  2.4 466 (897)IIAAAAPLAIIP 12 7, 12 22.8 204.2  2.3 167 (898) VAIAIPAALAIP 12 6, 1220.4 195.8  2.3 246 (899) VVAVPLLVAFAA 12 5 25.2 195.0  2.7 426 (900)AAALAIPLAIIP 12 7, 12 4.37 204.2  2.2 606 (901) AAAIAAIPIIIP 12 8, 12 4.4 204.2  2.4  66 (902) AGVLGGPIMGVP 12 7, 12 35.5 121.7  1.3248 (903) VAAIVPIAALVP 12 6, 12 34.2 203.3  2.5 227 (904) LAAIVPIAAAVP12 6, 12 34.2 187.5  2.2  17 (905) GGCSAPQTTCSN 12 6 51.6   8.3 -0.5 67 (906) LDAEVPLADDVP 12 6, 12 34.2 130.0  0.3

TABLE 18 rPeptide Proline Rigidity/ Structural Hydro- ID (SEQ PositionFlexibility Feature pathy Group ID NO) Sequences Length (PP) (II) (AI)(GRAVY) Bending 692 (907) PAPLPPVVILAV 12 1, 3, 5, 105.5 186.7 1.8Peptides, 6 but Too High  69 (908) PVAVLPPAALVP 12 1, 6, 7,  89.4 162.51.6 Flexibility 12 390 (909) VPLLVPVVPVVP 12 2, 6, 9, 105.4 210.0 2.2 12350 (910) VPILVPVVPVVP 12 2, 6, 9, 121.5 210.0 2.2 12 331 (911)VPVLVPLVPVVP 12 2, 6, 9, 105.4 210.0 2.2 12   9 (912) VALVPAALILPP 125, 11, 12  89.4 203.3 2.1  68 (913) VAPVLPAAPLVP 12 3, 6, 9, 105.5 162.51.6 12 349 (914) VPVLVPVVPVVP 12 2, 6, 9, 121.5 201.6 2.2 12 937 (915)VPVLVPLPVPVV 12 2, 6, 8, 121.5 210.0 2.2 10 938 (916) VPVLLPVVVPVP 122, 6, 10, 121.5 210.0 2.2 12 329 (917) LPVLVPVVPVVP 12 2, 6, 9, 121.5210.0 2.2 12  49 (918) VVPAAPAVPVVP 12 3, 6, 9, 121.5 145.8 1.7 12772 (919) LPVAPVIPIIVP 12 2, 5, 8,  79.9 210.8 2.1 12 210 (920)ALIALPALPALP 12 6, 9, 12  89.4 195.8 1.8  28 (921) AVPLLPLVPAVP 123, 6, 9,  89.4 185.8 1.8 12 693 (922) AAPVLPVAVPIV 12 3, 6, 10  82.3185.7 2.1 169 (923) VALVAPALILAP 12 6, 12  73.4 211.7 2.4  29 (924)VLPPLPVLPVLP 12 3, 4, 6, 121.5 202.5 1.7 9, 12 190 (925) AAILAPAVIAPP 126, 11, 12  89.4 163.3 1.8

3-4-3. Peptides that do not Satisfy the Structural Features

New hydrophobic CPPs-aMTDs are consisted with only hydrophobic andaliphatic amino acids (A, V, L, I and P) with average ranges of theindexes—AI: 180-220 and GRAVY: 2.1-2.6 (Table 9). Based on thestructural indexes, the peptides which contain an aromatic residue (W, For Y) are shown in Table 19 and the peptides which are hydrophobic withnon-aromatic sequences but have amino acids residue other than A, V, L,I, P or additional proline residues are designed (Table 20). Finally,hydrophilic and/or bending peptides which are consisted withnon-aliphatic amino acids are shown in Table 21.

TABLE 19 rPeptide Proline Rigidity/ Structural Hydro- ID (SEQ PositionFlexibility Feature pathy Group ID NO) Sequences Length (PP) (II) (AI)(GRAVY) Aromatic  30 (926) AMALLPAAVAVA 12 6 51.6 163.3 2.3 Peptides 33 (927) AAAILAPAFLAV 12 7 57.3 171.7 2.4 [Aromatic 131 (928)WIIAPVWLAWIA 12 5 51.6 179.2 1.9 Ring 922 (929) WYVIFVLPLVVP 12 8, 1241.3 194.2 2.2 Presence]  71 (930) FMWMWFPFMWYP 12 7, 12 71.3   0.0 0.6921 (931) IWWFVVLPLVVP 12 8, 12 41.3 194.2 2.2

TABLE 20 rPeptide Proline Rigidity/ Structural Hydro- ID (SEQ PositionFlexibility Feature pathy Group ID NO) Sequences Length (PP) (II) (AI)(GRAVY) Hydrophobic 436 (932) VVMLVVPAVMLP 12 7, 12 57.3 194.2 2.6Peptides, 138 (933) PPAALLAILAVA 12 1, 2 57.3 195.8 2.2 but Non 77 (934) PVALVLVALVAP 12 1, 12 41.3 219.2 2.5 Aromatic 577 (935)MLMIALVPMIAV 12 8 18.9 195.0 2.7 Peptides  97 (936) ALLAAPPALLAL 12 6, 757.3 204.2 2.1 214 (937) ALIVAPALMALP 12 6, 12 60.5 187.5 2.2  59 (938)AVLAAPVVAALA 12 6 41.3 187.5 2.5  54 (939) LAVAAPPVVALL 12 6, 7 57.3203.3 2.3

TABLE 21 rPeptide Proline Rigidity/ Structural Hydro- ID (SEQ PositionFlexibility Feature pathy Group ID NO) Sequences Length (PP) (II) (AI)(GRAVY) Hydrophilic 949 (940) SGNSCQQCGNSS 12 None 41.7 0.0 -1.1Peptides,  39 (941) CYNTSPCTGCCY 12  6 52.5 0.0  0.0 but Non  19 (942)YVSCCTYTNGSQ 12 None 47.7 0.0 -1.0 Aliphatic 947 (943) CYYNQQSNNNNQ 12None 59.6 0.0 -2.4 139 (944) TGSTNSPTCTST 12  7 53.4 0.0 -0.7  18 (945)NYCCTPTTNGQS 12  6 47.9 0.0 -0.9  20 (946) NYCNTCPTYGQS 12  7 47.4 0.0-0.9 635 (947) GSTGGSQQNNQY 12 None 31.9 0.0 -1.9  40 (948) TYNTSCTPGTCY12  8 49.4 0,0 -0.6  57 (949) QNNCNTSSQGGG 12 None 52.4 0.0 -1.6159 (950) CYSGSTSQNQPP 12 11, 12 51.0 0.0 -1.3 700 (951) GTSNTCQSNQNS 12None 19.1 0.0 -1.6  38 (952) YYNQSTCGGQCY 12 None 53.8 0.0 -1.0

3-5. Summary of Newly Designed Peptides

Total of 457 sequences have been designed based on the critical factors.Designed potentially best aMTDs (hydrophobic, flexible, bending,aliphatic and 12-A/a length peptides) that do satisfy all range/featureof critical factors are 316. Designed rPeptides that do not satisfy atleast one of the critical factors are 141 that no bending peptidesequences are 26; rigid peptide (II<40) sequences are 23; too muchflexible peptides are 24; aromatic peptides (aromatic ring presences)are 27; hydrophobic, but non-aromatic peptides are 23; and hydrophilic,but non-aliphatic peptides are 18.

4. Preparation of Recombinant Report Proteins Fused to aMTDs andrPeptides

Recombinant proteins fused to aMTDs and others [rPeptides, referencehydrophobic CPP sequences (MTM and MTD)] were expressed in a bacterialsystem, purified with single-step affinity chromatography and preparedas soluble proteins in physiological condition. These recombinantproteins have been tested for the ability of their cell-permeability byutilizing flow cytometry and laser scanning confocal microscopy.

4-1. Selection of Cargo Protein for Recombinant Proteins Fused toPeptide Sequences

For clinical/non-clinical application, aMTD-fused cargo materials wouldbe biologically active molecules that could be one of the following:enzymes, transcription factors, toxic, antigenic peptides, antibodiesand antibody fragments. Furthermore, biologically active molecules couldbe one of these following macromolecules: enzymes, hormones, carriers,immunoglobulin, membrane-bound proteins, transmembrane proteins,internal proteins, external proteins, secreted proteins, virus proteins,native proteins, glycoproteins, fragmented proteins, disulfide bondedproteins, recombinant proteins, chemically modified proteins and prions.In addition, these biologically active molecules could be one of thefollowing: nucleic acid, coding nucleic acid sequence, mRNAs, antisenseRNA molecule, carbohydrate, lipid and glycolipid.

According to these pre-required conditions, a non-functional cargo toevaluate aMTD-mediated protein uptake has been selected and called asCargo A (CRA) that should be soluble and non-functional. The domain (A/a289-840; 184 A/a length) is derived from protein S (Genbank ID:CP000113.1).

4-2. Construction of Expression Vector and Preparation of RecombinantProteins

Coding sequences for recombinant proteins fused to each aMTD are clonedNde1 (5′) and Sal1 (3′) in pET-28a(+) (Novagen, Darmstadt, Germany) fromPCR-amplified DNA segments. PCR primers for the recombinant proteinsfused to aMTD and rPeptides are SEQ ID NOs: 481˜797. Structure of therecombinant proteins is displayed in FIG. 1.

The recombinant proteins were forcedly expressed in E. coli BL21 (DE3)cells grown to an OD₆₀₀ of 0.6 and induced for 2 hours with 0.7 mMisopropyl-β-D-thiogalactopyranoside (IPTG). The proteins were purifiedby Ni²⁺ affinity chromatography as directed by the supplier (Qiagen,Hilden, Germany) in natural condition. After the purification, purifiedproteins were dissolved in a physiological buffer such as DMEM medium.

TABLE 22  

  Potentially Best aMTDs (Hydrophobic, :240  Flexible, Bending,Aliphatic & Helical)  

  Random Peptides :31 No Bending Peptides (No Praline at 5 or 6 :02and/or 12) No Bending Peptides (No Central Proline) :01 Rigid Peptides(II < 50) :09 Too Much Flexible Peptides :09 Aromatic Peptides (AromaticRing Presences) :01 Hydrophobic, But Non-Aromatic Peptides :02Hydrophilic, But Non-Aliphatic Peptides :07

4-3. Expression of aMTD- or Random Peptide (rP)-Fused RecombinantProteins

Using the standardized six critical factors, 316 aMTD sequences havebeen designed. In addition, 141 rPeptides are also developed that lackone of these critical factors: no bending peptides: i) absence ofproline both in the middle and at the end of sequence or ii) absence ofproline either in the middle or at the end of sequence, rigid peptides,too much flexible peptides, aromatic peptides (aromatic ring presence),hydrophobic but non-aromatic peptides, and hydrophilic but non-aliphaticpeptides (Table 22).

These rPeptides are devised to be compared and contrasted with aMTDs inorder to analyze structure/sequence activity relationship (SAR) of eachcritical factor with regard to the peptides' intracellular deliverypotential. All peptide (aMTD or rPeptide)-containing recombinantproteins have been fused to the CRA to enhance the solubility of therecombinant proteins to be expressed, purified, prepared and analyzed.

These designed 316 aMTDs and 141 rPeptides fused to CRA were all cloned(FIG. 2) and tested for inducible expression in E. coli (FIG. 3). Out ofthese peptides, 240 aMTDs were inducibly expressed, purified andprepared in soluble form (FIG. 4). In addition, 31 rPeptides were alsoprepared as soluble form (FIG. 4).

To prepare the proteins fused to rPeptides, 60 proteins were expressedthat were 10 out of 26 rPeptides in the category of no bending peptides(Table 16); 15 out of 23 in the category of rigid peptides [instabilityindex (II)<40 [(Table 17); 19 out of 24 in the category of too muchflexible peptides (Table 18); 6 out of 27 in the category of aromaticpeptides (Table 19); 8 out of 23 in the category of hydrophobic butnon-aromatic peptides (Table 20); and 12 out of 18 in the category ofhydrophilic but non-aliphatic peptides (Table 21).

4-4. Quantitative Cell-Permeability of aMTD-Fused Recombinant Proteins

The aMTDs and rPeptides were fluorescently labeled and compared based onthe critical factors for cell-permeability by using flow cytometry andconfocal laser scanning microscopy (FIGS. 5 to 8). The cellular uptakeof the peptide-fused non-functional cargo recombinant proteins couldquantitatively be evaluated in flow cytometry, while confocal laserscanning microscopy allows intracellular uptake to be assessed visually.The analysis included recombinant proteins fused to a negative control[rP38] that has opposite characteristics (hydrophilic and aromaticsequence: YYNQSTCGGQCY) to the aMTDs (hydrophobic and aliphaticsequences). Relative cell-permeability (relative fold) of aMTDs to thenegative control was also analyzed (Table 23 and FIG. 9).

Table 23 shows Comparison Analysis of Cell-Permeability of aMTDs with aNegative Control (A: rP38).

TABLE 23 Negative Control rP38 aMTD 19.6 ± 1.6* The Average of 240 aMTDs(Best: 164.2) *Relative Fold (aMTD in Geo Mean in its comparison torP38)

Relative cell-permeability (relative fold) of aMTDs to the referenceCPPs [B: MTM12 (AAVLLPVLLAAP) (SEQ ID NO: 953), C: MTD85 (AVALLILAV)(SEQ ID NO: 954)] was also analyzed (Tables 40 and 41)

Table 24 shows Comparison Analysis of Cell-Permeability of aMTDs with aReference CPP (B: MTM12).

TABLE 24 MTM12 aMTD 13.1 ± 1.1* The Average of 240 aMTDs (Best: 109.9)*Relative Fold (aMTD in Geo Mean in its comparison to MTM12)

Table 25 shows Comparison Analysis of Cell-Permeability of aMTDs with aReference CPP (C: MTD85).

TABLE 25 MTD85 aMTD 6.6 ± 0.5* The Average of 240 aMTDs (Best: 55.5)*Relative Fold (aMTD in Geo Mean in its comparison to MTD85)

Geometric means of negative control (histidine-tagged rP38-fused CRArecombinant protein) subtracted by that of naked protein(histidine-tagged CRA protein) lacking any peptide (rP38 or aMTD) wasstandardized as relative fold of 1. Relative cell-permeability of 240aMTDs to the negative control (A type) was significantly increased by upto 164 fold, with average increase of 19.6±1.6 (Table 26-31).

TABLE 26 Sequence Proline Rigidity/ Sturctural Hydro- Relative Ratio  IDPosition Flexibility Feature pathy (Fold) Number aMTD Sequences Length(PP) (II) (AI) (GRAVY) A B C 229 899 AVVIALPAVVAP 12 7 57.3 195.0 2.4164.2 109.9 55.5 237 908 VALALAPVVVAP 12 7 57.3 195.0 2.3 150.6 100.850.9 238 910 VAALLPAVVVAP 12 6 57.3 195.0 2.3 148.5  99.4 50.2 185 810VIVLAAPALAAP 12 7 50.2 187.5 2.2 120.0  80.3 40.6 233 904 AVLAVVAPVVAP12 8 57.3 186.7 2.4 105.7  70.8 35.8  74 321 IVAVALPALAVP 12 7 50.2203.3 2.3  97.8  65.2 32.9 204 851 VLAVVLPAVALP 12 7 57.3 219.2 2.5 96.6  64.7 32.7 239 911 VALALPAVVVAP 12 6 57.3 195.0 2.3  84.8  56.828.7 205 852 VLAVAAPAVLLP 12 7 57.3 203.3 2.3  84.6  56.6 28.6 179 803AIALAVPVLALP 12 7 57.3 211.7 2.4  74.7  50.0 25.3 222 888 ILAVVAIPAAAP12 8 54.9 187.5 2.3  71.0  47.5 24.0 188 825 IVAVIVAPAVAP 12 8 43.2195.0 2.5  69.7  46.6 23.6 226 895 AIIIVVPAIAAP 12 7 50.2 211.7 2.5 60.8  40.7 20.6 227 896 AILIVVAPIAAP 12 8 50.2 211.7 2.5  57.5  38.519.4 164 727 VALAIALPAVLP 12 8 57.3 211.6 2.3  54.7  36.7 18.5 139 603VLVALAAPVIAP 12 8 57.3 203.3 2.4  54.1  36.1 18.2 200 847 LVAIVVLPAVAP12 8 50.2 219.2 2.6  50.2  33.4 16.9 189 826 LVALAAPIIAVP 12 7 41.3211.7 2.4  49.2  32.9 16.6 161 724 VAVLAVLPALAP 12 8 57.3 203.3 2.3 47.5  31.8 16.1 131 563 ALAVIVVPALAP 12 8 50.2 203.3 2.4  47.1  31.415.9 186 811 AVVLAVPALAVP 12 7 57.3 195.0 2.3  46.5  31.1 15.7 194 831IIVAVAPAAIVP 12 7 43.2 203.3 2.5  46.3  31.0 15.7 192 829 AALALVAPVIVP12 8 50.2 203.3 2.4  44.8  30.0 15.2 224 891 ILAVAAIPAALP 12 8 54.9195.8 2.2  44.7  29.9 15.1 234 905 AVIAVAPLVVAP 12 7 41.3 195.0 2.4 44.0  29.5 14.9 132 564 VAIALIVPALAP 12 8 50.2 211.7 2.4  43.6  29.114.7  34 124 IAVALPALIAAP 12 6 50.3 195.8 2.2  43.6  29.0 14.7 190 827IAAVLAAPALVP 12 8 57.3 187.5 2.2  43.0  28.8 14.6   2   2 AAAVPLLAVVVP12 5 41.3 195.0 2.4  40.9  27.2 13.8  91 385 IVAIAVPALVAP 12 7 50.2203.3 2.4  38.8  25.9 13.1 191 828 IALLAAPIIAVP 12 7 41.3 220.0 2.4 36.8  24.6 12.4 181 806 LVALAVPAAVLP 12 7 57.3 203.3 2.3  36.7  24.612.4 198 845 AAVVIAPLLAVP 12 7 41.3 203.3 2.4  35.8  24.0 12.1 218 882AIALVVPAVAVP 12 7 57.3 195.0 2.4  35.0  23.4 11.8 128 545 VVLVLAAPAAVP12 8 57.3 195.0 2.3  34.6  23.1 11.7  39 161 AVIALPALIAAP 12 6 57.3195.8 2.2  34.5  23.0 11.6 110 481 AIAIAIVPVALP 12 8 50.2 211.6 2.4 34.3  23.0 11.6 230 900 ALVAVIAPVVAP 12 8 57.3 195.0 2.4  34.3  22.911.6  53 223 AILAVPIAVVAP 12 6 57.3 203.3 2.4  33.0  22.1 11.2 187 824LIIVAAAPAVAP 12 8 50.2 187.5 2.3  32.8  21.9 11.1 130 562 ALIAAIVPALVP12 8 50.2 211.7 2.4  32.7  21.8 11.0  52 222 ALLIAPAAVIAP 12 6 57.3195.8 2.2  32.6  21.7 11.0  17  61 VAALPVLLAALP 12 5 57.3 211.7 2.3 31.2  20.8 10.5 134 582 VAVALIVPALAP 12 8 50.2 203.3 2.4  30.6  20.410.3 223 889 ILVAAAPIAALP 12 7 57.3 195.8 2.2  30.3  20.3 10.3 177 787AVALVPVIVAAP 12 6 50.2 195.0 2.4  29.3  19.6  9.9 157 703 IVAVALVPALAP12 8 50.2 203.3 2.4  29.2  19.5  9.9 158 705 IVAVALLPALAP 12 8 50.2211.7 2.4  28.6  19.1  9.7 220 885 LVAIAPAVAVLP 12 6 57.3 203.3 2.4 28.3  19.0  9.6   3   3 AALLVPAAVLAP 12 6 57.3 187.5 2.1  27.0  18.0 9.1 137 601 AAILIAVPIAAP 12 8 57.3 195.8 2.3  26.8  17.9  9.0 196 843AVLVLVAPAAAP 12 8 41.3 219.2 2.5  26.4  17.7  8.9  94 403 AAALVIPAAILP12 7 54.9 195.8 2.2  25.2  16.8  8.5 127 544 IVALIVAPAAVP 12 8 43.1203.3 2.4  23.4  15.6  7.9 121 522 ALLVIAVPAVAP 12 8 57.3 203.3 2.4 22.7  15.2  7.7

TABLE 27 Sequence Proline Rigidity/ Sturctural Hydro- Relative Ratio  IDPosition Flexibility Feature pathy (Fold) Number aMTD Sequences Length(PP) (II) (AI) (GRAVY) A B C 180 805 LVLIAAAPIALP 12 8 41.3 220.0 2.422.3 14.9 7.6 108 464 AVVILVPLAAAP 12 7 57.3 203.3 2.4 22.3 14.9 7.5  96405 LAAAVIPVAILP 12 7 54.9 211.7 2.4 22.2 14.8 7.5 168 747 VALLAIAPALAP12 8 57.3 195.8 2.2 22.0 14.8 7.5 115 501 VIVALAVPALAP 12 8 50.2 203.32.4 21.5 14.4 7.3 147 661 AAILAPIVAALP 12 6 50.2 195.8 2.2 21.4 14.3 7.2176 786 LVAIAPLAVLAP 12 6 41.3 211.7 2.4 21.2 14.2 7.2 144 625ILAAAAAPLIVP 12 8 50.2 195.8 2.2 20.9 13.9 7.0 101 442 ALAALVPAVLVP 12 757.3 203.3 2.3 20.4 13.6 6.9 240 912 VALLAPAVVVAP 12 6 57.3 195.0 2.319.9 13.3 6.7  43 165 ALAVPVALAIVP 12 5 50.2 203.3 2.4 19.8 13.2 6.7  98422 VVAILAPLLAAP 12 7 57.3 211.7 2.4 19.6 13.1 6.6 155 686 AALVAVLPVALP12 8 57.3 203.3 2.3 19.5 13.1 6.6  81 343 IVAVALPALVAP 12 7 50.2 203.32.3 19.4 12.9 6.5  76 323 IVAVALPVALAP 12 7 50.2 203.3 2.3 19.1 12.8 6.4105 461 IAAVIVPAVALP 12 7 50.2 203.3 2.4 19.0 12.7 6.4   9  21AVALLPALLAVP 12 6 57.3 211.7 2.3 18.9 12.6 6.4  95 404 LAAAVIPAAILP 12 754.9 195.8 2.2 18.9 12.6 6.4  60 261 LVLVPLLAAAAP 12 5 41.3 211.6 2.318.5 12.3 6.2 122 524 AVALIVVPALAP 12 8 50.2 203.3 2.4 18.3 12.2 6.2  55225 VAALLPAAAVLP 12 6 57.3 187.5 2.1 18.3 12.2 6.2  63 264 LAAAPVVIVIAP12 5 50.2 203.3 2.4 18.2 12.1 6.1   1   1 AAALAPVVLALP 12 6 57.3 187.52.1 17.7 11.8 6.0  88 382 AAALVIPAILAP 12 7 54.9 195.8 2.2 17.7 11.8 6.0107 463 AVAILVPLLAAP 12 7 57.3 211.7 2.4 17.6 11.7 5.9  75 322VVAIVLPALAAP 12 7 50.2 203.3 2.3 17.6 11.7 5.9 117 503 AAIIIVLPAALP 12 850.2 220.0 2.4 17.6 11.8 5.9 211 870 VLVAAVLPIAAP 12 8 41.3 203.3 2.416.6 11.1 5.6  56 241 AAAVVPVLLVAP 12 6 57.3 195.0 2.4 16.6 11.0 5.6 163726 LAVAIIAPAVAP 12 8 57.3 187.5 2.2 16.5 11.0 5.6  79 341 IVAVALPAVLAP12 7 50.2 203.3 2.3 16.4 10.9 5.5 125 542 ALALIIVPAVAP 12 8 50.2 211.62.4 16.2 10.8 5.5  83 361 AVVIVAPAVIAP 12 7 50.2 195.0 2.4 16.0 10.7 5.4 54 224 ILAAVPIALAAP 12 6 57.3 195.8 2.2 15.8 10.6 5.3  20  64AIVALPVAVLAP 12 6 50.2 203.3 2.4 15.8 10.6 5.3 111 482 ILAVAAIPVAVP 12 854.9 203.3 2.4 15.8 10.6 5.3 113 484 LAVVLAAPAIVP 12 8 50.2 203.3 2.415.6 10.4 5.3 210 868 VLVAAILPAAIP 12 8 54.9 211.7 2.4 14.9 10.0 5.0 124541 LLALIIAPAAAP 12 8 57.3 204.1 2.1 14.8  9.9 5.0 150 666 AAIAIIAPAIVP12 8 50.2 195.8 2.3 14.7  9.9 5.0 149 665 LAIVLAAPVAVP 12 8 50.2 203.32.3 14.7  9.9 5.0  84 363 AVLAVAPALIVP 12 7 50.2 203.3 2.3 14.7  9.8 4.9 57 242 AALLVPALVAAP 12 6 57.3 187.5 2.1 14.6  9.7 4.9  90 384VIVAIAPALLAP 12 7 50.2 211.6 2.4 14.0  9.4 4.7 214 877 VAIIAVPAVVAP 12 757.3 195.0 2.4 14.0  9.4 4.7 206 863 AAVVLLPIIAAP 12 7 41.3 211.7 2.413.8  9.3 4.7 123 525 ALAIVVAPVAVP 12 8 50.2 195.0 2.4 13.8  9.2 4.7 213875 AIAIVVPAVAVP 12 7 50.2 195.0 2.4 13.8  9.2 4.7  69 285 AIVLLPAAVVAP12 6 50.2 203.3 2.4 13.3  8.9 4.5  65 281 ALIVLPAAVAVP 12 6 50.2 203.32.4 13.3  8.9 4.5 209 867 ALLVVIAPLAAP 12 8 41.3 211.7 2.4 13.2  8.8 4.4172 766 IVVIAVAPAVAP 12 8 50.2 195.0 2.4 12.9  8.6 4.4  80 342VIVALAPAVLAP 12 7 50.2 203.3 2.3 12.7  8.5 4.3 217 881 AALIVVPAVAVP 12 750.2 195.0 2.4 12.7  8.5 4.3 119 505 AIIIVIAPAAAP 12 8 50.2 195.8 2.312.4  8.3 4.2

TABLE 28 Sequence Proline Rigidity/ Sturctural Hydro- Relative Ratio  IDPosition Flexibility Feature pathy (Fold) Number aMTD Sequences Length(PP) (II) (AI) (GRAVY) A B C 169 763 VAVLIAVPALAP 12 8 57.3 203.3 2.312.3 7.2 4.2 159 706 IVAVALLPAVAP 12 8 50.2 203.3 2.4 12.0 7.0 4.1 156687 AILAVALPLLAP 12 8 57.3 220.0 2.3 12.0 7.0 4.1 145 643 LALVLAAPAIVP12 8 50.2 211.6 2.4 11.8 7.9 4.0  66 282 VLAVAPALIVAP 12 6 50.2 203.32.4 11.8 7.9 4.0 126 543 LLAALIAPAALP 12 8 57.3 204.1 2.1 11.7 7.8 4.0 78 325 IVAVALPAVALP 12 7 50.2 203.3 2.3 11.7 7.8 4.0 199 846IAVAVAAPLLVP 12 8 41.3 203.3 2.4 11.7 6.8 4.0  89 383 VIVALAPALLAP 12 750.2 211.6 2.3 11.6 7.7 3.9  87 381 VVAIVLPAVAAP 12 7 50.2 195.0 2.411.5 7.7 3.9 183 808 LVVLAAAPLAVP 12 8 41.3 203.3 2.3 11.5 7.6 3.9 208865 AVLVIAVPAIAP 12 8 57.3 203.3 2.5 11.3 7.5 3.8 162 725 IAVLAVAPAVLP12 8 57.3 203.3 2.3 11.2 7.5 3.8 197 844 VVALLAPLIAAP 12 7 41.3 211.82.4 11.2 7.5 3.8 228 897 AVIVPVAIIAAP 12 5 50.2 203.3 2.5 11.2 7.5 3.8141 605 VIAAVLAPVAVP 12 8 57.3 195.0 2.4 11.0 7.4 3.7 166 744AAVVIVAPVALP 12 8 50.2 195.0 2.4 11.0 7.3 3.7  51 221 AAILAPIVALAP 12 650.2 195.8 2.2 10.9 7.3 3.7 142 622 ALIVLAAPVAVP 12 8 50.2 203.3 2.410.6 7.1 3.6  92 401 AALAVIPAAILP 12 7 54.9 195.8 2.2 10.6 7.1 3.6  77324 IVAVALPAALVP 12 7 50.2 203.3 2.3 10.3 6.9 3.5 215 878 IVALVAPAAVVP12 7 50.2 195.0 2.4 10.3 6.9 3.5  71 302 LALAPALALLAP 12 5 57.3 204.22.1 10.2 6.8 3.4 154 685 ALLVAVLPAALP 12 8 57.3 211.7 2.3 10.2 5.9 3.4201 848 AVAIVVLPAVAP 12 8 50.2 195.0 2.4 10.0 6.7 3.4 138 602VIVALAAPVLAP 12 8 50.2 203.3 2.4  9.9 5.8 3.4 178 788 AIAVAIAPVALP 12 857.3 187.5 2.3  9.8 6.6 3.3  38 145 LLAVVPAVALAP 12 6 57.3 203.3 2.3 9.5 6.3 3.2   6  11 VVALAPALAALP 12 6 57.3 187.5 2.1  9.5 6.3 3.2  35141 AVIVLPALAVAP 12 6 50.2 203.3 2.4  9.4 6.3 3.2 120 521 LAALIVVPAVAP12 8 50.2 203.3 2.4  9.4 6.3 3.2 100 425 AVVAIAPVLALP 12 7 57.3 203.32.4  9.4 6.3 3.2  86 365 AVIVVAPALLAP 12 7 50.2 203.3 2.3  9.3 6.2 3.1 62 263 ALAVIPAAAILP 12 6 54.9 195.8 2.2  9.0 6.0 3.0  82 345ALLIVAPVAVAP 12 7 50.2 203.3 2.3  8.9 5.9 3.0 203 850 LVIALAAPVALP 12 857.3 211.7 2.4  8.8 5.9 3.0  37 144 VLAIVPAVALAP 12 6 50.2 203.3 2.4 8.8 5.9 3.0 173 767 IVVAAVVPALAP 12 8 50.2 195.0 2.4  8.5 5.0 2.9  47185 AALVLPLIIAAP 12 6 41.3 220.0 2.4  8.5 5.7 2.9 202 849 AVILLAPLIAAP12 7 57.3 220.0 2.4  8.3 4.8 2.8 207 864 ALLVIAPAIAVP 12 7 57.3 211.74.8  8.2 2.4 2.8  40 162 AVVALPAALIVP 12 6 50.2 203.3 2.4  8.2 5.5 2.8 42 164 LAAVLPALLAAP 12 6 57.3 195.8 2.1  8.2 5.5 2.8 236 907VAIALAPVVVAP 12 7 57.3 195.0 2.4  8.1 5.4 2.8 103 444 LAAALVPVALVP 12 757.3 203.3 2.3  8.1 5.4 2.7 102 443 ALAALVPVALVP 12 7 57.3 203.3 2.3 8.0 5.3 2.7 231 901 ALVAVLPAVAVP 12 7 57.3 195.0 2.4  7.7 5.1 2.6 221887 VLAVAPAVAVLP 12 6 57.3 195.0 2.4  7.7 5.1 2.6 167 746 VAIIVVAPALAP12 8 50.2 203.3 2.4  7.6 4.4 2.6 232 902 ALVAPLLAVAVP 12 5 41.3 203.32.3  7.6 5.1 2.6 133 565 VAIVLVAPAVAP 12 8 50.2 195.0 2.4  7.5 5.0 2.5 59 245 AAALAPVLALVP 12 6 57.3 187.5 2.1  7.5 5.0 5.2 165 743AIAIALVPVALP 12 8 57.3 211.6 2.4  7.4 4.9 2.5 109 465 AVVILVPLAAAP 12 757.3 203.3 2.4  7.4 4.9 2.5  30 104 AVVAAPLVLALP 12 6 41.3 203.3 2.3 7.3 4.9 2.5

TABLE 29 Sequence Proline Rigidity/ Sturctural Hydro- Relative Ratio  IDPosition Flexibility Feature pathy (Fold) Number aMTD Sequences Length(PP) (II) (AI) (GRAVY) A B C 160 707 IVALAVLPAVAP 12 8 50.2 203.3 2.47.3 4.9 2.5 212 872 VLAAAVLPLVVP 12 8 41.3 219.2 2.5 7.3 4.9 2.5 135 583AVILALAPIVAP 12 8 50.2 211.6 2.4 7.3 4.8 2.4 216 879 AAIVLLPAVVVP 12 750.2 219.1 2.5 7.2 4.8 2.4 175 784 VAALPAVALVVP 12 5 57.3 195.0 2.4 7.14.7 2.4 225 893 VIAIPAILAAAP 12 5 54.9 195.8 2.3 7.0 4.7 2.4   8  13AAALVPVVALLP 12 6 57.3 203.3 2.3 7.0 4.7 2.4 184 809 LIVLAAPALAAP 12 750.2 195.8 2.2 7.0 4.7 2.4 104 445 ALAALVPALVVP 12 7 57.3 203.3 2.3 6.94.6 2.3  22  81 AALLPALAALLP 12 5 57.3 204.2 2.1 6.9 4.6 2.3 151 667LAVAIVAPALVP 12 8 50.2 203.3 2.3 6.9 4.6 2.3 235 906 AVIALAPVVVAP 12 757.3 195.0 2.4 6.8 4.6 2.3 112 483 ILAAAIIPAALP 12 8 54.9 204.1 2.2 6.84.5 2.3 114 485 AILAAIVPLAVP 12 8 50.2 211.6 2.4 6.8 4.5 2.3  97 421AAILAAPLIAVP 12 7 57.3 195.8 2.2 6.7 4.5 2.3 136 585 ALIVAIAPALVP 12 850.2 211.6 2.4 6.6 4.4 2.2  99 424 AVVVAAPVLALP 12 7 57.3 195.0 2.4 6.64.4 2.2  85 364 LVAAVAPALIVP 12 7 50.2 203.3 2.3 6.5 4.3 2.2  93 402ALAAVIPAAILP 12 7 54.9 195.8 2.2 6.4 4.3 2.2 106 462 IAAVLVPAVALP 12 757.3 203.3 2.4 6.3 4.2 2.1  64 265 VLAIAPLLAAVP 12 6 41.3 211.6 2.3 6.04.0 2.0  70 301 VIAAPVLAVLAP 12 6 57.3 203.3 2.4 6.0 4.0 2.0  45 183LLAAPVVIALAP 12 6 57.3 211.6 2.4 6.0 4.0 2.0  58 243 AAVLLPVALAAP 12 657.3 187.5 2.1 5.9 3.9 2.0 148 664 ILIAIAIPAAAP 12 8 54.9 204.1 2.3 5.73.8 1.9 174 783 IVALVPAVAIAP 12 6 50.2 203.3 2.5 5.7 3.8 1.9 116 502AIVALAVPVLAP 12 8 50.2 203.3 2.4 5.6 3.7 1.9  61 262 ALIAVPAIIVAP 12 650.2 211.6 2.4 5.5 3.7 1.9 152 683 LAIVLAAPAVLP 12 8 50.2 211.7 2.4 5.53.2 1.9 193 830 IALVAAPVALVP 12 7 57.3 203.3 2.4 5.3 3.5 1.8 170 764AVALAVLPAVVP 12 8 57.3 195.0 2.3 5.0 3.4 1.7 182 807 AVALAVPALVLP 12 757.3 203.3 2.3 5.0 3.3 1.7  46 184 LAAIVPAIIAVP 12 6 50.2 211.6 2.4 4.83.2 1.6  73 305 IALAAPILLAAP 12 6 57.3 204.2 2.2 4.8 3.2 1.6  27 101LVALAPVAAVLP 12 6 57.3 203.3 2.3 4.5 3.0 1.5  72 304 AIILAPIAAIAP 12 657.3 204.2 2.3 4.4 3.0 1.5 140 604 VALIAVAPAVVP 12 8 57.3 195.0 2.4 4.32.5 1.5 146 645 ALAVVALPAIVP 12 8 50.2 203.3 2.4 4.3 2.9 1.5  48 201LALAVPALAALP 12 6 57.3 195.8 2.1 4.2 2.8 1.4  41 163 LALVLPAALAAP 12 657.3 195.8 2.1 4.1 2.4 1.4 195 832 AVAAIVPVIVAP 12 7 43.2 195.0 2.5 4.12.7 1.4  44 182 ALIAPVVALVAP 12 6 57.3 203.3 2.4 4.0 2.7 1.4  11  23VVLVLPAAAAVP 12 6 57.3 195.0 2.4 4.0 2.6 1.3  31 105 LLALAPAALLAP 12 657.3 204.1 2.1 4.0 2.6 1.3 129 561 AAVAIVLPAVVP 12 8 50.2 195.0 2.4 3.92.6 1.3 171 765 AVALAVVPAVLP 12 8 57.3 195.0 2.3 3.8 2.2 1.3 153 684AAIVLALPAVLP 12 8 50.2 211.7 2.4 3.5 2.1 1.2  36 143 AVLAVPAVLVAP 12 657.3 195.0 2.4 3.3 2.2 1.1 118 504 LIVALAVPALAP 12 8 50.2 211.7 2.4 3.32.2 1.1  10  22 AVVLVPVLAAAP 12 6 57.3 195.0 2.4 3.1 2.1 1.1   5   5AAALLPVALVAP 12 6 57.3 187.5 2.1 3.1 2.1 1.0  67 283 AALLAPALIVAP 12 650.2 195.8 2.2 3.1 2.0 1.0  21  65 IAIVAPVVALAP 12 6 50.2 203.3 2.4 3.02.0 1.0 219 883 LAIVPAAIAALP 12 6 50.2 195.8 2.2 3.0 2.0 1.0  33 123AAIIVPAALLAP 12 6 50.2 195.8 2.2 2.9 2.0 1.0

TABLE 30 Sequence Proline Rigidity/ Sturctural Hydro- Relative Ratio  IDPosition Flexibility Feature pathy (Fold) Number aMTD Sequences Length(PP) (II) (AI) (GRAVY) A B C  68 284 ALIAPAVALIVP 12 5 50.2 211.7 2.42.8 1.8 0.9  50 205 ALALVPAIAALP 12 6 57.3 195.8 2.2 2.6 1.7 0.9  14  42VAALPVVAVVAP 12 5 57.3 186.7 2.4 2.5 1.7 0.8  32 121 AIVALPAIALAP 12 650.2 195.8 2.2 2.5 1.7 0.8  13  25 IVAVAPALVALP 12 6 50.2 203.3 2.4 2.41.6 0.8  12  24 IALAAPALIVAP 12 6 50.2 195.8 2.2 2.3 1.6 0.8  49 204LIAALPAVAALP 12 6 57.3 195.8 2.2 2.2 1.5 0.8   7  12 LLAAVPAVLLAP 12 657.3 211.7 2.3 2.2 1.5 0.7  15  43 LLAAPLVVAAVP 12 5 41.3 187.5 2.1 2.11.4 0.7  29 103 ALIAAPILALAP 12 6 57.3 204.2 2.2 2.1 1.4 0.7  23  82AVVLAPVAAVLP 12 6 57.3 195.0 2.4 2.1 1.4 0.7   4   4 ALALLPVAALAP 12 657.3 195.8 2.1 2.0 1.3 0.7  26  85 LLVLPAAALAAP 12 5 57.3 195.8 2.1 1.91.3 0.7  19  63 AALLVPALVAVP 12 6 57.3 203.3 2.3 1.9 1.3 0.7  16  44ALAVPVALLVAP 12 5 57.3 203.3 2.3 1.6 1.1 0.5  25  84 AAVAAPLLLALP 12 641.3 195.8 2.1 1.5 1.0 0.5  18  62 VALLAPVALAVP 12 6 57.3 203.3 2.3 1.40.9 0.5  24  83 LAVAAPLALALP 12 6 41.3 195.8 2.1 1.4 0.9 0.5  28 102LALAPAALALLP 12 5 57.3 204.2 2.1 1.4 0.9 0.5 143 623 VAAAIALPAIVP 12 850.2 187.5 2.3 0.8 0.6 0.3 19.6 ± 13.1 ± 6.6 ± 1.6 1.1 0.5Moreover, compared to reference CPPs (B type: MTM12 and C type: MTD85),novel 240 aMTDs averaged of 13±1.1 (maximum 109.9) and 6.6±0.5 (maximum55.5) fold higher cell-permeability, respectively (Tables 26-31).

TABLE 31 Negative Control rP38 MTM12 MTD85 aMTD 19.6 ± 1.6* 13.1 ± 1.1*6.6 ± 0.5* The Average of 240 aMTDs (Best: 164.2) (Best: 109.9) (Best:55.5) *Relative Fold (aMTD in Geo Mean in its comparison to rP38, MTM12or MTD85)

In addition, cell-permeability of 31 rPeptides has been compared withthat of 240 aMTDs (0.3±0.04; Tables 32 and 33).

TABLE 32 Number Proline Rigidity/ Sturctural Hydro- Relative SEQ IDPosition Flexibility Feature pathy Ratio To NOS) ID Sequence Length (PP)(II) (AI) (GRAVY) aMTD AVE 907 692 PAPLPPVVILAV 12 1, 3, 5, 6 105.5186.7  1.8 0.74 895  26 AAIAKLAAPLAIV 12 8  18.1 204.2  2.5 0.65 891 113PVAVALLIAVPP 12 1, 11, 12  57.3 195.0  2.1 0.61 897 466 IIAAAAPLAIIP 127, 12  22.8 204.2  2.3 0.52 898 167 VAIAIPAALAIP 12 6, 12  20.4 195.8 2.3 0.50 936  97 ALLAAPPALLAL 12 6, 7  57.3 204.2  2.1 0.41 909 390VPLLVPVVPVVP 12 2, 6, 9, 12 105.4 210.0  2.2 0.41 900 426 AAALAIPLAIIP12 7, 12   4.37 204.2  2.2 0.40 937 241 ALIVAPALMALP 12 6, 12  60.5187.5  2.2 0.33 913  68 VAPVLPAAPLVP 12 3, 6, 9, 12 105.5 162.5  1.60.32 941  39 CYNTSPCTGCCY 12 6  52.5   0.0  0.0 0.29 888 934LILAPAAVVAAA 12 5  57.5 195.8  2.5 0.28 916 938 VPVLLPVVVPVP 122, 6, 10, 12 121.5 210.0  2.2 0.28 917 329 LPVLVPVVPVVP 12 2, 6, 9, 12121.5 210.0  2.2 0.23 901 606 AAAIAAIPIIIP 12 8, 12   4.4 204.2  2.40.20 918  49 VVPAAPAVPVVP 12 3, 6, 9, 12 121.5 145.8  1.7 0.18 944 139TGSTNSPTCTST 12 7  53.4   0.0 -0.7 0.17 919 772 LPVAPVIPIIVP 122, 5, 8, 12  79.9 210.8  2.1 0.16 931 921 IWWFVVLPLVVP 12 8, 12  41.3194.2  2.2 0.14 902  66 AGVLGGPIMGVP 12 7, 12  35.5 121.7  1.3 0.13 922693 AAPVLPVAVPIV 12 3, 6, 10  82.3 186.7  2.1 0.13 945  18 NYCCTPTTNGQS12 6  47.9   0.0 -0.9 0.10 890  16 NNSCITYINGSQ 12 None  47.4   0.0 -1.40.08 904 227 LAAIVPIAAAVP 12 6, 12  34.2 187.5  2.2 0.08 905  17GGCSAPQTTCSN 12 6  51.6   8.3 -0.5 0.08 906  67 LDAEVPLADDVP 12 6, 12 34.2 130.0  0.3 0.08 947 635 GSTGGSQQNNQY 12 None  31.9   0.0 -1.9 0.07924  29 VLPPLPVLPVLP 12 3, 4, 6, 9, 12 121.5 202.5  1.7 0.07 949  57QNNCNTSSQGGG 12 None  52.4   0.0 -1.6 0.06 951 700 GTSNTCQSNQNS 12 None 19.1   0.0 -1.6 0.05 952  38 YYNQSTCGGQCY 12 ND  53.8   0.0 -1.0 0.05AVE 0.3 ± 0.04

TABLE 33 Relative Ratio to aMTD AVE* rPeptide 0.3 ± 0.04 The Average of31 aMTDs *Out of 240 aMTDs, average relative fold of aMTD had been 19.6fold compared to type A (rP38).

In summary, relative cell-permeability of aMTDs has shown maximum of164.0, 109.9 and 55.5 fold higher to rP38, MTM12 and MTD85,respectively. In average of total 240 aMTD sequences, 19.6±1.6, 13.1±1.1and 6.6±0.5 fold higher cell-permeability are shown to the rP38, MTM12and MTD85, respectively (Tables 26-31). Relative cell-permeability ofnegative control (rP38) to the 240 aMTDs is only 0.3±0.04 fold.

4-5. Intracellular Delivery and Localization of aMTD-Fused RecombinantProteins

Recombinant proteins fused to the aMTDs were tested to determine theirintracellular delivery and localization by laser scanning confocalmicroscopy with a negative control (rP38) and previous published CPPs(MTM12 and MTD85) as the positive control references. NIH3T3 cells wereexposed to 10 μM of FITC-labeled protein for 1 hour at 37, and nucleiwere counterstained with DAPI. Then, cells were examined by confocallaser scanning microscopy (FIG. 7). Recombinant proteins fused to aMTDsclearly display intracellular delivery and cytoplasmic localization(FIG. 7) that are typically higher than the reference CPPs (MTM12 andMTD85). The rP38-fused recombinant protein did not show internalizedfluorescence signal (FIG. 7a ). In addition, as seen in FIG. 8,rPeptides (his-tagged CRA recombinant proteins fused to each rPeptide)display lower- or non-cell-permeability.

4-6. Summary of Quantitative and Visual Cell-Permeability of NewlyDeveloped aMTDs

Histidine-tagged aMTD-fused cargo recombinant proteins have been greatlyenhanced in their solubility and yield. Thus, FITC-conjugatedrecombinant proteins have also been tested to quantitate and visualizeintracellular localization of the proteins and demonstrated highercell-permeability compared to the reference CPPs.

In the previous studies using the hydrophobic signal-sequence-derivedCPPs-MTS/MTM or MTDs, 17 published sequences have been identified andanalyzed in various characteristics such as length, molecular weight, pIvalue, bending potential, rigidity, flexibility, structural feature,hydropathy, amino acid residue and composition, and secondary structureof the peptides. Based on these analytical data of the sequences, novelartificial and non-natural peptide sequences designated as advanced MTDs(aMTDs) have been invented and determined their functional activity inintracellular delivery potential with aMTD-fused recombinant proteins.

aMTD-fused recombinant proteins have promoted the ability of proteintransduction into the cells compared to the recombinant proteinscontaining rPeptides and/or reference hydrophobic CPPs (MTM12 andMTD85). According to the results, it has been demonstrated that criticalfactors of cell-penetrating peptide sequences play a major role todetermine peptide-mediated intracellular delivery by penetrating plasmamembrane. In addition, cell-permeability can considerably be improved byfollowing the rational that all satisfy the critical factors.

5. Structure/Sequence Activity Relationship (SAR) of aMTDs on DeliveryPotential

After determining the cell-permeability of novel aMTDs,structure/sequence activity relationship (SAR) has been analyzed foreach critical factor in selected some of and all of novel aMTDs (FIGS.13 to 16 and Table 34).

TABLE 34 Rank of Rigidity/ Sturctural Delivery Flexibility FeatureHydropathy Relative Ratio (Fold) Amino Acid Composition Potential (II)(AI) (GRAVY) A B C A V I L  1~10 55.9 199.2 2.3 112.7 75.5 38.1 4.0 3.50.4 2.1 11~20 51.2 205.8 2.4 56.2 37.6 19.0 4.0 2.7 1.7 1.6 21~30 49.1199.2 2.3 43.6 28.9 14.6 4.3 2.7 1.4 1.6 31~40 52.7 201.0 2.4 34.8 23.311.8 4.2 2.7 1.5 1.6 41~50 53.8 201.9 2.3 30.0 20.0 10.1 4.3 2.3 1.1 2.351~60 51.5 205.2 2.4 23.5 15.7 7.9 4.4 2.1 1.5 2.0 222~231 52.2 197.22.3 2.2 1.5 0.8 4.5 2.1 1.0 2.4 232~241 54.1 199.7 2.2 1.7 1.2 0.6 4.61.7 0.2 3.5

5-1. Proline Position:

In regards to the bending potential (proline position: PP), aMTDs withits proline at 7′ or 8′ amino acid in their sequences have much highercell-permeability compared to the sequences in which their prolineposition is at 5′ or 6′ (FIGS. 14a and 15a ).

5-2. Hydropathy:

In addition, when the aMTDs have GRAVY (Grand Average of Hydropathy)ranging in 2.1-2.2, these sequences display relatively lowercell-permeability, while the aMTDs with 2.3-2.6 GRAVY are shownsignificantly higher one (FIGS. 14b and 15b ).

5-3. rPeptide SAR:

To the SAR of aMTDs, rPeptides have shown similar SAR correlations inthe cell-permeability, pertaining to their proline position (PP) andhydropathy (GRAVY). These results confirms that rPeptides with highGRAVY (2.4˜2.6) have better cell-permeability (FIG. 16).

5-4. Analysis of Amino Acid Composition:

In addition to proline position and hydropathy, the difference of aminoacid composition is also analyzed. Since aMTDs are designed based oncritical factors, each aMTD-fused recombinant protein has equally twoproline sequences in the composition. Other hydrophobic and aliphaticamino acids—alanine, isoleucine, leucine and valine—are combined to formthe rest of aMTD peptide sequences.

Alanine: In the composition of amino acids, the result does not show asignificant difference by the number of alanine in terms of the aMTD'sdelivery potential because all of the aMTDs have three to five alanines.In the sequences, however, four alanine compositions show the mosteffective delivery potential (geometric mean) (FIG. 13a ).

Leucine and Isoleucine: Also, the compositions of isoleucine and leucinein the aMTD sequences show inverse relationship between the number ofamino acid (I and L) and delivery potential of aMTDs. Lower number ofisoleucine and leucine in the sequences tends to have higher deliverypotential (geometric mean) (FIGS. 13a and 13b ).

Valine: Conversely, the composition of valine of aMTD sequences showspositive correlation with their cell-permeability. When the number ofvaline in the sequence is low, the delivery potential of aMTD is alsorelatively low (FIG. 13b ).

Ten aMTDs having the highest cell-permeability are selected (averagegeometric mean: 2584±126). Their average number of valine in thesequences is 3.5; 10 aMTDs having relatively low cell-permeability(average geometric mean: 80±4) had average of 1.9 valine amino acids.The average number of valine in the sequences is lowered as theircell-permeability is also lowered as shown in FIG. 13b . Compared tohigher cell-permeable aMTDs group, lower sequences had average of 1.9 intheir valine composition. Therefore, to obtain high cell-permeablesequence, an average of 2-4 valines should be composed in the sequence.

5-5. Conclusion of SAR Analysis:

As seen in FIG. 15, all 240 aMTDs have been examined for theseassociation of the cell-permeability and the critical factors: bendingpotential (PP), rigidity/flexibility (II), structure feature (AI), andhydropathy (GRAVY), amino acid length and composition. Through thisanalysis, cell-permeability of aMTDs tends to be lower when theircentral proline position is at 5′ or 6′ and GRAVY is 2.1 or lower (FIG.15). Moreover, after investigating 10 higher and 10 lower cell-permeableaMTDs, these trends are clearly shown to confirm the association ofcell-permeability with the central proline position and hydropathy.

6. Experimental Confirmation of Index Range/Feature of Critical Factors

The range and feature of five out of six critical factors have beenempirically and experimentally determined that are also included in theindex range and feature of the critical factors initially proposedbefore conducting the experiments and SAR analysis. In terms of indexrange and feature of critical factors of newly developed 240 aMTDs, thebending potential (proline position: PP), rigidity/flexibility(Instability Index: II), structural feature (Aliphatic Index: AI),hydropathy (GRAVY), amino acid length and composition are all within thecharacteristics of the critical factors derived from analysis ofreference hydrophobic CPPs.

Therefore, our hypothesis to design and develop new hydrophobic CPPsequences as advanced MTDs is empirically and experimentally proved anddemonstrated that critical factor-based new aMTD rational design iscorrect.

TABLE 35 Summarized Critical Factors of aMTD Newly Designed Analysis ofCPPs Experimental Results Critical Factor Range Range Bending PotentialProline presences in Proline presences in (Proline Position: PP) themiddle (5′, 6′, 7′ the middle (5′, 6′, 7′ or 8′) and at the or 8′) andat the end of peptides end of peptides Rigidity/Flexibility 40-6041.3-57.3 (Instability Index: II) Structural Feature 180-220 187.5-220.0(Aliphatic Index: AI) Hydropathy 2.1-2.6 2.2-2.6 (Grand Average ofHydropathy GRAVY) Length  9-13 12 (Number of Amino Acid) Amino acidComposition A, V, I, L, P A, V, I, L, P

7. Discovery and Development of Protein-Based New Biotherapeutics withMITT Enabled by aMTDs for Protein Therapy

Total of 240 aMTD sequences have been designed and developed based onthe critical factors. Quantitative and visual cell-permeability of 240aMTDs (hydrophobic, flexible, bending, aliphatic and 12 a/a-lengthpeptides) are all practically determined.

To measure the cell-permeability of aMTDs, rPeptides have also beendesigned and tested. As seen in FIGS. 13 to 15, there are vividassociation of cell-permeability and the critical factors of thepeptides. Out of these critical factors, we are able to configure thatthe most effective cell-permeable aMTDs have the amino acid length of12; composition of A, V, L, I and P; multiple proline located at either7′ or 8′ and at the end (12′); instability index ranged of 41.3-57.3;aliphatic index ranged of 187.5-220.0; and hydropathy (GRAVY) ranged of2.2-2.6.

These examined critical factors are within the range that we have setfor our critical factors; therefore, we are able to confirm that theaMTDs that satisfy these critical factors have relatively highcell-permeability and much higher intracellular delivery potentialcompared to reference hydrophobic CPPs reported during the past twodecades.

It has been widely evident that many human diseases are caused byproteins with deficiency or over-expression that causes mutations suchas gain-of-function or loss-of-function. If biologically active proteinscould be delivered for replacing abnormal proteins within a short timeframe, possibly within an hour or two, in a quantitative manner, thedosage may be regulated depending on when and how proteins may beneeded. By significantly improving the solubility and yield of novelaMTD in this invention (Table 31), one could expect its practicalpotential as an agent to effectively deliver therapeutic macromoleculessuch as proteins, peptides, nucleic acids, and other chemical compoundsinto live cells as well as live mammals including human. Therefore,newly developed MITT utilizing the pool (240) of novel aMTDs can be usedas a platform technology for discovery and development of protein-basedbiotherapeutics to apprehend intracellular protein therapy afterdetermining the optimal cargo-aMTD relationship.

The following examples are presented to aid practitioners of theinvention, to provide experimental support for the invention, and toprovide model protocols. In no way are these examples to be understoodto limit the invention.

Example 1. Development of Novel Advanced Macromolecule TransductionDomain (aMTD)

H-regions of signal sequences (HRSP)-derived CPPs (MTS/MTM and MTD) donot have a common sequence, a sequence motif, and/or a common structuralhomologous feature. In this invention, the aim is to develop improvedhydrophobic CPPs formatted in the common sequence and structural motifthat satisfy newly determined ‘critical factors’ to have a ‘commonfunction,’ to facilitate protein translocation across the plasmamembrane with similar mechanism to the analyzed CPPs.

The structural motif as follows:

In Table 9, universal common sequence/structural motif is provided asfollows. The amino acid length of the peptides in this invention rangesfrom 9 to 13 amino acids, mostly 12 amino acids, and their bendingpotentials are dependent with the presence and location of proline inthe middle of sequence (at 5′, 6′, 7′ or 8′ amino acid) and at the endof peptide (at 12′) for recombinant protein bending. Instability index(II) for rigidity/flexibility of aMTDs is II<40, grand average ofhydropathy (GRAVY) for hydropathy is around 2.2, and aliphatic index(AI) for structural features is around 200 (Table 9). Based on thesestandardized critical factors, new hydrophobic peptide sequences, namelyadvanced macromolecule transduction domain peptides (aMTDs), in thisinvention have been developed and summarized in Tables 10 to 15.

Example 2. Construction of Expression Vectors for Recombinant ProteinsFused to aMTDs

Our newly developed technology has enabled us to expand the method formaking cell-permeable recombinant proteins. The expression vectors weredesigned for histidine-tagged CRA proteins fused with aMTDs orrPeptides. To construct expression vectors for recombinant proteins,polymerase chain reaction (PCR) had been devised to amplify eachdesigned aMTD or rPeptide fused to CRA.

The PCR reactions (100 ng genomic DNA, 10 pmol each primer, each 0.2 mMdNTP mixture, 1× reaction buffer and 2.5 U Pfu(+) DNA polymerase (Doctorprotein, Korea) was digested on the restriction enzyme site between NdeI (5′) and Sal I (3′) involving 35 cycles of denaturation (95° C.),annealing (62° C.), and extension (72° C.) for 30 seconds each. For thelast extension cycle, the PCR reactions remained for 5 minutes at 72° C.Then, they were cloned into the site of pET-28a(+) vectors (Novagen,Darmstadt, Germany). DNA ligation was performed using T4 DNA ligase at4° C. overnight. These plasmids were mixed with competent cells of E.coli DH5-alpha strain on the ice for 10 minutes. This mixture was placedon the ice for 2 minutes after it was heat shocked in the water bath at42° C. for 90 seconds. Then, the mixture added with LB broth media wasrecovered in 37° C. shaking incubator for 1 hour. Transformant wasplated on LB broth agar plate with kanamycin (50 μg/mL) (Biopure,Johnson City, Tenn., USA) before incubating at 37° C. overnight. From asingle colony, plasmid DNA was extracted, and after the digestion of NdeI and Sal I restriction enzymes, digested DNA was confirmed at 645 bp byusing 1.2% agarose gels electrophoresis (FIG. 2). PCR primers for theCRA recombinant proteins fused to aMTD and random peptides (rPeptide)are summarized in Tables 23 to 30. Amino acid sequences of aMTD andrPeptide primers are shown in Tables 31 to 38.

Example 3. Inducible Expression, Purification and Preparation ofRecombinant Proteins Fused to aMTDs and rPeptides

To express recombinant proteins, pET-28a(+) vectors for the expressionof CRA proteins fused to a negative control [rPeptide 38 (rP38)],reference hydrophobic CPPs (MTM₁₂ and MTD₈₅) and aMTDs were transformedin E. coli BL21 (DE3) strains. Cells were grown at 37° C. in LB mediumcontaining kanamycin (50 μg/ml) with a vigorous shaking and induced atOD₆₀₀=0.6 by adding 0.7 mM IPTG (Biopure) for 2 hours at 37° C. Inducedrecombinant proteins were loaded on 15% SDS-PAGE gel and stained withCoomassie Brilliant Blue (InstantBlue, Expedeon, Novexin, UK) (FIG. 3).

The E. coli cultures were harvested by centrifugation at 5,000×rpm for10 minutes, and the supernatant was discarded. The pellet wasre-suspended in the lysis buffer (50 mM NaH₂PO₄, 10 mM Imidazol, 300 mMNaCl, pH 8.0). The cell lysates were sonicated on ice using a sonicator(Sonics and Materials, Inc., Newtown, Conn., USA) equipped with a probe.After centrifuging the cell lysates at 5,000×rpm for 10 minutes topellet the cellular debris, the supernatant was incubated with lysisbuffer-equilibrated Ni-NTA resin (Qiagen, Hilden, Germany) gently byopen-column system (Bio-rad, Hercules, Calif., USA). After washingprotein-bound resin with 200 ml wash buffer (50 mM NaH₂PO₄, 20 mMImidazol, 300 mM NaCl, pH 8.0), the bounded proteins were eluted withelution buffer (50 mM NaH₂PO₄, 250 mM Imidazol, 300 mM NaCl, pH 8.0).

Recombinant proteins purified under natural condition were analyzed on15% SDS-PAGE gel and stained with Coomassie Brilliant Blue (FIG. 4). Allof the recombinant proteins were dialyzed for 8 hours and overnightagainst physiological buffer, a 1:1 mixture of cell culture medium(Dulbecco's Modified Eagle's Medium: DMEM, Hyclone, Logan, Utah, USA)and Dulbecco's phosphate buffered saline (DPBS, Gibco, Grand Island,N.Y., USA). From 316 aMTDs and 141 rPeptides cloned, 240 aMTD- and 31rPeptide-fused recombinant proteins were induced, purified, prepared andanalyzed for their cell-permeability.

Example 4. Determination of Quantitative Cell-Permeability ofRecombinant Proteins

For quantitative cell-permeability, the aMTD- or rPeptide-fusedrecombinant proteins were conjugated to fluorescein isothiocyanate(FITC) according to the manufacturer's instructions (Sigma-Aldrich, St.Louis, Mo., USA). RAW 264.7 cells were treated with 10 μM FITC-labeledrecombinant proteins for 1 hour at 37° C., washed three times with coldPBS, treated with 0.25% tripsin/EDTA (Sigma-Aldrich, St. Louis, Mo.) for20 minutes at 37° C. to remove cell-surface bound proteins.Cell-permeability of these recombinant proteins were analyzed by flowcytometry (Guava, Millipore, Darmstadt, Germany) using the FlowJocytometric analysis software (FIGS. 5 to 6). The relativecell-permeability of aMTDs were measured and compared with the negativecontrol (rP38) and reference hydrophobic CPPs (MTM12 and MTD85) (Table31).

Example 5. Determination of Cell-Permeability and IntracellularLocalization of Recombinant Proteins

For a visual reference of cell-permeability, NIH3T3 cells were culturedfor 24 hours on coverslip in 24-wells chamber slides, treated with 10 μMFITC-conjugated recombinant proteins for 1 hour at 37° C., and washedthree times with cold PBS. Treated cells were fixed in 4%paraformaldehyde (PFA, Junsei, Tokyo, Japan) for 10 minutes at roomtemperature, washed three times with PBS, and mounted with VECTASHIELDMounting Medium (Vector laboratories, Burlingame, Calif., USA), andcounter stained with DAPI (4′,6-diamidino-2-phenylindole). Theintracellular localization of the fluorescent signal was determined byconfocal laser scanning microscopy (LSM700, Zeiss, Germany; FIGS. 7 and8).

Example 6-1. Cloning of aMTD/SD-Fused SOCS3 Recombinant Protein

Full-length cDNA for human SOCS3 (SEQ ID NO: 815) was purchased fromOrigene (USA). Histidine-tagged human SOCS3 proteins were constructed byamplifying the SOCS3 cDNA (225 amino acids) using primers for aMTD fusedto SOCS3 cargo. The PCR reactions (100 ng genomic DNA, 10 pmol eachprimer, each 0.2 mM dNTP mixture, 1× reaction buffer and 2.5 U Pfu(+)DNA polymerase (Doctor protein, Korea)) were digested on the restrictionenzyme site between Nde I (5′) and Sal I (3′) involving 35 cycles ofdenaturing (95° C.), annealing (62° C.), and extending (72° C.) for 45sec each. For the last extension cycle, the PCR reactions remained for10 min at 72° C. The PCR products were subcloned into 6×His expressionvector, pET-28a(+) (Novagen, Darmstadt, Germany). Coding sequence forSDA or SDB fused to C terminus of his-tagged aMTD-SOCS3 was cloned atBamHI (5′) and Sal1 (3′) in pET-28a(+) from PCR-amplified DNA segmentsand confirmed by DNA sequence analysis of the resulting plasmids.

TABLE 36 Recombinant Cargo SD Protein 5′ Primers 3′ Primers SOCS3 — HS35′-GGAATTCCATATGGTCA 5′-CCCGGATCCTTAAAGC CCCACAGCAAGTTTCCCGCGGGGCATCGTACTGGTCC CGCC-3′ AGGAA-3′ (SEQ ID NO: 955) (SEQ ID NO: 956) —HM₁₆₅S3 5′-GGAATTCCATATGGCGCT 5′-CCCGGATCCTTAAAG GGCGGTGCCGGTGGCGCTCGGGGCATCGTACTGGT GGCGATTGTGCCGGTCACC CCAGGAA-3′ CACAGCAAGTTTC-3′(SEQ ID NO: 958) (SEQ ID NO: 957) A HM₁₆₅S3A 5′-GGAATTCCATATGGCGC5′-CGCGTCGACTTACCTCG TGGCGGTGCCGGTGGCGC GCTGCACCGG CACGGAGTGGCGATTGTGCCGGTCAC ATGAC-3′ CCACAGCAAGTTTC-3′ (SEQ ID NO: 960)(SEQ ID NO: 959) B HM₁₆₅S3B 5′-GGAATTCCATATGGCGCT 5′-CGCGTCGACTTAAAGGGGCGGTGCCGGTGGCGCT GTTTCCGAAGGCTTGGCT GGCGATTGTGCCGGTCACC ATCTT-3′CACAGCAAGTTTC-3′ (SEQ ID NO: 962) (SEQ ID NO: 961) C HM₁₆₅S3C5′-GGAATTCCATATGGCGC 5′-GCGTCGACTTAGGC TGGCGGTGCCGGTGGCGCTCAGGTTAGCGTCGAG-3′ GGCGATTGTGCCGGTCACC (SEQ ID NO: 964) CACAGCAAGTTTC-3′(SEQ ID NO: 963) D HM₁₆₅S3D 5′-GGAATTCCATATGGCGC 5′-GCGTCGACTTATTTTTGGCGGTGCCGGTGGCGC TTCTCGGACAGATA-3′ TGGCGATTGTGCCGGTCAC(SEQ ID NO: 966) CCACAGCAAGTTTC-3′ (SEQ ID NO: 965) E HM₁₆₅S3E5′-GGAATTCCATATGGCGC 5′-ACGCGTCGACTTAA TGGCGGTGCCGGTGGCGCCCTCCAATCTGTTCGCG TGGCGATTGTGCCGGTCA GTGAGCCTC-3′ CCCACAGCAAGTTTC-3′(SEQ ID NO: 968) (SEQ ID NO: 967)

Example 6-2. Preparation of aMTD/SD-Fused SOCS3 Recombinant Protein

To determine a stable structure of the cell-permeable aMTD/SD-fusedSOCS3 recombinant protein, a pET-28a(+) vector and an E. coliBL21-CodonPlus (DE3)-RIL were subjected to the following experiment.

Each of the recombinant expression vectors, HS3, HMS3, HMS3A, HMS3B,HMS3C, HMS3D, and HMS3E prepared in example 6-1 was transformed into E.coli BL21 CodonPlus(DE3)-RIL by a heat shock method, and then 600 ul ofeach was incubated in an LB medium (Biopure, Johnson City, Tenn., USA)containing 50 μg/ml of kanamycin at 37° C. for 1 hour. Thereafter, therecombinant protein gene-introduced E. coli was inoculated in 7 ml of LBmedium, and then incubated at 37° C. overnight. The E. coli wasinoculated in 700 ml of LB medium and incubated at 37° C. until OD₆₀₀reached 0.6. To this culture medium, 0.6 mM ofisopropyl-β-D-thiogalactoside (IPTG, Gen Depot, USA) was added as aprotein expression inducer, followed by further incubation at 37° C. for3 hours. This culture medium was centrifuged at 4° C. and 8,000 rpm for10 minutes and a supernatant was discarded to recover a cell pellet. Thecell pellet thus recovered was suspended in a lysis buffer (100 mMNaH₂PO₄, 10 mM Tris-HCl, 8 M Urea, pH 8.0), and cells were disrupted bysonication (on/off time: 30 sec/30 sec, on time 2 hours, amplify 40%),and centrifuged at 15,000 rpm for 30 min to obtain a soluble fractionand an insoluble fraction.

This insoluble fraction was suspended in a denature lysis buffer (8 MUrea, 10 mM Tris, 100 mM Sodium phosphate) and purified by Ni²⁺ affinitychromatography as directed by the supplier(Qiagen, Hilden, Germany) andrefolded by dialyzing with a refolding buffer (0.55 M guanidine HCl,0.44 M L-arginine, 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 100 mM NDSB,2 mM reduced glutathione, and 0.2 mM oxidized glutathione). Afterpurification, the proteins were put in a SnakeSkin Dialysis Tubing bag(pore size: 10000 mw, Thermo scientific, USA) and then they weredialyzed by physiological buffer (DMEM). The strain lysate where proteinexpression was not induced, the strain lysate where protein expressionwas induced by addition of IPTG, and purified proteins were loaded onSDS-PAGE to analyze protein expression characteristics and expressionlevels (FIGS. 19 and 20).

As shown in FIG. 19, it was confirmed that SOCS3 recombinant proteinsshowed high expression levels in the BL21CodonPlus(DE3)-RIL strain.SOCS3 recombinant proteins containing aMTD₁₆₅ and solubilization domain(HM₁₆₅S3A and HM₁₆₅S3B) had little tendency to precipitate whereasrecombinant SOCS3 proteins lacking a solubilization domain (HM₁₆₅S3) orlacking an aMTD and a SD (HS3) were largely insoluble. Solubility ofaMTD/SD-fused SOCS3 proteins was scored on a 5 point scale compared withthat of SOCS3 proteins lacking the solubilization domain.

Example 6-3. Determination of Solubility/Yield of aMTD/SD-Fused SOCS3Recombinant Proteins According to SD Type

To determine aMTD/SD-fused SOCS3 recombinant proteins having optimalcell-permeability, solubilization domains were replaced in the samemanner as in Example 6-2 to prepare 5 kinds of aMTD/SD-fused SOCS3recombinant proteins, and their solubility/yield were measured (FIG.19).

As shown in FIG. 19, it was confirmed that the aMTD/SD-fused SOCS3recombinant protein prepared by fusing with SDB among the different SDsshowed the highest solubility/yield. Therefore, the SDB-fused iCP-SOCS3recombinant protein was used in the subsequent experiment.

Example 6-4. Comparison Between aMTD/SD-Fused SOCS3 Recombinant Proteinand Basic CPP/SD-Fused SOCS3 Recombinant Protein

To compare the solubility/yield, cell/tissue-permeability, mechanism ofcytopermeability of aMTD/SD-fused SOCS3 recombinant proteins to those ofconventional basic CPP/SD-fused SOCS3 recombinant proteins, cloning,preparation, and measurement of solubility/yield of the basicCPP/SD-fused SOCS3 recombinant proteins were performed in the samemanner as in Examples 6-1 to 6-3 except for a known basic CPP (TAT orPolyR) being used instead of aMTD. Sequences of amino acids andnucleotides of basic CPP, and the primers used in this example are shownin FIG. 97.

The solubility/yield of aMTD165/SD-fused SOCS3 recombinant proteins wasmuch higher than that of TAT/SD-fused SOCS3 or PolyR/SD-fused SOCS3recombinant proteins (FIG. 98).

Example 7-1. Cell-Permeability Test

To examine cell-permeability of SOCS3 recombinant protein, SOCS3recombinant proteins were conjugated to 5/6-fluorescein isothiocyanate(FITC). RAW 264.7 (KCLB, Seoul, South Korea) (FIG. 20) or NIH3T3 cells(KCLB, Seoul, South Korea) (FIG. 21) were treated with 10 μMFITC-labeled SOCS3 recombinant proteins and cultivated for lhr at 37° C.

In this regard, RAW 264.7 cells were cultured in a DMEM mediumcontaining 10% fetal bovine serum (FBS, Hyclone, USA) and 500 mg/ml of1% penicillin/streptomycin (Hyclone, USA).

After cultivation, the cells were washed three times with ice-cold PBS(Phosphate-buffered saline, Hyclone, USA) and treated with proteinase K(10 μg/mL, SIGMA, USA) to remove surface-bound proteins, andinternalized proteins were measured by flow cytometry (FlowJo cytometricanalysis software, Guava, Millipore, Darmstadt, Germany). Untreatedcells (gray) and equimolar concentration of unconjugated FITC (FITConly, green)-treated cells were served as control (FIG. 20). Each ofNIH3T3 cells was incubated for 1 hour at 37° C. with 10 μM FITC-labeledSOCS3 protein. For nuclear staining, a mixture of VECTASHIELD MountingMedium (Vector Laboratories, Burlingame, Calif.) and DAPI(4′,6-diamidino-2-phenylindole) was added to NIH3T3 cells, andvisualized using a confocal laser microscope (LSM700, Zeiss, Germany)(FIG. 21).

As shown in FIGS. 20 and 21, SOCS3 recombinant proteins containingaMTD₁₆₅ (HM₁₆₅S3, HM₁₆₅S3A and HM₁₆₅S3B) efficiently entered the cells(FIGS. 20 and 21) and were localized to various extents in cytoplasm(FIG. 21). In contrast, SOCS3 protein containing non-aMTD (HS3) did notappear to enter cells. While all SOCS3 proteins containing aMTD₁₆₅transduced into the cells, HM₁₆₅S3B displayed more uniform cellulardistribution, and protein uptake of HM₁₆₅S3B was also very efficient.

Example 7-1-2. Comparison Between aMTD/SD-Fused SOCS3 RecombinantProtein and Basic CPP/SD-Fused SOCS3 Recombinant Protein

The cell-permeability of basic CPP/SD-fused SOCS3 recombinant proteinswas assessed by the same method as used in Example 7-1 except for aknown basic CPP (TAT or PolyR) being used instead of aMTD. The resultsof the assessment were shown in FIG. 99.

According to the results, all recombinant proteins exhibitedcell-permeability. Among the proteins, aMTD/SD-fused SOCS3 recombinantprotein (HM₁₆₅S3B) showed the highest cell-permeability.

Example 7-2. Tissue-Permeability Test

To further investigate in vivo delivery of SOCS3 recombinant proteins,ICR mice (Doo-Yeol Biotech Co. Ltd., Seoul, Korea) were intraperitoneal(IP) injected with 600 μg/head of 10 μM FITC (Fluoresceinisothiocyanate, SIGMA, USA)-labeled SOCS3 proteins and sacrificed after2 hrs. From the mice, the liver, kidney, spleen, lung, heart, and brainwere removed and washed with PBS, and then placed on a dry ice, andembedded with an O.C.T. compound (Sakura). After cryosectioning at 20μm, tissue distributions of fluorescence-labeled-SOCS3 proteins indifferent organs was analyzed by fluorescence microscopy (Carl Zeiss,Gottingen, Germany)(FIG. 22).

As shown in FIG. 22, SOCS3 recombinant proteins fused to aMTD₁₆₅(HM₁₆₅S3, HM₁₆₅S3A and HM₁₆₅S3B) were distributed to a variety oftissues (liver, kidney, spleen, lung, heart and, to a lesser extent,brain). Liver showed highest levels of fluorescent cell-permeable SOCS3since intraperitoneal administration favors the delivery of proteins tothis organ via the portal circulation. SOCS3 containing aMTD₁₆₅ wasdetectable to a lesser degree in lung, spleen and heart. aMTD/SDB-fusedSOCS3 recombinant protein (HM₁₆₅S3B) showed the highest systemicdelivery of SOCS3 protein to the tissues compared to the SOCS3containing only aMTD (HM₁₆₅S3) or aMTD/SDA (HM₁₆₅S3A) proteins. Thesedata suggest that SOCS3 protein containing both of aMTD₁₆₅ and SDB leadsto higher cell-/tissue-permeability due to the increase in solubilityand stability of the protein, and it displayed a dramatic synergiceffect on cell-/tissue-permeability.

Example 7-2-2. Comparison Between aMTD/SD-Fused SOCS3 RecombinantProtein and Basic CPP/SD-Fused SOCS3 Recombinant Protein

The tissue-permeability of basic CPP/SD-fused SOCS3 recombinant proteinswas assessed by the same method as used in Example 7-2 except for aknown basic CPP (TAT or PolyR) being used instead of aMTD. The resultsof the assessment were shown in FIG. 100.

According to the results, only aMTD/SD-fused SOCS3 recombinant protein(HM₁₆₅S3B) exhibited superior cell-permeability.

Example 8-1 Biological Activity Test of iCP-SOCS3—Inhibition Activity ofIFN-γ-Induced STAT Phosphorylation

It was examined whether the iCP-SOCS3 recombinant proteins prepared byfusion with combinations of aMTD and SD inhibits activation of theJAK/STAT-signaling pathway.

PANC-1 Cells (KCLB, Seoul, South Korea) were treated with 10 ng/ml IFN-γ(R&D systems, Abingdon, UK) for 15 min and treated with either DMEM(vehicle) or 10 μM aMTD/SD-fused SOCS3 recombinant proteins for 2 hrs.The cells were lysed in RIPA lysis buffer (Biosesang, Seongnam, Korea)containing proteinase inhibitor cocktail (Roche, Indianapolis, Ind.,USA), incubated for 15 min at 4° C., and centrifuged at 13,000 rpm for10 min at 4° C. Equal amounts of lysates were separated on 10% SDS-PAGEgels and transferred to a nitrocellulose membrane. The membranes wereblocked using 5% skim milk in TBST and for western blot analysisincubated with the following antibodies: anti-phospho-STAT1 (CellSignaling Technology, USA) and anti-phospho-STAT3 (Cell SignalingTechnology, USA), then HRP conjugated anti-rabbit secondary antibody(Santacruz).

The well-known cytokine signaling inhibitory actions of SOCS3 areinflammation inhibition through i) inhibition of IFN-γ-mediated JAK/STATand ii) inhibition of LPS-mediated cytokine secretion. The ultimate testof cell-penetrating efficiency is a determination of intracellularactivity of SOCS3 proteins transported by aMTD. Since endogenous SOCS3are known to block phosphorylation of STAT1 and STAT3 by IFN-γ-mediatedJanus kinases (JAK) 1 and 2 activation, we demonstrated whethercell-permeable SOCS3 inhibits the phosphorylation of STATs. As shown inFIG. 23, All SOCS3 recombinant proteins containing aMTD (HM₁₆₅S3,HM₁₆₅S3A and HM₁₆₅S3B), suppressed IFN-γ-induced phosphorylation ofSTAT1 and STAT3. In contrast, STAT phosphorylation was readily detectedin cells exposed to HS3, which lacks the aMTD motif required formembrane penetration, indicating that HS3, which lacks an MTD sequencedid not enter the cells, has no biological activity.

Example 8-2. Biological Activity Test of iCP-SOCS3

Peritoneal macrophages were obtained from C3H/HeJ mice (Doo-Yeol BiotechCo. Ltd. Korea) Peritoneal macrophages were incubated with 10 μM SOCS3recombinant proteins (1:HS3, 2:HM₁₆₅S3, 3:HM₁₆₅S3A and 4:HM₁₆₅S3B,respectively) for 1 hr at 37° C. and then stimulated them with LPS(Lipopolysaccharide)(500 ng/ml) and/or IFN-γ (100 U/ml) without removingiCP-SOCS3 proteins for 3, 6, or 9 hrs. The culture media were collected,and the extracellular levels of cytokine (TNF-α, IL-6) were measured bya cytometric bead array (BD Pharmingen, San Diego, Calif., USA)according to the manufacturer's instructions.

The effect of cell-permeable SOCS3 proteins on cytokines secretion wasinvestigated. Treatment of C3H/HeJ primary peritoneal macrophages withSOCS3 proteins containing aMTD₁₆₅ suppressed TNF-α and IL-6 secretioninduced by the combination of IFN-γ and LPS by 50-90% during subsequent9 hrs of incubation (FIG. 24). In particular, aMTD₁₆₅/SDB-fused SOCS3recombinant protein showed the greatest inhibitory effect on cytokinesecretion. In contrast, cytokine secretion in macrophages treated withnon-permeable SOCS3 protein (HS3) was unchanged, indicating thatrecombinant SOCS3 lacking the aMTD doesn't affect intracellularsignaling. Therefore, we conclude that differences in the biologicalactivities of HM₁₆₅S3B as compared to HS3B are due to the differences inprotein uptake mediated by the aMTD sequence. In light ofsolubility/yield, cell-/tissue-permeability, and biological effect,SOCS3 recombinant protein containing aMTD and SDB (HM₁₆₅S3B) is aprototype of a new generation of improved cell-permeable SOCS3(iCP-SOCS3), and will be selected for further evaluation as a potentialanti-tumor agent.

Example 9. Preparation of Control Protein (Non-CP-SOCS3: HS3BRecombinant Protein)

As an experimental negative control, a SOCS3 recombinant protein havingno cell permeability was prepared.

According to Example 6-2, SOCS3 recombinant proteins lacking SD(HM₁₆₅S3) or both aMTD and SD (HS3) were found to be less soluble,produced lower yields, and showed tendency to precipitate when they wereexpressed and purified in E. coli. Therefore, we additionally designedand constructed SOCS3 recombinant protein containing only SDB (withoutaMTD₁₆₅: HS3B) as a negative control (FIG. 25). Preparation, expressionand purification, and measurement of solubility/yield of the recombinantproteins were performed in the same manner as in Examples 6-2 and 6-3.

TABLE 37 Recombinant Cargo SD Protein 5′ Primers 3′ Primers SOCS3 B HS3B5′-GGAATTCCATATGGTC 5′-CGCGTCGACTTAAA ACCCACAGCAAGTTTCC GGGTTTCCGAAGGCTTCGCCGCC-3′ GGCTATCTT-3′ (SEQ ID NO: 969) (SEQ ID NO: 970)

As expected, its solubility and yield increased compared to that ofSOCS3 proteins lacking SDB (HS3; FIG. 26). Therefore, HS3B proteins wereused as a control protein.

Example 10. Selection of aMTD for Cell-Permeability

After a basic structure of the stable recombinant proteins fused withcombinations of aMTD and SD was determined, 22 aMTDs were selected fordevelopment of iCP-SOCS3 recombinant protein (Tables 38 and 39), basedon the critical Factors, in order to examine which aMTD provides thehighest cell-/tissue-permeability.

For comparison, 5 kinds of random peptides that do not satisfying one ormore critical factors were selected (Table 40).

Solubility/yield and cell-permeability of 22 kinds of aMTD/SDB-fusedSOCS3 recombinant proteins, prepared by using primers of Table 41 in thesame manner as in Example 6-2, were analyzed according to Examples 6-3and 7-1, respectively.

TABLE 41 aMTD Amino Acid Cargo ID Sequence 5′ Primers 3′ Primers SOCS3MTM AAVLLPVLLAAP GGAATTCCATATGGCGGCGGTGCTGCTGCCGGTG CGCGTCGACTTAAAGGG(SEQ ID NO: 865) CTGCTGGCGGCGCCGGTCACCCACAGCAAGTTTCTTTCCGAAGGCTTGGCTATCCTT CCGCCGCC (SEQ ID NO: 857) (SEQ ID NO: 995)  44ALAVPVALLVAP GGAATTCCATATGGCGCTGGCGGTGCCGGTGGCGC (SEQ ID NO: 16)TGCTGGTGGCGCCGGTCACCCACAGCAAGTTTCCC GCCGCC (SEQ ID NO: 858)  81AALLPALAALLP GGAATTCCATATGGCGGCGCTGCTGCCGGCGCTGG (SEQ ID NO: 22)CGGCGCTGCTGCCGGTCACCCACAGCAAGTTTCCC GCCGCC (SEQ ID NO: 861) 123AAIIVPAALLAP GGAATTCCATATGGCGGCGATTATTGTGCCGGCGG (SEQ ID NO: 33)CGCTGCTGGCGCCGGTCACCCACAGCAAGTTTCCC GCCGCC (SEQ ID NO: 862) 162AVVALPAALIVP GGAATTCCATATGGCGGTGGTGGCGCTGCCGGCGG (SEQ ID NO: 40)CGCTGATTGTGCCGGTCACCCACAGCAAGTTTCCCG CCGCC (SEQ ID NO: 863) 281ALIVLPAAVAVP GGAATTCCATATGGCGCTGATTGTGCTGCCGGCGG (SEQ ID NO: 65)CGGTGGCGGTGCCGGTCACCCACAGCAAGTTTCCC GCCGCC (SEQ ID NO: 864) 324IVAVALPAALVP GGAATTCCATATGATTGTGGCGGTGGCGCTGCCGG (SEQ ID NO: 77)CGGCGCTGGTGCCGGTCACCCACAGCAAGTTTCCC GCCGCC (SEQ ID NO: 977) 364LVAAVAPALIVP GGAATTCCATATGCTGGTGGCGGCGGTGGCGCCGG (SEQ ID NO: 85)CGCTGATTGTGCCGGTCACCCACAGCAAGTTTCCCG CCGCC (SEQ ID NO: 978) 365AVIVVAPALLAP GGAATTCCATATGGCGGTGATTGTGGTGGCGCCGG (SEQ ID NO: 86)CGCTGCTGGCGCCGGTCACCCACAGCAAGTTTCCC GCCGCC (SEQ ID NO: 979) 622ALIVLAAPVAVP GGAATTCCATATGGCGCTGATTGTGCTGGCGGCGC (SEQ ID NO: 142)CGGTGGCGGTGCCGGTCACCCACAGCAAGTTTCCC GCCGCC (SEQ ID NO: 980) 662ALAVILAPVAVP GGAATTCCATATGGCGCTGGCGGTGATTCTGGCGC (SEQ ID NO: 822)CGGTGGCGGTGCCGGTCACCCACAGCAAGTTTCCC GCCGCC (SEQ ID NO: 981) 563ALAVIVVPALAP GGAATTCCATATGGCGCTGGCGGTGATTGTGGTGC (SEQ ID NO: 131)CGGCGCTGGCGCCGGTCACCCACAGCAAGTTTCCC GCCGCC (SEQ ID NO: 982) 899AVVIALPAVVAP GGAATTCCATATGGCGGTGGTGATTGCGCTGCCGG (SEQ ID NO: 229)CGGTGGTGGCGCCGGTCACCCACAGCAAGTTTCCC GCCGCC (SEQ ID NO: 983) 897AVIVPVAIIAAP GGAATTCCATATGGCGGTGATTGTGCCGGTGGCGAT (SEQ ID NO: 228)TATTGCGGCGCCGGTCACCCACAGCAAGTTTCCCG CCGCC (SEQ ID NO: 984) 623VAAAIALPAIVP GGAATTCCATATGGTGGCGGCGGCGATTGCGCTGC (SEQ ID NO: 143)CGGCGATTGTGCCGGTCACCCACAGCAAGTTTCCC GCCGCC (SEQ ID NO: 985) 908VALALAPVVVAP GGAATTCCATATGGTGGCGCTGGCGCTGGCGCCGG (SEQ ID NO: 237)TGGTGGTGGCGCCGGTCACCCACAGCAAGTTTCCC GCCGCC (SEQ ID NO: 986) 911VALALPAVVVAP GGAATTCCATATGGTGGCGCTGGCGCTGCCGGCGG (SEQ ID NO: 239)TGGTGGTGGCGCCGGTCACCCACAGCAAGTTTCCC GCCGCC (SEQ ID NO: 987)   2AAAVPLLAVVVP GGAATTCCATATGGCGGCGGCGGTGCCGCTGCTGG (SEQ ID NO: 2)CGGTGGTGGTGCCGGTCACCCACAGCAAGTTTCCC GCCGCC (SEQ ID NO: 988) 904AVLAVVAPVVAP GGAATTCCATATGGCGGTGCTGGCGGTGGTGGCGC (SEQ ID NO: 233)CGGTGGTGGCGCCGGTCACCCACAGCAAGTTTCCC GCCGCC (SEQ ID NO: 989) 481AIAIAIVPVALP GGAATTCCATATGGCGATTGCGATTGCGATTGTGCC (SEQ ID NO: 110)GGTGGCGCTGCCGGTCACCCACAGCAAGTTTCCCG CCGCC (SEQ ID NO: 990) 787AVALVPVIVAAP GGAATTCCATATGGCGGTGGCGCTGGTGCCGGTGA (SEQ ID NO: 177)TTGTGGCGGCGCCGGTCACCCACAGCAAGTTTCCC GCCGCC (SEQ ID NO: 991) 264LAAAPVVIVIAP GGAATTCCATATGCTGGCGGCGGCGCCGGTGGTGA (SEQ ID NO: 63)TTGTGATTGCGCCGGTCACCCACAGCAAGTTTCCCG CCGCC (SEQ ID NO: 992) 363AVLAVAPALIVP GGAATTCCATATGGCGGTGCTGGCGGTGGCGCCGG (SEQ ID NO: 84)CGCTGATTGTGCCGGTCACCCACAGCAAGTTTCCCG CCGCC (SEQ ID NO: 993) 121AIVALPALALAP GGAATTCCATATGGCGATTGTGGCGCTGCCGGCGC (SEQ ID NO: 32)TGGCGCTGGCGCCGGTCACCCACAGCAAGTTTCCC GCCGCC (SEQ ID NO: 994) rPEPTIDEAmino Acid Cargo ID Sequence 5′ Primers 3′ Primers SOCS3 921IEWFVVLPLVVP GGAATTCCATATGATTTGGTGGTTTGTGGTGCT CGCGTCGACTTAAAGGGTTTCCG(SEQ ID NO: 931) GCCGCTGGTGGTGCCGGTCACCCACAGCAAG AAGGCTTGGCTATCTTTTTCCCGCCGCC (SEQ ID NO: 996) (SEQ ID NO: 1001)  16 NNSCTTYTNGSQGGAATTCCATATGAACAACAGCTGCACCACCTA (SEQ ID NO: 890)TACCAACGGCAGCCAGGTCACCCACAGCAAGT TTCCCGCCGCC (SEQ ID NO: 997)  67LDAEVPLADDVP GGAATTCCATATGCTGGATGCGGAAGTGCCGC (SEQ ID NO: 906)TGGCGGATGATGTGCCGGTCACCCACAGCAAG TTTCCCGCCGCC (SEQ ID NO: 998)  29VLPPLPVLPVLP GGAATTCCATATGGTGCTGCCGCCGCTGCCGG (SEQ ID NO: 924)TGCTGCCGGTGCTGCCGGTCACCCACAGCAA GTTTCCCGCCGCC (SEQ ID NO: 999) 700GTSNTCQSNQNS GGAATTCCATATGGGCACCAGCAACACCTGCC (SEQ ID NO: 951)AGAGCAACCAGAACAGCGTCACCCACAGCAAG TTTCCCGCCGCC (SEQ ID NO: 1000)

As shown in FIGS. 27 to 34, it was confirmed that most of theaMTD/SDB-fused SOCS3 recombinant proteins showed high solubility andyield and high cell permeability by aMTD. However, Randompeptide-SOCS3-SDB recombinant protein showed remarkably low cellpermeability.

Example 11-1. Investigation of Biological Activity for Determination ofOptimal aMTD-Fused SOCS3 Recombinant Protein-1

Four kinds of aMTD/SD-fused SOCS3 recombinant proteins having high cellpermeability and one kind of aMTD/SD-fused SOCS3 recombinant proteinhaving the lowest cell permeability were selected, and their biologicalactivity was analyzed.

PANC-1 cells (pancreatic carcinoma cell line) were seeded in a 16-wellchamber slide at a density of 5×10³ cells/well, and then treated with 10uM of aMTD/SD-fused SOCS3 for 24 hours. Apoptotic cells were analyzedusing terminal dUTP nick-end labeling (TUNEL) assay with In Situ CellDeath Detection kit TMR red (Roche, 4056 Basel, Switzerland). Cells weretreated with either 10 μM SOCS3 recombinant protein or buffer alone for16 hrs with 2% fetal bovine serum (Hyclone, Logan, Utah, USA). Treatedcells were washed with cold PBS two times, fixed in 4% paraformaldehyde(PFA, Junsei, Tokyo, Japan) for 1 hr at room temperature, and incubatedin 0.1% Triton X-100 for 2 min on the ice. Cells were washed with coldPBS twice, and treated TUNEL reaction mixture for 1 hr at 37° C. indark, washed cold PBS three times and observed by fluorescencemicroscopy (Nikon, Tokyo, Japan).

As shown in FIG. 35, most of the aMTD/SDB-fused SOCS3 recombinantproteins induced cell death of pancreatic carcinoma cells, and of them,aMTD₁₆₅ or aMTD₃₂₄-fused SOCS3 recombinant protein induced death of thelargest number of cancer cells.

Example 11-2. Investigation of Biological Activity for Determination ofOptimal aMTD-Fused SOCS3 Recombinant Protein-2

AGS cells (gastric carcinoma cell line) (American Type CultureCollection; ATCC) were seeded in a 12-well plate at a density of 1×10⁵cells/well, and then treated with 10 uM of aMTD/SD-fused SOCS3 for 14hours. Cancer cell death was analyzed by Annexin V analysis. AnnexinV/7-Aminoactinomycin D (7-AAD) staining was performed using flowcytometry according to the manufacturer's guidelines (BD Pharmingen, SanDiego, Calif., USA). Briefly, cells were washed three times withice-cold PBS. The cells were then resuspended in 100 μl of bindingbuffer and incubated with 1 μl of 7-AAD and 1 μl of annexin V-PE for 30min in the dark at 37° C. Flow cytometric analysis was immediatelyperformed using a guava easyCyte™ 8 Instrument (Merck Millipore,Darmstadt, Germany).

As shown in FIG. 36, most of the aMTD/SDB-fused SOCS3 recombinantproteins induced cell death of gastric carcinoma cells, and of them,aMTD₁₆₅ or aMTD₂₈₁-fused SOCS3 recombinant protein induced death of thelargest number of cancer cells.

Example 11-3. Investigation of Biological Activity for Determination ofOptimal aMTD-Fused SOCS3 Recombinant Protein-3

AGS cells (gastric cancer cell line) were seeded in a 12 well plate at adensity of 2.5×10⁵ per well, grown to 90% confluence. Confluent AGScells were incubated with 10 μM HM#S3B in serum-free medium for 2 hrsprior to changing the growth medium (DMEM/F12, Hyclone, Logan, Utah,USA) and washed twice with PBS, and the monolayer at the center of thewell was “wounded” by scraping with a sterilized white pipette tip.Cells were cultured for an additional 24 hrs and cell migration wasobserved by phase contrast microscopy (Nikon, ECLIPSE Ts2). Themigration was quantified by counting the number of cells that migratedfrom the wound edge into the clear area.

As shown in FIG. 37, most of the aMTD/SDB-fused SOCS3 recombinantproteins inhibited cell migration of gastric carcinoma cells, and ofthem, aMTD₁₆₅ or aMTD₉₀₄-fused SOCS3 recombinant protein showed the mosteffective inhibition of cancer cell migration.

Solubility/yield, permeability, and biological activity of 22 kinds ofthe aMTD-fused recombinant proteins were examined in Examples 10 to11-3, and as a result, the aMTD₁₆₅/SDB-fused SOCS3 recombinant proteinwas found to show the most excellent effect (FIG. 38). Therefore, theaMTD₁₆₅-fused recombinant protein was used in the subsequent experiment.

Example 12-1. Preparation of iCP-SOCS3 Recombinant Protein andInvestigation of Equivalence Thereof-1

To develop a new drug as an anticancer agent, His-tag-removed iCP-SOCS3recombinant protein was prepared and equivalence of His-Tag+, -iCP-SOCS3was investigated.

Histidine-tag free human SOCS3 proteins were constructed by amplifyingthe SOCS3 cDNA (225 amino acids) for aMTD fused to SOCS3 cargo. The PCRreactions (100 ng genomic DNA, 10 pmol each primer, each 0.2 mM dNTPmixture, 1× reaction buffer and 2.5 U Pfu(+) DNA polymerase (Doctorprotein, Korea)) were digested on the restriction enzyme site betweenNde I (5′) and Sal I (3′) involving 35 cycles of denaturing (95° C.),annealing (62° C.), and extending (72° C.) for 45 sec each. For the lastextension cycle, the PCR reactions remained for 10 min at 72° C. The PCRproducts were subcloned into pET-26b(+) (Novagen, Darmstadt, Germany).Coding sequence for SDB fused to C terminus of aMTD-SOCS3 was cloned atBamHI (5′) and Sal1 (3′) in pET-26b(+) from PCR-amplified DNA segmentsand confirmed by DNA sequence analysis of the resulting plasmids.

TABLE 42 Recombinant Cargo SD Protein 5′ Primers 3′ Primers SOCS3 — HS35′-GGAATTCCATATGGTCA 5′-CCCGGATCCTTAAAGC CCCACAGCAAGTTTCCCGCGGGGCATCGTACTGGTCC CGCC-3′ AGGAA-3′ (SEQ ID NO: 955) (SEQ ID NO: 956)HM₁₆₅S3 5′-GGAATTCCATATGGCGCT 5′-CCCGGATCCTTAAAG GGCGGTGCCGGTGGCGCTCGGGGCATCGTACTGGT GGCGATTGTGCCGGTCACC CCAGGAA-3′ CACAGCAAGTTTC-3′(SEQ ID NO: 958) (SEQ ID NO: 957) A HM₁₆₅S3A 5′-GGAATTCCATATGGCGC5′-CGCGTCGACTTACCTCG TGGCGGTGCCGGTGGCGC GCTGCACCGG CACGGAGTGGCGATTGTGCCGGTCAC ATGAC-3′ CCACAGCAAGTTTC-3′ (SEQ ID NO: 960)(SEQ ID NO: 959) B HM₁₆₅S3B 5′-GGAATTCCATATGGCGCT 5′-CGCGTCGACTTAAAGGGGCGGTGCCGGTGGCGCT GTTTCCGAAGGCTTGGCT GGCGATTGTGCCGGTCACC ATCTT-3′CACAGCAAGTTTC-3′ (SEQ ID NO: 962) (SEQ ID NO: 961) C HM₁₆₅S3C5′-GGAATTCCATATGGCGC 5′-GCGTCGACTTAGGC TGGCGGTGCCGGTGGCGCTCAGGTTAGCGTCGAG-3′ GGCGATTGTGCCGGTCACC (SEQ ID NO: 964) CACAGCAAGTTTC-3′(SEQ ID NO: 963) D HM₁₆₅S3D 5′-GGAATTCCATATGGCGC 5′-GCGTCGACTTATTTTTGGCGGTGCCGGTGGCGC TTCTCGGACAGATA-3′ TGGCGATTGTGCCGGTCAC(SEQ ID NO: 966) CCACAGCAAGTTTC-3′ (SEQ ID NO: 965) E HM₁₆₅S3E5′-GGAATTCCATATGGCGC 5′-ACGCGTCGACTTAA TGGCGGTGCCGGTGGCGCCCTCCAATCTGTTCGCG TGGCGATTGTGCCGGTCA GTGAGCCTC-3′ CCCACAGCAAGTTTC(SEQ ID NO: 968) (SEQ ID NO: 967) Recombinant Cargo SD Protein 5′Primers 3′ Primers SOCS3 B HM₁₆₅S3B 5′-GGAATTCCATATGGCGCTGGC5′-CGCGTCGACTTAAAGGGTTT GGTGCCGGTGGCGCTGGCGATT CCGAAGGCTTGGCTATCTT-3′GTGCCGGTCACCCACAGCAAGT (SEQ ID NO: 860) TTC-3′ (SEQ ID NO: 859)

Expression, purification and solubility/yield were measured in the samemanner as in Example 6-2 to 6-3, and as a result, his-tag-removedM₁₆₅S3B was found to have high solubility/yield (FIG. 39).

Example 12-2. Preparation of iCP-SOCS3 Recombinant Protein andInvestigation of Equivalence Thereof-2

In the same manner as in Example 7-1, RAW264.7 cells were treated withFITC-labeled HS3B, HM₁₆₅S3B, and M₁₆₅S3B proteins, and cell permeabilitywas evaluated.

As shown in FIG. 40, both HM₁₆₅S3B and M₁₆₅S3B were found to have highcell permeability.

Example 12-3. Preparation of iCP-SOCS3 Recombinant Protein andInvestigation of Equivalence Thereof-3

To investigate biological activity equivalence of the HM₁₆₅S3B andM₁₆₅S3B recombinant proteins, induction of apoptosis of gastriccarcinoma cell line (AGS) was analyzed by Annexin V staining in the samemanner as in Example 11-2, and inhibition of migration was analyzed inthe same manner as in Example 11-3.

As shown in FIG. 41, it was confirmed that both HM₁₆₅S3B and M₁₆₅S3Bshowed high anticancer efficacy and M₁₆₅S3B exhibited efficacyequivalent to or higher than HM₁₆₅S3B.

Example 12-4. Preparation of iCP-SOCS3 Recombinant Protein andInvestigation of Equivalence Thereof-4

In silico MHC class II binding analysis using iTope™ (ANTITOPE.LTD)revealed changing the V28 p1 anchor residue in SDB sequence to L makesthis region human germline and as such both MHC class II bindingpeptides within this region would be expected to be low risk due to Tcell tolerance.

To prepare humanized SDB domain, iCP-SOCS3 was prepared in the samemanner as in Example 6-1, except that SDB′ having a substitution ofvaline with leucine at an amino acid position 28 was used (FIG. 94).Further, protein purification was performed in the same manner as inExample 6-2 using the primer as below (Table 43).

TABLE 43 Recombinant Cargo SD Protein 5′ Primers 3′ Primers SOCS3 — HS35′-GGAATTCCATATGGTCA 5′-CCCGGATCCTTAAAGC CCCACAGCAAGTTTCCCGCGGGGCATCGTACTGGTCC CGCC-3′ AGGAA-3′ (SEQ ID NO: 955) (SEQ ID NO: 956)HM₁₆₅S3 5′-GGAATTCCATATGGCGCT 5′-CCCGGATCCTTAAAG GGCGGTGCCGGTGGCGCTCGGGGCATCGTACTGGT GGCGATTGTGCCGGTCACC CCAGGAA-3′ CACAGCAAGTTTC-3′(SEQ ID NO: 958) (SEQ ID NO: 957) A HM₁₆₅S3A 5′-GGAATTCCATATGGCGC5′-CGCGTCGACTTACCTCG TGGCGGTGCCGGTGGCGC GCTGCACCGG CACGGAGTGGCGATTGTGCCGGTCAC ATGAC-3′ CCACAGCAAGTTTC-3′ (SEQ ID NO: 960)(SEQ ID NO: 959) B HM₁₆₅S3B 5′-GGAATTCCATATGGCGCT 5′-CGCGTCGACTTAAAGGGGCGGTGCCGGTGGCGCT GTTTCCGAAGGCTTGGCT GGCGATTGTGCCGGTCACC ATCTT-3′CACAGCAAGTTTC-3′ (SEQ ID NO: 962) (SEQ ID NO: 961) C HM₁₆₅S3C5′-GGAATTCCATATGGCGC 5′-GCGTCGACTTAGGC TGGCGGTGCCGGTGGCGCTCAGGTTAGCGTCGAG-3′ GGCGATTGTGCCGGTCACC (SEQ ID NO: 964) CACAGCAAGTTTC-3′(SEQ ID NO: 963) D HM₁₆₅S3D 5′-GGAATTCCATATGGCGC 5′-GCGTCGACTTATTTTTGGCGGTGCCGGTGGCGC TTCTCGGACAGATA-3′ TGGCGATTGTGCCGGTCAC(SEQ ID NO: 966) CCACAGCAAGTTTC-3′ (SEQ ID NO: 965) E HM₁₆₅S3E5′-GGAATTCCATATGGCGC 5′-ACGCGTCGACTTAA TGGCGGTGCCGGTGGCGCCCTCCAATCTGTTCGCG TGGCGATTGTGCCGGTCA GTGAGCCTC-3′ CCCACAGCAAGTTTC(SEQ ID NO: 968) (SEQ ID NO: 967) Recombinant Cargo SD Protein 5′Primers 3′ Primers SOCS3 B* HM₁₆₅S3B 5′-GGAATTCCATATGGCGCTGG5′-CGCGTCGACTTAAAGG CGGTGCCGGTGGCGCTGGCGA GTTTCCGAAGGCTTGGCTTTGTGCCGGTCACCCACAGCA ATCTT-3′ AGTTTC-3′ (SEQ ID NO:  972)(SEQ ID NO: 971)

As shown in FIG. 44, both HM₁₆₅S3B and HM₁₆₅S3B′(V28L) were found tohave high solubility/yield.

Example 12-5. Preparation of iCP-SOCS3 Recombinant Protein andInvestigation of Equivalence Thereof-5

In the same manner as in Example 7-1, RAW264.7 cells were treated withFITC-labeled HM₁₆₅S3B and HM₁₆₅S3B′(V28L) proteins, and cellpermeability was evaluated.

As shown in FIG. 45, both HM₁₆₅S3B and HM₁₆₅S3B′(V28L) were found tohave high cell permeability.

Example 12-6. Preparation of iCP-SOCS3 Recombinant Protein andInvestigation of Equivalence Thereof-6

To investigate biological activity equivalence of the HM₁₆₅S3B andHM₁₆₅S3B′(V28L) recombinant proteins, anti-proliferative activity wasexamined and induction of apoptosis of gastric carcinoma cell line (AGS)was analyzed by Annexin V staining in the same manner as in Example11-2, and inhibition of migration was analyzed in the same manner as inExample 11-3.

Antiproliferative activity were evaluated with the CellTiter-Glo CellViability Assay. AGS cells (3×10³/well) were seeded in 96 well plates.The next day, cells were treated with DMEM (vehicle) or 10 μM HM₁₆₅S3B,HM₁₆₅S3B′(V28L) for 96 hrs in the presence of serum (2%). Proteins werereplaced daily. Cell growth and survival were evaluated with theCellTiter-Glo Cell Viability Assay (Promega, Madison, Wis.).Measurements using a Luminometer (Turner Designs, Sunnyvale, Calif.)were conducted following the manufacturer's protocol.

It was confirmed that both HM₁₆₅S3B and HM₁₆₅S3B′(V28L) showed highanti-proliferative effects on gastric carcinoma cells (FIG. 46), andalso effects of inducing apoptosis (FIG. 47) and of inhibiting migrationof gastric carcinoma cells (FIG. 48), and in particular, HM₁₆₅S3B′(V28L)exhibited efficacy equivalent to or higher than HM₁₆₅S3B.

Example 12-7. Preparation of iCP-SOCS3 Recombinant Protein andInvestigation of Equivalence Thereof-7

iCP-SOCS3 of BS3M₁₆₅ structure was prepared in the same manner as inExample 6-1, and B′S3M₁₆₅ iCP-SOCS3 was also prepared by humanized SDBdomain (FIG. 49a ).

TABLE 44 Recombinant Cargo SD Protein 5′ Primers 3′ Primers SOCS3 BBS3M₁₆₅ 5′-GGAATTCCATATG 5′-ACGCGTCGACTTAC ATGGCAGAACAAAGCGAC-3′GCCAGCGCCACCG (SEQ ID NO: 973) GCACCGCCAGCGC AATCACCGGAAGCGGGGCATCGTACTGG TCCAG-3′ (SEQ ID NO: 974) B* B*S3M₁₆₅ 5′-GGAATTCCATATG5′-ACGCGTCGACTTAC ATGGCAGAACAAAGCGAC-3′ GCCAGCGCCACCG (SEQ ID NO: 975)GCACCGCCAGCGC AATCACCGGAAGCG GGGCATCGTACTGG TCCAG-3′ (SEQ ID NO: 976)

Expressions and purifications of iCP-SOCS3 recombinant protein (BS3M₁₆₅,B′S3M₁₆₅) in E. coli (bottom) were analyzed in the same manner as inExamples 6-2 and 6-3, respectively, and shown in FIG. 49b . Further, E.coli codon-optimized iCP-SOCS3 was prepared.

Example 13. Test of Biological Activity of iCP-SOCS3—Inhibition Activityof IFN-γ-Induced STAT Phosphorylation

Whether iCP-SOCS3 (HM₁₆₅S3B) recombinant protein inhibits activation ofthe JAK/STAT-signaling pathway was examined by the method of Example8-1.

PANC-1 Cells (KCLB, Seoul, South Korea) were treated with 10 ng/ml IFN-γ(R&D systems, Abingdon, UK) for 15 min and treated with either DMEM(vehicle) or 1, 5, 10 μM aMTD/SD-fused SOCS3 recombinant proteins for 2hrs. The cells were lysed in RIPA lysis buffer (Biosesang, Seongnam,Korea) containing proteinase inhibitor cocktail (Roche, Indianapolis,Ind., USA), incubated for 15 min at 4° C., and centrifuged at 13,000 rpmfor 10 min at 4° C. Equal amounts of lysates were separated on 10%SDS-PAGE gels and transferred to a nitrocellulose membrane. Themembranes were blocked using 5% skim milk in TBST and for western blotanalysis incubated with the following antibodies: anti-phospho-STAT3(Cell Signaling Technology, USA), then HRP conjugated anti-rabbitsecondary antibody (Santacruz).

The well-known cytokine signaling inhibitory actions of SOCS3 areinflammation inhibition through i) inhibition of IFN-γ-mediated JAK/STATand ii) inhibition of LPS-mediated cytokine secretion. The ultimate testof cell-penetrating efficiency is a determination of intracellularactivity of SOCS3 proteins transported by aMTD. Since endogenous SOCS3are known to block phosphorylation of STAT3 by IFN-γ-mediated Januskinases (JAK) 1 and 2 activation, we demonstrated whether cell-permeableSOCS3 inhibits the phosphorylation of STATs. As shown in FIG. 50,iCP-SOCS3 (HM₁₆₅S3B) suppressed IFN-γ-induced phosphorylation of STAT3in dose dependent manner. In contrast, STAT phosphorylation was readilydetected in cells exposed to HS3B, which lacks the aMTD motif requiredfor membrane penetration, indicating that HS3B, which lacks an MTDsequence did not enter the cells, has no biological activity.

Example 14. Investigation of aMTD-Mediated Intracellular DeliveryMechanism

The mechanism of aMTD₁₆₅-mediated intracellular delivery wasinvestigated.

(1) RAW 264.7 cells were pretreated with 100 mM EDTA for 3 hours, andthen treated with 10 μM of iCP-SOCS3 (HM₁₆₅S3B) recombinant protein for1 hour, followed by flow cytometry in the same manner as in Example 7-1(FIG. 51A).

(2) RAW 264.7 cells were pretreated with 5 μg/ml of proteinase K for 10minutes, and then treated with 10 μM of iCP-SOCS3 (HM₁₆₅S3B) recombinantprotein for 1 hour, followed by flow cytometry (FIG. 51B).

(3) RAW 264.7 cells were pretreated with 20 μM taxol for 30 minutes, andthen treated with 10 μM of iCP-SOCS3 (HM₁₆₅S3B) recombinant protein for1 hour, followed by flow cytometry (FIG. 52A).

(4) RAW 264.7 cells were pretreated with 1 mM ATP and 10 μM antimycinsingly or in combination for 2 hours, and then treated with 10 μM ofiCP-SOCS3 (HM₁₆₅S3B) recombinant protein for 1 hour, followed by flowcytometry (FIG. 52B).

(5) RAW 264.7 cells were left at 4° C. and 37° C. for 1 hour,respectively, and then treated with 10 μM of iCP-SOCS3 (HM₁₆₅S3B)recombinant protein for 1 hour, followed by flow cytometry (FIG. 53).

The aMTD-mediated intracellular delivery of SOCS3 protein did notrequire protease-sensitive protein domains displayed on the cell surface(FIG. 51B), microtubule function (FIG. 52A), or ATP utilization (FIG.52B), since aMTD₁₆₅-dependent uptake, compare to HS3 and HS3B, wasessentially unaffected by treating cells with proteinase K, taxol, orthe ATP depleting agent, antimycin. Conversely, iCP-SOCS3 (HM₁₆₅S3B)proteins uptake was blocked by treatment with EDTA and low temperature(FIGS. 51A and 53), indicating the importance of membrane integrity andfluidity for aMTD-mediated protein transduction.

Moreover, whether cells treated with iCP-SOCS3 (HM₁₆₅S3B) protein couldtransfer the protein to neighboring cells were also tested.

For this, RAW 264.7 cells were treated with 10 μM of FITC-labelediCP-SOCS3 (HM₁₆₅S3B) recombinant protein for 1 hour. Thereafter, thesecells were co-cultured with PerCP-Cy5.5-CD14-stained RAW 264.7 cells for2 hours. Cell-to-cell protein transfer was assessed by flow cytometry,scoring for CD14/FITC double-positive cells. Efficient cell-to-celltransfer of HM₁₆₅S3B, but not HS3 or HS3B (FIG. 54), suggests that SOCS3recombinant proteins containing aMTD₁₆₅ are capable of bidirectionalpassage across the plasma membrane.

Example 14-2. Investigation of Basic CPP-Mediated Intracellular DeliveryMechanism

The mechanism of basic CPP (TAT and PolyR)-mediated intracellulardelivery was also investigated in the same manner as in Example 7-1 andExample 14.

As shown in FIG. 101, it was confirmed that aMTD165/SD-fused SOCS3recombinant proteins are independent to cell surface receptor (FIG.101A) and the cell-permeability of aMTD165/SD-fused SOCS3 recombinantproteins is not due to endocytosis (FIG. 101B).

Whether cells treated with aMTD165/SD-fused SOCS3, TAT/SD-fused SOCS3,and PolyR/SD-fused SOCS3 could transfer the protein to neighboring cellswere also tested on a molecular level in the same manner as in Example13.

For this, RAW 264.7 cells were treated with 5 μM of FITC-labeledHM₁₆₅S3B, HTS3B for 2 hour and washed with PBS two times. Thereafter,they were seeded on PANC-1 cell, incubated for 2 hours and treated with20 ng/ml of IFN-γ for 15 minutes, followed by Western blotting in thesame manner as in Example 8-1. And Cell-to-cell protein transfer wasassessed by flow cytometry.

As shown in FIG. 102, efficient cell-to-cell transfer of HM₁₆₅S3B, butnot HTS3B or HRS3B, suggests that only SOCS3 recombinant proteinscontaining aMTD165 are capable of bidirectional passage across theplasma membrane.

Moreover, as shown in FIG. 103, phospho-STAT3 was only reduced in cellstreated with HM₁₆₅S3B.

Example 15. Investigation of Bioavailability of iCP-SOCS3

To investigate BA of the iCP-SOCS3 (HM₁₆₅S3B) recombinant proteins, ICRmice (Doo-Yeol Biotech Co. Ltd., Seoul, Korea) were intravenous (IV)injected with 600 μg/head of 10 μM FITC (Fluorescein isothiocyanate,SIGMA, USA)-labeled SOCS3 recombinant proteins (HS3B, HM₁₆₅S3B) andafter 15 min, 30 min, 1H, 2H, 4H, 8H, 12H, 16H, 24H, 36H, 48H, mice ofeach group were sacrificed. From the mice, peripheral blood mononuclearcells (PBMCs), splenocytes, and hepatocytes were separated.

Further, the spleen was removed and washed with PBS, and then placed ona dry ice and embedded in an O.C.T. compound (Sakura). Aftercryosectioning at 20 μm, tissue distributions offluorescence-labeled-SOCS3 proteins in different organs was analyzed byfluorescence microscopy (Carl Zeiss, Gottingen, Germany).

Isolation of PBMC

After anesthesia with ether, ophthalmectomy was performed and the bloodwas collected therefrom using a 1 ml syringe. The collected blood wasimmediately put in an EDTA tube and mixed well. The blood wascentrifuged at 4,000 rpm and 4° C. for 5 minutes, and plasma wasdiscarded and only buffy coat was collected in a new microtube. 0.5 mlof RBC lysis buffer (Sigma) was added thereto, followed by vortexing.The microtube was left at room temperature for 5 minutes, and thencentrifuged at 4,000 rpm and 4° C. for 5 minutes. 0.3 ml of PBS wasadded to a pellet, followed by pipetting and flow cytometry (FlowJocytometric analysis software, Guava, Millipore, Darmstadt, Germany).

Isolation of Splenocytes and Hepatocytes

Mice were laparotomized and the spleen or liver were removed. The spleenor liver thus removed was separated into single cells using a CellStrainer (SPL, Korea). These cells were collected in a microtube,followed by centrifugation at 4,000 rpm and 4° C. for 5 minutes. 0.5 mlof RBC lysis buffer was added thereto, followed by vortexing. Themicrotube was left at room temperature for 5 minutes, and thencentrifuged at 4,000 rpm and 4° C. for 5 minutes. 0.5 ml of PBS wasadded to a pellet, followed by pipetting and flow cytometry (FlowJocytometric analysis software, Guava, Millipore, Darmstadt, Germany).

As shown in FIG. 55, in PBMCs, the maximum permeability of iCP-SOCS3 wasobserved at 30 minutes, and in splenocytes, the maximum permeability ofiCP-SOCS3 was observed at 2 hours and maintained up to 16 hours. Inhepatocytes, the maximum permeability of iCP-SOCS3 was observed at 15minutes and maintained up to 16 hours. As shown in FIG. 56, in thepancreas tissue, very high distribution of iCP-SOCS3 was observed at 2hours, and maintained up to 8 hours. Therefore, it can be seen thatiCP-SOCS3 is rapidly delivered from blood to various tissues within 2hours, and maintained up to 8-16 hours depending on the tissues.

Example 16. Investigation of Anticancer Efficacy of iCP-SOCS3Recombinant Protein

To develop the iCP-SOCS3 recombinant protein as a therapeutic agent forsolid tumors, its permeability to solid tumors (gastric cancer,colorectal cancer, breast cancer, glioblastoma cell line) and varioustissues was investigated.

AGS (gastric cancer cell line), HCT116 (colorectal cancer cell line),MDA-MB-231 (breast cancer cell line), and U-87 MG (glioblastoma cellline) cells (Korean cell line bank, Korea) were seeded in a 12 wellplate, grown to 90% confluence. Cells were treated with 10 μMFITC-labeled iCP-SOCS3 recombinant proteins and cultivated for lhr at37° C.

After cultivation, the cells were treated with proteinase K (10 μg/mL,SIGMA, USA) and washed three times with ice-cold PBS (Phosphate-bufferedsaline, Hyclone, USA) to remove surface-bound proteins, and internalizedproteins were measured by flow cytometry (FlowJo cytometric analysissoftware, Guava, Millipore, Darmstadt, Germany). Untreated cells (gray)and equimolar concentration of unconjugated FITC (FITC only,green)-treated cells were served as control (FIG. 57).

The permeability to various tissues was analyzed at 2 hours afterprotein injection by the method described in Example 7-3.

As shown in FIG. 57, iCP-SOCS3 recombinant proteins (FITC-HM₁₆₅S3SB)promoted the transduction into cultured various cancer cells. Incontrast, SOCS3 proteins containing non-aMTD (FITC-HS3 and FITC-HS3B)did not appear to enter cells.

In addition, iCP-SOCS3 recombinant proteins (FITC-HM₁₆₅S3SB) enhancedthe systemic delivery to various tissues after intraperitoneal injection(FIG. 58). Therefore, these data indicate that iCP-SOCS3 protein couldbe intracellularly delivered and distributed to the various cancer cellsand various tissues, contributing for beneficial biotherapeutic effects.

Example 17. Investigation of Anticancer Efficacy of iCP-SOCS3Recombinant Protein Example 17-1. Investigation of Anti-Cancer Efficacyof iCP-SOCS3 Recombinant Protein in Gastric Cancer Cells

To develop the iCP-SOCS3 recombinant protein as a mechanism-specifictherapeutic agent for solid tumors, SOCS3 levels endogenously expressedand activation of JAK/STAT-signaling pathway were investigated indifferent gastric cancer cell lines and normal cells, and normal hepaticcells.

Example 17-1-1. Analysis of Hypermethylation Level in Gastric CancerCells

SOCS3 expression is suppressed due to methylation in cancer cells, andtherefore, inflammation or cancer development is increased. Further, toinvestigate the effect of cell permeable SOCS3, a cancer cell line whereendogenous SOCS3 expression is suppressed should be selected or appliedto a model, and therefore, the present experiments were performed.

Genomic DNA was extracted from cancer cell line using an Exgene™ TissueSV mini kit (Geneall®, Korea). DNA was quantified, and experiments wereperformed using 500 ng of gDNA and an EZ DNA Methylation-Gold™ kit (ZYMOResearch, Orange, Calif., USA) according to the manufacturer'sinstructions. DNA was used to perform PCR, and methylation andunmethylation of endogenous SOCS3 were qualitatively analyzed byelectrophoresis. In this regard, the primers used are as follows.

Unmethyl-F was 5′-tag tgt gta agt tgt agg aga gtg g-3′(SEQ ID NO: 816),Unmethyl-R was 5′-cta aac ata aaa aaa taa cac taa tcc aaa-3′ (SEQ ID NO:817), Methyl-F was 5′-gta gtg cgt aag ttg tag gag agc-3′ (SEQ ID NO:818), Methyl-R was 5′-gta aaa aaa taa cgc taa tcc gaa-3′ (SEQ ID NO:819), PCR was performed for 30 cycles consisting of pre-denaturation at95° C. for 5 minutes, denaturation at 95° C. for 30 seconds, annealingat 60° C. for 45 seconds, extension at 72° C. for 1 minute, and finalextension at 72° C. for 8 minutes.

As shown in FIG. 95, unmethylation of SOCS3 was observed in HEK293 andHaCaT cells which are normal cells, whereas hypermethylation of thepromoter region of SOCS3 gene was observed in MNK75, MKN74, MKN45,MKN28, AGS, STKM2, NCI-N87 which are gastric cancer cell lines (U:unmethylated SOCS3, M: methylated SOCS3). These results indicate thatSOCS3 is silenced by hypermethylation in gastric cancer cell lines.

Example 17-1-2. Analysis of Expression Level of Endogenous SOCS3 mRNA inGastric Cell Line

SOCS3 mRNA expression levels in cancer cells were analyzed by RT-PCR.mRNAs were isolated from normal cell lines and cancer cell linesaccording to a method provided in a manufacturer's sheet of Hybrid-R(Geneall, Korea), and PCR was performed using SOCS3 primer F 5′-cct actgaa ccc tcc tcc ga-3′ (SEQ ID NO: 820) and SOCS3 primer R 5′-gca get gggtga ctt tct ca-3′ (SEQ ID NO: 821) for 30 cycles consisting ofdenaturing (95° C.), annealing (60° C.), and extending (72° C.) for 45seconds each.

It was confirmed that high expression levels of SOCS3 were observed inthe normal HEK293 whereas low expression levels of SOCS3 were observedin the gastric cancer cell lines, except MKN74 (FIG. 59).

Example 17-1-3. Analysis of Endogenous SOCS3 and JAK/STAT SignalingActivation Status in Gastric Cell Line

JAK/STAT3 activation in cancer cells was analyzed by Western blotanalysis. The normal HEK293 cells, and gastric cancer cell lines, MKN75,MKN74, MKN45, MKN28, AGS, STKM2, and NCI-N87 were washed with PBS, andthen the cells were lysed in RIPA lysis buffer (Biosesang, Seongnam,Korea) containing protease inhibitor cocktail (Roche, Indianapolis,Ind., USA), incubated for 15 min at 4° C., and centrifuged at 13,000 rpmfor 10 min at 4° C. to isolate proteins, followed by Western blotting inthe same manner as in Example 8-1.

As shown in FIG. 60, high expression levels of SOCS3 and low levels ofphosphorylations of p-JAK1, p-JAK2, p-STAT1, and p-STAT3 were observedin normal HEK293 cells. Low expression levels of SOCS3 gene wereobserved in the gastric cancer cell lines, compared to the normal cellline, and high levels of p-JAK1 and p-JAK2 in MKN75, high levels ofp-STAT1 in MKN75, MKN74, MKN28, and NCI-N87, and high levels of p-STAT3in MKN75, MKN45, AGS, STKN2, and NCI-N87. These results suggest apossibility of developing a mechanism-specific anticancer agent, becauseSOCS3-deficient cancer cells can be replenished with cell permeableproteins, and activated JAK/STAT-signaling can be negatively regulated.

Example 17-2. Investigation of Anti-Cancer Efficacy of iCP-SOCS3Recombinant Protein

To develop the iCP-SOCS3 recombinant protein as a mechanism-specifictherapeutic agent for solid tumors, SOCS3 levels endogenously expressedand activation of JAK/STAT-signaling pathway were investigated indifferent colorectal cell lines, normal cells, and normal hepatic cells.

Example 17-2-1. Analysis of Hypermethylation Level in Colorectal CancerCells

SOCS3 expression is suppressed due to methylation in cancer cells, andtherefore, inflammation or cancer development is increased. Further, toinvestigate the effect of cell permeable SOCS3, a cancer cell line whereendogenous SOCS3 expression is suppressed should be selected or appliedto a model, and therefore, the present experiments were performed.

Genomic DNA was extracted from cancer cell line using an Exgene™ TissueSV mini kit (Geneall®, Korea). DNA was quantified, and experiments wereperformed using 500 ng of gDNA and an EZ DNA Methylation-Gold™ kit (ZYMOResearch, Orange, Calif., USA) according to the manufacturer'sinstructions. DNA was used to perform PCR, and methylation andunmethylation of endogenous SOCS3 were qualitatively analyzed byelectrophoresis. In this regard, the primers used are as follows.

Unmethyl-F was 5′-tag tgt gta agt tgt agg aga gtg g-3′(SEQ ID NO: 816),Unmethyl-R was 5′-cta aac ata aaa aaa taa cac taa tcc aaa-3′ (SEQ ID NO:817), Methyl-F was 5′-gta gtg cgt aag ttg tag gag agc-3′ (SEQ ID NO:818), Methyl-R was 5′-gta aaa aaa taa cgc taa tcc gaa-3′ (SEQ ID NO:819), PCR was performed for 30 cycles consisting of pre-denaturation at95° C. for 5 minutes, denaturation at 95° C. for 30 seconds, annealingat 60° C. for 45 seconds, extension at 72° C. for 1 minute, and finalextension at 72° C. for 8 minutes.

As shown in FIG. 96, unmethylation of SOCS3 was observed in HEK293 andHaCaT cells which are normal cells, whereas hypermethylation of thepromoter region of SOCS3 gene was observed in HCT116, SW480, RKO, HT29which are colorectal cell lines (U: unmethylated SOCS3, M: methylatedSOCS3). These results indicate that SOCS3 is silenced byhypermethylation in colorectal cancer cell lines.

Example 17-2-2. Analysis of Expression Level of Endogenous SOCS3 mRNA inColorectal Cancer Cell Line

SOCS3 mRNA expression levels in cancer cells were analyzed by RT-PCR.mRNAs were isolated from normal cell lines and cancer cell linesaccording to a method provided in a manufacturer's sheet of Hybrid-R(Geneall, Korea), and PCR was performed using SOCS3 primer F 5′-cct actgaa ccc tcc tcc ga-3′ (SEQ ID NO: 820) and SOCS3 primer R 5′-gca get gggtga ctt tct ca-3′ (SEQ ID NO: 821) for 30 cycles consisting ofdenaturing (95° C.), annealing (60° C.), and extending (72° C.) for 45seconds each.

The results of electrophoresis showed that high expression levels ofSOCS3 were observed in the normal HEK293 cells whereas low expressionlevels of SOCS3 gene were observed in the colorectal cancer cell lines,SW480 and HT29 (FIG. 61).

Example 17-2-3. Analysis of Expression of Endogenous SOCS3 and JAK/STATSignaling Activation Status in Colorectal Cancer Cell Line

JAK/STAT3 activation in cancer cells was analyzed by Western blotanalysis. The normal HEK293 cells, and colorectal cancer cell lines,HCT116, SW480, RKO, and HT29 were washed with PBS, and then the cellswere lysed in RIPA lysis buffer (Biosesang, Seongnam, Korea) containingproteinase inhibitor cocktail (Roche, Indianapolis, Ind., USA),incubated for 15 min at 4° C., and centrifuged at 13,000 rpm for 10 minat 4° C. to isolate proteins, followed by western blotting in the samemanner as in Example 8-1.

As shown in FIG. 62, high expression level of SOCS3 and lowphosphorylation levels of p-JAK1, p-JAK2, p-STAT1, and p-STAT3 wereobserved in normal HEK293 cells. Low expression level of SOCS3 gene wasobserved in the colorectal cancer cell lines, compared to the normalcell line, and similar levels of p-JAK1, p-JAK2, and p-STAT3 wereobserved in HCT116 and HT29. These results suggest a possibility ofdeveloping a mechanism-specific anticancer agent, becauseSOCS3-deficient cancer cells can be replenished with cell permeableproteins, and activated JAK/STAT-signaling can be negatively regulated.

Example 17-3. Investigation of Anticancer Efficacy of iCP-SOCS3Recombinant Protein (Glioblastoma Cells)

To develop the iCP-SOCS3 recombinant protein as a mechanism-specifictherapeutic agent for solid tumors, SOCS3 levels endogenously expressedand activation of JAK/STAT-signaling pathway were investigated indifferent glioblastoma cells, normal cells, and normal hepatic cells.

Example 17-3-1. Analysis of Hypermethylation Level in Glioblastoma CellLine

SOCS3 expression is suppressed due to methylation in cancer cells, andtherefore, inflammation or cancer development is increased. Further, toinvestigate the effect of cell permeable SOCS3, a cancer cell line whereendogenous SOCS3 expression is suppressed should be selected or appliedto a model, and therefore, the present experiments were performed.

Genomic DNA was extracted from cancer cell line using an Exgene™ TissueSV mini kit (Geneall®, Korea). DNA was quantified, and experiments wereperformed using 500 ng of gDNA and an EZ DNA Methylation-Gold™ kit (ZYMOResearch, Orange, Calif., USA) according to the manufacturer'sinstructions. DNA was used to perform PCR, and methylation andunmethylation of endogenous SOCS3 were qualitatively analyzed byelectrophoresis. In this regard, the primers used are as follows.

Unmethyl-F was 5′-tag tgt gta agt tgt agg aga gtg g-3′(SEQ ID NO: 816),Unmethyl-R was 5′-cta aac ata aaa aaa taa cac taa tcc aaa-3′ (SEQ ID NO:817), Methyl-F was 5′-gta gtg cgt aag ttg tag gag agc-3′ (SEQ ID NO:818), and Methyl-R was 5′-gta aaa aaa taa cgc taa tcc gaa-3′ (SEQ ID NO:819). PCR was performed for 30 cycles consisting of pre-denaturation at95° C. for 5 minutes, denaturation at 95° C. for 30 seconds, annealingat 60° C. for 45 seconds, and extension at 72° C. for 1 minute, and thenfinal extension at 72° C. for 8 minutes.

As shown in FIG. 63, unmethylation of SOCS3 was observed in HEK293 andHaCaT cells which are normal cells, whereas hypermethylation of thepromoter region of SOCS3 gene was observed in U-87 MG, U-118 MG, T98G,LN229 which are glioblastoma cell lines (U: unmethylated SOCS3, M:methylated SOCS3). These results indicate that SOCS3 is silenced byhypermethylation in glioblastoma cell lines.

Example 17-3-2. Analysis of Expression of Endogenous SOCS3 and JAK/STATSignaling Activation Status in Glioblastoma Cells

JAK/STAT3 activation in cancer cells was analyzed by Western blotanalysis. The normal HaCaT and HEK293 cells, and glioblastoma cells,U-87 MG, U-118 MG, T98G, and LN229 were washed with PBS, and then thecells were lysed in RIM lysis buffer (Biosesang, Seongnam, Korea)containing protease inhibitor cocktail (Roche, Indianapolis, Ind., USA),incubated for 15 min at 4° C., and centrifuged at 13,000 rpm for 10 minat 4° C. to isolate proteins, followed by western blotting in the samemanner as in Example 8-1.

As shown in FIG. 64, high expression levels of SOCS3 and lowphosphorylation levels of p-STAT1, p-STAT3, and p-p65 were observed innormal HaCaT and HEK293 cells. Low expression level of SOCS3 gene wasobserved in the glioblastoma cell lines, compared to the normal cellline, and high levels of p-STAT1 and p-STAT3 were observed in LN229 andT98G. High levels of P-p65 were observed in all 4 types of glioblastomacell lines. These results suggest a possibility of developing amechanism-specific anticancer agent, because SOCS3-deficient cancercells can be replenished with cell permeable proteins, and activatedJAK/STAT-signaling can be negatively regulated.

Example 17-4. Investigation of Anticancer Efficacy of iCP-SOCS3Recombinant Protein

To develop the iCP-SOCS3 recombinant protein as a mechanism-specifictherapeutic agent for solid tumors, SOCS3 levels endogenously expressedand activation of JAK/STAT-signaling pathway were investigated indifferent breast cancer cell lines, normal cells, and normal hepaticcells.

Example 17-4-1. Analysis of Expression Level of Endogenous SOCS3 mRNA inBreast Cancer Cell Line

SOCS3 mRNA expression levels in cancer cells were analyzed by RT-PCR.mRNAs were isolated from normal cell lines and cancer cell linesaccording to a method provided in a manufacturer's sheet of Hybrid-R(Geneall, Korea), and PCR was performed using SOCS3 primer F 5′-cct actgaa ccc tcc tcc ga-3′ (SEQ ID NO: 820) and SOCS3 primer R 5′-gca get gggtga ctt tct ca-3′ (SEQ ID NO: 821) for 30 cycles consisting ofdenaturing (95° C.), annealing (60° C.), and extending (72° C.) for 45seconds each.

The results of electrophoresis showed that high expression levels ofSOCS3 were observed in the normal HEK293 cells whereas low expressionlevels of SOCS3 were observed in the breast cancer cell lines, exceptMDA-MB-231 (FIG. 65).

Example 17-4-2. Analysis of Hypermethylation Level in Breast Cancer CellLine

SOCS3 expression is suppressed due to methylation in cancer cells, andtherefore, inflammation or cancer development is increased. Further, toinvestigate the effect of cell permeable SOCS3, a cancer cell line whereendogenous SOCS3 expression is suppressed should be selected or appliedto a model, and therefore, the present experiments were performed.

Genomic DNA was extracted from cancer cell line using an Exgene™ TissueSV mini kit (Geneall®, Korea). DNA was quantified, and experiments wereperformed using 500 ng of gDNA and an EZ DNA Methylation-Gold™ kit (ZYMOResearch, Orange, Calif., USA) according to the manufacturer'sinstructions. DNA was used to perform PCR, and methylation andunmethylation of endogenous SOCS3 were qualitatively analyzed byelectrophoresis. In this regard, the primers used are as follows.

Unmethyl-F was 5′-tag tgt gta agt tgt agg aga gtg g-3′(SEQ ID NO: 816),Unmethyl-R was 5′-cta aac ata aaa aaa taa cac taa tcc aaa-3′ (SEQ ID NO:817), Methyl-F was 5′-gta gtg cgt aag ttg tag gag agc-3′ (SEQ ID NO:818), and Methyl-R was 5′-gta aaa aaa taa cgc taa tcc gaa-3′ (SEQ ID NO:819). PCR was performed for 30 cycles consisting of pre-denaturation at95° C. for 5 minutes, denaturation at 95° C. for 30 seconds, annealingat 60° C. for 45 seconds, and extension at 72° C. for 1 minute, and thenfinal extension at 72° C. for 8 minutes.

As shown in FIG. 66, unmethylation of SOCS3 was observed in HEK293 cellswhich are normal cells, whereas hypermethylation of the promoter regionof SOCS3 gene was observed in breast cancer cell lines, MDA-MB-231,MCF7, SK-BR3, and T47D (U: unmethylated SOCS3, M: methylated SOCS3).These results indicate that SOCS3 is silenced by hypermethylation inbreast cancer cell lines.

Example 17-4-3. Analysis of Expression of Endogenous SOCS3 and JAK/STATSignaling Activation Status in Breast Cancer Cell Line

JAK/STAT3 activation in cancer cells was analyzed by Western blotanalysis. The normal HEK293 cells, and breast cancer cell lines,MDA-MB-231, MCF7, SK-BR3, and T47D were washed with PBS, and then thecells were lysed in RIPA lysis buffer (Biosesang, Seongnam, Korea)containing protease inhibitor cocktail (Roche, Indianapolis, Ind., USA),incubated for 15 min at 4° C., and centrifuged at 13,000 rpm for 10 minat 4° C. to isolate proteins, followed by western blotting in the samemanner as in Example 8-1.

As shown in FIG. 67, high expression levels of SOCS3 and lowphosphorylation levels of p-STAT1 and p-STAT3 were observed in normalHEK293 cells. Low expression level of SOCS3 gene was observed in thebreast cancer cell lines, compared to the normal cell line. Further,high levels of p-STAT1 were observed in SK-BR3 and T47D, and high levelsof p-STAT3 were observed in MDA-MB-231 and T47D. These results suggest apossibility of developing a mechanism-specific anticancer agent, becauseSOCS3-deficient cancer cells can be replenished with cell permeableproteins, and activated JAK/STAT-signaling can be negatively regulated.

Example 18. Investigation of Anti-Cancer Efficacy of iCP-SOCS3 Example18-1. Investigation of Anti-Cancer Efficacy (Anti-ProliferativeActivity) of iCP-SOCS3

In order to develop the iCP-SOCS3 recombinant protein as a therapeuticagent for solid tumors, efficacy of iCP-SOCS3 on proliferation of solidtumors (gastric cancer, colorectal cancer, breast cancer, andglioblastoma cell lines) was investigated.

Cells originated from human gastric cancer cells (AGS, MKN45, MKN75,NCI-N87), colorectal cancer cells (HCT116), glioblastoma (U-87 MG),breast cancer cells (MDA-MB-231), and mouse fibroblast (NIH3T3) werepurchased from ATCC (Manassas, Va., USA) and KCLB (Seoul, Korea). Allthe cells were maintained as recommended by the supplier. These cells(3×10³/well) were seeded in 96 well plates. The next day, cells weretreated with DMEM (vehicle) or 10 μM recombinant SOCS3 proteins for 96hrs in the presence of serum (2%). Proteins were replaced daily. Cellgrowth and survival were evaluated with the CellTiter-Glo Cell ViabilityAssay (Promega, Madison, Wis.). Measurements using a Luminometer (TurnerDesigns, Sunnyvale, Calif.) were conducted following the manufacturer'sprotocol.

Since the endogenous level of SOCS3 protein is reduced in solid tumorpatient, and SOCS3 negatively regulates cell growth and motility incultured solid tumor cells, we investigated whether iCP-SOCS3 inhibitscell viability through SOCS3 intracellular replacement in solid tumorcells. As shown in FIG. 68, SOCS3 recombinant proteins containingaMTD₁₆₅ significantly suppressed cancer cell proliferation. HM₁₆₅S3B(iCP-SOCS3) protein was the most cytotoxic to gastric cancer cells—over40% in 10 μM treatment (p<0.01)—especially compared to vehicle alone(i.e. exposure of cells to culture media without recombinant proteins;left).

As shown in FIG. 69, SOCS3 recombinant proteins containing aMTD₁₆₅significantly suppressed cancer cell proliferation. HM₁₆₅S3B (iCP-SOCS3)protein was the most cytotoxic to colorectal cancer cells—over 60% in 10μM treatment (p<0.01)—especially compared to vehicle alone (i.e.exposure of cells to culture media without recombinant proteins; left).

As shown in FIG. 70, SOCS3 recombinant proteins containing aMTD₁₆₅significantly suppressed cancer cell proliferation. HM₁₆₅S3B (iCP-SOCS3)protein was the most cytotoxic to glioblastoma cells—over 80% in 10 μMtreatment (p<0.01)—especially compared to vehicle alone (i.e. exposureof cells to culture media without recombinant proteins; left).

As shown in FIG. 71, SOCS3 recombinant proteins containing aMTD₁₆₅significantly suppressed cancer cell proliferation. HM₁₆₅S3B (iCP-SOCS3)protein was the most cytotoxic to breast cancer cells—over 40% in 10 μMtreatment (p<0.01)—especially compared to vehicle alone (i.e. exposureof cells to culture media without recombinant proteins; left).

However, neither cell-permeable SOCS3 protein adversely affected thecell viability of non-cancer cells (NIH3T3) even after exposing thesecells to equal concentrations (10 μM) of protein over 4 days. Theseresults suggest that the iCP-SOCS3 protein is not overly toxic to normalcells and selectively kills tumor cells, and would have a great abilityto inhibit cell survival-associated phenotypes in various cancer withoutany severe aberrant effects as a protein-based biotherapeutics.

Example 18-2. Investigation of Anti-Cancer Efficacy (Cell MigrationInhibition) of iCP-SOCS3

In order to develop the iCP-SOCS3 recombinant protein as a therapeuticagent for solid tumors, efficacy of iCP-SOCS3 on migration andmetastasis of solid tumors (gastric cancer, colorectal cancer, breastcancer, and glioblastoma cell lines) was investigated.

Cells were seeded into 12-well plates, grown to 90% confluence, andincubated with 10 μM Non-CP-SOCS3 (HS3B), iCP-SOCS3 (HM₁₆₅S3B) inserum-free medium for 2 hrs prior to changing the growth medium(recommended medium by the supplier). The cells were washed twice withPBS, and the monolayer at the center of the well was “wounded” byscraping with a pipette tip. Cells were cultured for an additional 24-48hrs and cell migration was observed by phase contrast microscopy (Nikon,ECLIPSE Ts2). The migration was quantified by counting the number ofcells that migrated from the wound edge into the clear area.

As shown in FIGS. 72-75, iCP-SOCS3 (HM₁₆₅S3B) suppressed therepopulation of wounded monolayer although SOCS3 protein lacking aMTD₁₆₅(HS3B) had no effect on the cell migration, in AGS (FIG. 72), HCT116(FIG. 73), U-87 MG (FIG. 74), and MDA-MB-231 (FIG. 75), respectly. Innormal cells (NIH3T3), iCP-SOCS3 (HM₁₆₅S3B) had no effect on the cellmigration.

Transwell Migration Assay

The lower surface of Transwell inserts (Costar) was coated with 0.1%gelatin, and the membranes were allowed to dry for 1 hr at roomtemperature. The Transwell inserts were assembled into a 24-well plate,and the lower chamber was filled with growth media containing 10% FBSand FGF2 (40 ng/ml). Cells were added to each upper chamber at a densityof 5×10⁵, and the plate was incubated at 37° C. in a 5% CO₂ incubatorfor 24 hrs. Migrated cells were stained with 0.6% hematoxylin and 0.5%eosin and counted.

As shown in FIGS. 76 (left) and 77, AGS (FIGS. 76) and U-87 MG (FIG. 77)cells treated with iCP-SOCS3 (HM₁₆₅S3B) protein also showed significantinhibitory effect on their Transwell migration compared with untreatedcells (Vehicle) and non-permeable SOCS3 protein-treated cells.

Invasion Assay

The lower surface of Transwell inserts (Costar) was coated with 0.1%gelatin, the upper surface of Transwell inserts was coated with matrigel(40 μg per well; BD Pharmingen, San Diego, Calif., USA), and themembranes were allowed to dry for 1 hr at room temperature. TheTranswell inserts were assembled into a 24-well plate, and the lowerchamber was filled with growth media containing 10% FBS and FGF2 (40ng/ml). Cells (5×10⁵) were added to each upper chamber, and the platewas incubated at 37° C. in a 5% CO₂ incubator for 24 hrs. Migrated cellswere stained with 0.6% hematoxylin and 0.5% eosin and counted.

As shown in FIG. 76 (right), AGS treated with iCP-SOCS3 (HM₁₆₅S3B)protein caused remarkable decrease in invasion compared with the controlproteins. Taken together, these data indicate that iCP-SOCS3 contributesto inhibit tumorigenic activities of solid tumors.

Example 18-3. Investigation of Anti-Cancer Efficacy (Induction ofApoptosis) of iCP-SOCS3

To further determine the effect of iCP-SOCS3 on the tumorigenicity ofsolid tumors, we subsequently investigated whether iCP-SOCS3 regulatesapoptosis in various cancer cells, such as gastric cancer cells (AGS andMKN45), colorectal cancer cells (HCT116), glioblastoma cells (U-87 MG),and breast cancer cells (MDA-MB-231).

Annexin V/7-Aminoactinomycin D (7-AAD) staining was performed using flowcytometry according to the manufacturer's guidelines (BD Pharmingen, SanDiego, Calif., USA). Briefly, 1×10⁶ cells were washed three times withice-cold PBS. The cells were then resuspended in 100 μl of bindingbuffer and incubated with 1 μl of 7-AAD and 1 μl of Annexin V-7-AAD for30 min in the dark at 4° C. Flow cytometric analysis was immediatelyperformed using a guava easyCyte™ 8 Instrument (Merck Millipore,Darmstadt, Germany). In this regard, gemcitabine (0.1˜10 μM) was used asa reference drug.

As shown in FIG. 78, 10 uM of iCP-SOCS3 (HM₁₆₅S3B) protein did notinduce apoptosis in normal cell lines (NIH3T3 and HEK293). Whereas, asshown in FIGS. 79-82, 10 uM iCP-SOCS3 (HM₁₆₅S3B) was efficient inducerof apoptosis in cancer cells, as assessed by Annexin V staining.Consistently, no changes in Annexin V staining were observed in cancercells treated with HS3B compared to untreated cell (Vehicle).

Example 18-4. Investigation of Anti-Cancer Efficacy (Induction ofApoptosis) of iCP-SOCS3

To further determine the effect of iCP-SOCS3 on the tumorigenicity ofsolid tumors, we subsequently investigated whether iCP-SOCS3 regulatesapoptosis in colorectal cancer cells.

Apoptotic cells were analyzed using terminal dUTP nick-end labeling(TUNEL) assay with In Situ Cell Death Detection kit TMR red (Roche, 4056Basel, Switzerland). Cells were treated for 24 hr with 10 μM HS3B orHM₁₆₅S3B proteins with 2% fetal bovine serum and apoptotic cells werevisualized by TUNEL staining. Treated cells were washed with cold PBStwo times, fixed in 4% paraformaldehyde (PFA, Junsei, Tokyo, Japan) for1 hr at room temperature, and incubated in 0.1% Triton X-100 for 2 minon the ice. Cells were washed with cold PBS twice, and treated TUNELreaction mixture for 1 hr at 37° C. in dark, washed cold PBS three timesand observed by fluorescence microscopy (Nikon, Tokyo, Japan).

iCP-SOCS3 (HM₁₆₅S3B) protein was considerably efficient inducer ofapoptosis in HCT116 cells (FIG. 83), as assessed by a fluorescentterminal dUTP nick-end labeling (TUNEL) assay. Consistently, no changesin TUNEL was observed in HCT116 cells treated with HS3B compared tountreated cell (Vehicle).

Example 18-5. Investigation of Anti-Cancer Efficacy (Inhibition of CellCycle Progression) of iCP-SOCS3

To further determine the effect of iCP-SOCS3 on the tumorigenicity ofsolid tumors, we subsequently investigated whether iCP-SOCS3 regulatescell cycle progression in various cancer cells, such as gastric cancercells (AGS), colorectal cancer cells (HCT116), glioblastoma cells (U-87MG, and breast cancer cells (MDA-MB-231).

Cancer cells and normal cells (NIH3T3, HaCaT, and HEK293) were treatedwith 10 uM protein (Non-CP-SOCS3 (HS3B) and iCP-SOSC3 (HM₁₆₅S3B)) for 8hrs. After the treatment, cells were washed twice with cold PBS andre-suspended in 1 ml cold PBS, fixed in cold 70% ethanol, washed withcold PBS twice and re-suspended in PI master mix (PI 10 ug/ml (sigma),50 ug/ml RNase A (invitrogen) in staining buffer) at a final celldensity of 2×10⁵ cell/ml. The cell mixtures were incubated 30 min in thedark at 4° C. Flow cytometric analysis was immediately performed using aguava easyCyte™ 8 Instrument (Merck Millipore, Darmstadt, Germany).

iCP-SOCS3 (HM₁₆₅S3B) protein efficiently inhibited cell cycleprogression in various cancer cells, such as AGS, HCT116, U-87 MG, andMDA-MB-231 (FIGS. 85 to 88), while iCP-SOCS3 (HM₁₆₅S3B) protein did notinhibit cell cycle progression in normal cells (FIG. 84).

Example 18-6. Investigation of Anti-Cancer Efficacy (Effect on MolecularMechanism-Mode of Action) of iCP-SOCS3

To further determine the effect of iCP-SOCS3 on the tumorigenicity ofsolid tumors, we subsequently investigated whether iCP-SOCS3 regulatesapoptosis in breast cancer cells (MDA-MB-231).

Cells were treated for 24 hr with 10 μM HS3B or HM₁₆₅S3B proteins, lysedin RIPA lysis buffer containing proteinase inhibitor cocktail, incubatedfor 15 min at 4° C., and centrifuged at 13,000 rpm for 10 min at 4° C.Equal amounts of lysates were separated on 15% SDS-PAGE gels andtransferred to a nitrocellulose membrane. The membranes were blockedusing 5% skim milk or 5% Albumin in TBST and incubated with thefollowing antibodies: anti-Bc1-2 (Santa Cruz biotechnology),anti-Cleaved Caspase 3 (Cell Signaling Technology), then HRP conjugatedanti-mouse or anti-rabbit secondary antibody. The expression of eachprotein was determined by immunoblotting with indicated antibodies. Anantibody against β-actin was used as a loading control.

MDA-MB-231 cells treated with iCP-SOCS3 (HM₁₆₅S3B) protein dramaticallyreduced the expression of anti-apoptotic protein such as B-cell lymphoma2 (Bc1-2) and increased the level of cleaved cysteine-aspartic acidprotease (caspase-3) at 24 hr (FIG. 89). These results indicate thatiCP-SOCS3 induces apoptosis of breast cancer cells and may suppress thecancer progression by this pathway.

Example 19. Investigation of Anti-Cancer Efficacy of iCP-SOCS3 inXenograft Model (Cell-Derived Xenograft (CDX)-Model) Example 19-1.Investigation of Anti-Cancer Efficacy of iCP-SOCS3 in Gastric CancerCell (NCI-N87)-Derived Xenograft (CDX) Model

In the following experiments, Western blotting was performed in the samemanner as in Example 8-1, and RT-PCR and MC were performed by thefollowing method.

RT-PCR

Tumor tissues were finely minced using a homogenizer according to themanufacturer's protocol of Hybrid-R (geneall, Korea), and then mRNA wasisolated therefrom. 1 ug of mRNA thus separated was used to synthesizecDNA using an Accupower RT Premix (Bioneer, Korea). PCR was performedusing 2 ul of cDNA and the primers of Table 45. PCR was performed usingan Accupower PCR Premix (Bioneer, Korea) for 30 cycles consisting ofdenaturing (95° C.), annealing (60° C.), and extending (72° C.) for 45seconds each.

TABLE 45 Genes Forward Sequence Reverse Sequence CyclinCCGTTTACAAGCTAAGCAGC GTGGTTCCAAGTCAGAATGC E (SEQ ID NO: 839)(SEQ ID NO: 840) Cyclin TCAGTACTTGAGGCGACAAGG CTCCCTAATTGCTTGCTGAGG A1(SEQ ID NO: 841) (SEQ ID NO: 842) Bax CCCTTTTGCTTCAGGGTTTCGCCACTCGGAAAAAGACCTC (SEQ ID NO: 843) (SEQ ID NO: 844) FAKTGGTGAAAGCTGTCATCGAG CTGGGCCAGTTTCATCTTGT (SEQ ID NO: 845)(SEQ ID NO: 846) p21 CAGCGGAACAAGGAGTCAGA AGAAACGGGAACCAGGACAC(SEQ ID NO: 847) (SEQ ID NO: 848) p27 GATAATCCCGCTCTGAATGCGCTTCTCTTAGTGCTGTAGC (SEQ ID NO: 849) (SEQ ID NO: 850) VEGFCTTCAAGCCATCCTGTGTGC ACGCGAGTCTGTGTTTTTGC (SEQ ID NO: 851)(SEQ ID NO: 852) HIF- ATCAGACACCTAGTCCTTCCG TTGAGGACTTGCGCTTTCAGG 1α(SEQ ID NO: 853) (SEQ ID NO: 854) GAPDH AAGGGTCATCATCTCTGCCCGTGATGGCATGGACTGTGGT (SEQ ID NO: 855) (SEQ ID NO: 856)

Immunohistochemistry (IHC)

Tissue samples were fixed in 4% Paraformaldehyde (Duksan, South Korea)for 3 days, dehydrated, cleared with xylene and embedded in Paraplast.Sections (6 μm thick) of tumor were placed onto poly-L-lysine coatedslides. To block endogenous peroxidase activity, sections were incubatedfor 15 min with 3% H₂O₂ in methanol. After washing three times with PBS,slides were incubated for 30 min with blocking solution (5% fetal bovineserum in PBS). Mouse anti-Bax antibody (sc-7480, Santa CruzBiotechnology, SantaCruz, Calif., USA) and rabbit anti-VEGF (ab46154,Abcam, Cambridge, UK) were diluted 1:1000 (to protein concentration 0.1μg/ml) in blocking solution, applied to sections, and incubated at 4° C.for 24 hrs. After washing three times with PBS, sections were incubatedwith biotinylated mouse and rabbit IgG (Vector Laboratories, Burlingame,Calif., USA) at a 1:1000 dilution for 1 hr at room temperature, thenincubated with avidin-biotinylated peroxidase complex using aVectorstain ABC Kit (Vector Laboratories, Burlingame, Calif., USA) for30 min at room temperature. After the slides are reacted with oxidized3, 3-diaminobenzidine as a chromogen, they were counterstained withHarris hematoxylin (Sigma-Aldrich, USA). Permanently mounted slides wereobserved and photographed using a microscope equipped with a digitalimaging system (ECLIPSE Ti, Nikon, Japan).

Anti-tumor activity of iCP-SOCS3 against human cancer xenografts wasassessed. Female Balb/c^(mu/mu) mice (5-week-old, Doo-Yeol Biotech Co.Ltd. Korea) were subcutaneously implanted with Hep3B2.1-7 tumor block (1mm³) into the left back side of the mouse. Tumor-bearing mice wereintravenously administered with 600 μg/head of iCP-SOCS3 (HM₁₆₅S3B) orthe control proteins (HS3B) for 21 days and observed for 2 weeksfollowing the termination of the treatment. After protein treatment,mice were killed, and six organs (brain, heart, lung, liver, kidney, andspleen) from each were collected and kept in a suitable fixationsolution until the next step. Tumor size was monitored by measuring thelongest (length) and shortest dimensions (width) once a day with a dialcaliper, and tumor volume was calculated as width2×length×0.5.

As shown in FIG. 90, HM₁₆₅S3B protein significantly suppressed the tumorgrowth (p<0.05) during the treatment and the effect persisted for atleast 2 weeks after the treatment was terminated (65% inhibition at day35, respectively). Whereas, the growth of HS3B-treated tumors increased,matching the rates observed in control mice (Vehicle). These resultssuggest that iCP-SOCS3 inhibits the growth of established tumors as wellas the tumor growth of gastric cancer cells.

mRNA levels were analyzed using the tumor tissues removed at 2 weeks(Day 35) after termination of drug administration to analyze changes inexpressions of major biomarker genes by iCP-SOCS3.

Expressions of cell cycle (cyclinAl) and angiogenesis (VEGF)-relatedgenes were significantly regulated by treatment of iCP-SOCS3.

Accordingly, it was confirmed that iCP-SOCS3 is able to inhibitdevelopment of gastric cancer through expressions of various factorsregulating cancer activity such as cell cycle, angiogenesis, etc.

Further, tumor tissues removed at 2 weeks (Day 35) after termination ofdrug administration were used to perform Western blotting and IHC, andincrease or decrease in the expressions of cancer activity regulatingfactors by iCP-SOCS3 was observed.

In iCP-SOCS3-treated tumor tissues, expressions of a cell cycleregulator, p21 and apoptosis inducers, Bax and cleaved caspase-3 wereincreased. Further, expression of an angiogenesis inducer VEGF was alsoremarkably decreased (FIG. 90). Accordingly, it was demonstrated thatcell cycle, apoptosis, and angiogenesis are also regulated by iCP-SOCS3in-vivo.

Example 19-2. Investigation of Anti-Cancer Efficacy of iCP-SOCS3 inColorectal Cancer Cell (HCT116)-Derived Xenograft (CDX) Model

The following experiments were performed in the same manner as inExample 19-1.

As shown in FIG. 91, HM₁₆₅S3B protein significantly suppressed the tumorgrowth (p<0.05) during the treatment and the effect persisted for atleast 2 weeks after the treatment was terminated (79% at day 35,respectively). Whereas, the growth of HS3B-treated tumors increased,matching the rates observed in control mice (Vehicle). These resultssuggest that iCP-SOCS3 inhibits the growth of established tumors as wellas the tumor growth of colorectal cancer cells.

mRNA levels were analyzed using the tumor tissues removed at 2 weeks(Day 35) after termination of drug administration to analyze changes inexpressions of major biomarker genes by iCP-SOCS3.

Expressions of apoptosis (Bax) and angiogenesis (VEGF)-related geneswere significantly regulated by treatment of iCP-SOCS3.

Accordingly, it was confirmed that iCP-SOCS3 is able to inhibitdevelopment of colorectal cancer through expressions of various factorsregulating cancer activity such as apoptosis, angiogenesis, etc.

Further, tumor tissues removed at 2 weeks (Day 35) after termination ofdrug administration were used to perform Western blotting and IHC, andincrease or decrease in the expressions of cancer activity regulatingfactors by iCP-SOCS3 was observed.

In iCP-SOCS3-treated tumor tissues, expressions of a cell cycleregulator, p21 and an apoptosis inducer, Bax were increased. Further,expression of an angiogenesis inducer VEGF was also remarkably decreased(FIG. 91). Accordingly, it was demonstrated that cell cycle, apoptosis,and angiogenesis are also regulated by iCP-SOCS3 in-vivo.

Example 19-3. Investigation of Anti-Cancer Efficacy of iCP-SOCS3 inGlioblastoma Cell (U-87 MG)-Derived Xenograft (CDX) Model

The following experiments were performed in the same manner as inExample 19-1.

As shown in FIG. 92, HM₁₆₅S3B protein significantly suppressed the tumorgrowth (p<0.05) during the treatment and the effect persisted for atleast 2 weeks after the treatment was terminated (76% at day 42,respectively). Whereas, the growth of HS3B-treated tumors increased,matching the rates observed in control mice (Vehicle). These resultssuggest that iCP-SOCS3 inhibits the growth of established tumors as wellas the tumor growth of glioblastoma cells.

mRNA levels were analyzed using the tumor tissues removed at 3 weeks(Day 42) after termination of drug administration to analyze changes inexpressions of major biomarker genes by iCP-SOCS3.

Expressions of apoptosis (Bax), cell cycle (CDK4, CDK2, cyclinE,cyclinAl), angiogenesis (HIF1a, VEGF), and migration (FAK)-related geneswere significantly regulated by treatment of iCP-SOCS3.

Expressions of NSE, FSTL-1, and GFAT which are biomarker genes ofglioblastoma were remarkably decreased by iCP-SOCS3.

Expressions of VEGF and angiopoietin I which are biomarker genes ofangiogenesis were significantly decreased by treatment of iCP-SOCS3.

Accordingly, it was confirmed that iCP-SOCS3 is able to inhibitdevelopment of glioblastoma through expressions of various factorsregulating cancer activity such as apoptosis, cell cycle, migration,angiogenesis, etc.

Example 19-4. Investigation of Anti-Cancer Efficacy of iCP-SOCS3 inBreast Cancer Cell (MDA-MB-231)-Derived Xenograft (CDX) Model

The following experiments were performed in the same manner as inExample 19-1.

As shown in FIG. 93, HM₁₆₅S3B protein significantly suppressed the tumorgrowth (p<0.05) during the treatment and the effect persisted for atleast 2 weeks after the treatment was terminated (63% at day 35,respectively). Whereas, the growth of HS3B-treated tumors increased,matching the rates observed in control mice (Vehicle). These resultssuggest that iCP-SOCS3 inhibits the growth of established tumors as wellas the tumor growth of breast cancer cells.

mRNA levels were analyzed using the tumor tissues removed at 2 weeks(Day 35) after termination of drug administration to analyze changes inexpressions of major biomarker genes by iCP-SOCS3.

Expressions of cell cycle (p21, p2′7, cyclinE), proliferation (PCNA),and angiogenesis (HIF1a, VEGF)-related genes were significantlyregulated by treatment of iCP-SOCS3.

Accordingly, it was confirmed that iCP-SOCS3 is able to inhibitdevelopment of breast cancer through expressions of various factorsregulating cancer activity such as cell cycle, angiogenesis, etc.,indicating characteristics of iCP-SOCS3 as a mechanism-specifictherapeutic agent targeting solid tumors.

Further, tumor tissues removed at 2 weeks (Day 35) after termination ofdrug administration were used to perform Western blotting and IHC, andincrease or decrease in the expressions of cancer activity regulatingfactors by iCP-SOCS3 was observed.

In iCP-SOCS3-treated tumor tissues, expression of a cell cycleregulator, p21 was increased and expressions of angiogenesis inducers,VEGF and CD31 were remarkably decreased (FIG. 93). Accordingly, it wasdemonstrated that cell cycle and angiogenesis are also regulated byiCP-SOCS3 in-vivo.

Statistical Analysis

Statistical analysis and graphic presentation have been performed usingGraphPad Prism 5.01 software (GraphPad, La Jolla, Calif., USA). Allexperimental data are presented as means±SEM. Statistical significancewas analyzed by the Student's t-test or ANOVA method. Experimentaldifferences between groups were assessed using paired Student's t-tests.For animal experiments, ANOVA was used for comparing between and withingroups to determine the significance. Differences with p<0.05 areconsidered to be statistically significant.

Those skilled in the art to which the present invention pertains willappreciate that the present invention may be implemented in differentforms without departing from the essential characteristics thereof.Therefore, it should be understood that the disclosed embodiments arenot limitative, but illustrative in all aspects. The scope of thepresent invention is made to the appended claims rather than to theforegoing description, and all variations which come within the range ofequivalency of the claims are therefore intended to be embraced therein.

1. A method of treating solid tumor in a subject in need thereofcomprising: administering to the subject a therapeutically effectiveamount of an iCP-SOCS3 recombinant protein, wherein the iCP-SOCS3recombinant protein comprises a SOCS3 protein, an advanced macromoleculetransduction domain (aMTD) and a solubilization domain (SD), and theiCP-SOCS3 recombinant protein is represented by following structuralformula:A-B-C, wherein A is the aMTD, B is the SOCS3 protein, and C is the SD,wherein the aMTD has an amino acid sequence of SEQ ID NO: 122, and theSD has an amino acid sequence independently selected from the groupconsisting of SEQ ID NOs: 798, 799, 800, 801, 802, 803, and
 804. 2. Themethod of claim 1, wherein the SOCS3 protein has an amino acid sequenceof SEQ ID NO:
 814. 3. The method of claim 2, wherein the SOCS3 proteinis encoded by a polynucleotide sequence of SEQ ID NO:
 815. 4. The methodclaim 1, wherein the aMTD is encoded by a polynucleotide sequence of SEQID NO:
 362. 5. The method of claim 1, wherein the SD is encoded by apolynucleotide sequence independently selected from the group consistingof SEQ ID NOs: 805, 806, 807, 808, 809, 810, and
 811. 6. The method ofclaim 1, wherein the iCP-SOCS3 recombinant protein has a histidine-tagaffinity domain additionally fused to one end thereof.
 7. The method ofclaim 6, wherein the histidine-tag affinity domain has an amino acidsequence of SEQ ID NO:
 812. 8. The method of claim 7, wherein thehistidine-tag affinity domain is encoded by a polynucleotide sequence ofSEQ ID NO: 813.